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      1. M2-型丙酮酸激酶(PKM2)多克隆抗體

        Polyclonal Antibody to Pyruvate kinase isozymes M2 (PKM2)

        M2PK; PKM; M2-PK; PKM1; CTHBP; OIP3; PK3; PKM; TCB; THBP1; Pyruvate kinase muscle isozyme; Thyroid hormone-binding protein 1; Cytosolic thyroid hormone-binding protein; Pyruvate Kinase, Muscle

        • M2-型丙酮酸激酶(PKM2)多克隆抗體 產品包裝(模擬)
        • M2-型丙酮酸激酶(PKM2)多克隆抗體 產品包裝(模擬)
        • M2-型丙酮酸激酶(PKM2)多克隆抗體 DAB staining on IHC-P; Samples: Human Breast cancer Tissue; Primary Ab: 10μg/ml Rabbit Anti-Human PKM2 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
        • M2-型丙酮酸激酶(PKM2)多克隆抗體 DAB staining on IHC-P;
          Samples: Human Skin cancer Tissue;
          Primary Ab: 10μg/ml Rabbit Anti-Human PKM2 Antibody
          Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
          (Catalog: SAA544Rb19)
        • M2-型丙酮酸激酶(PKM2)多克隆抗體 DAB staining on IHC-P;
          Samples: Human Prostate Tissue;
          Primary Ab: 10μg/ml Rabbit Anti-Human PKM2 Antibody
          Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
          (Catalog: SAA544Rb19)
        • M2-型丙酮酸激酶(PKM2)多克隆抗體 DAB staining on IHC-P;
          Samples: Human Prostate cancer Tissue;
          Primary Ab: 10μg/ml Rabbit Anti-Human PKM2 Antibody
          Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
          (Catalog: SAA544Rb19)
        • M2-型丙酮酸激酶(PKM2)多克隆抗體 DAB staining on IHC-P;
          Samples: Human Colorectal cancer Tissue;
          Primary Ab: 10μg/ml Rabbit Anti-Human PKM2 Antibody
          Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
          (Catalog: SAA544Rb19)
        • M2-型丙酮酸激酶(PKM2)多克隆抗體 DAB staining on IHC-P;
          Samples: Human Liver cancer Tissue;
          Primary Ab: 10μg/ml Rabbit Anti-Human PKM2 Antibody
          Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
          (Catalog: SAA544Rb19)
        • M2-型丙酮酸激酶(PKM2)多克隆抗體 FITC staining on IF;
          Samples: Human MCF7 cell;
          Primary Ab: 20ug/ml Rabbit Anti-Human PKM2 Antibody
          Second Ab: 1μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
          (Catalog: SAA544Rb18)
        • PAA588Hu01.jpg Figure. Western Blot; Sample: Recombinant PKM2, Human.
        • M2-型丙酮酸激酶(PKM2)多克隆抗體 Western Blot; Sample: Human K562 cell lysate;
          Primary Ab: 2μg/ml Rabbit Anti-Human PKM2 Antibody
          Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
          (Catalog: SAA544Rb19)
        • M2-型丙酮酸激酶(PKM2)多克隆抗體 Knockout Varification:
          Lane 1: Wild-type MCF7 cell lysate;
          Lane 2: PKM2 knockout MCF7 cell lysate;
          Predicted MW: 56,58kd
          Observed MW: 60kd
          Primary Ab: 2μg/ml Rabbit Anti-Human PKM2 Antibody
          Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
          (Catalog: SAA544Rb19)
        • M2-型丙酮酸激酶(PKM2)多克隆抗體 Western Blot; Sample: Human MCF7 cell lysate;
          Primary Ab: 2μg/ml Rabbit Anti-Human PKM2 Antibody
          Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
          (Catalog: SAA544Rb19)
        • M2-型丙酮酸激酶(PKM2)多克隆抗體 Western Blot; Sample: Human Hela cell lysate;
          Primary Ab: 2μg/ml Rabbit Anti-Human PKM2 Antibody
          Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
          (Catalog: SAA544Rb19)
        • M2-型丙酮酸激酶(PKM2)多克隆抗體 Western Blot; Sample: Human HepG2 cell lysate;
          Primary Ab: 2μg/ml Rabbit Anti-Human PKM2 Antibody
          Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
          (Catalog: SAA544Rb19)
        • M2-型丙酮酸激酶(PKM2)多克隆抗體 K562 human chronic myelogenous leukemia cell line was stained with rabbit Anti-human PKM2 Polyclonal Antibody (Catalog # PAA588Hu01, filled red histogram) or Isotype control antibody (Catalog # IS067-Rb01, filled green histogram), followed by APC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog # SAA544Rb15). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.
        • Certificate 通過ISO 9001、ISO 13485質量體系認證

        特異性

        該抗體是針對PKM2的兔多克隆抗體。在免疫組織化學染色和免疫印跡實驗中能識別PKM2。

        用法

        Western blotting: 0.5-2μg/mL;
        Immunohistochemistry: 5-20μg/mL;
        Immunocytochemistry: 5-20μg/mL;
        Optimal working dilutions must be determined by end user.

        儲存

        經常使用則4°C保存。-20°C保存不超過兩年。避免反復凍融。

        穩定性

        熱穩定性以損失率顯示。損失率是由加速降解試驗決定,具體方法如下:在37°C孵育48小時,沒有顯著的降解或者沉淀產生。保質期內,在適當的條件下存儲,損失率低于5%。

        贈品

        相關產品

        編號 適用物種:Homo sapiens (Human,人) 應用(僅供研究使用,不用于臨床診斷!)
        RPA588Hu01 M2-型丙酮酸激酶(PKM2)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
        RPA588Hu02 M2-型丙酮酸激酶(PKM2)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
        RPA588Hu03 M2-型丙酮酸激酶(PKM2)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
        APA588Hu02 M2-型丙酮酸激酶(PKM2)活性蛋白 Cell?culture;?Activity?Assays.
        PAA588Hu02 M2-型丙酮酸激酶(PKM2)多克隆抗體 WB; IHC; ICC; IP.
        PAA588Hu01 M2-型丙酮酸激酶(PKM2)多克隆抗體 WB; IHC; ICC; IP.
        FAA588Hu01 抗M2-型丙酮酸激酶(PKM2)多克隆抗體 Flow cytometry.
        FAA588Hu03 抗M2-型丙酮酸激酶(PKM2)多克隆抗體 Flow cytometry.
        MAA588Hu22 M2-型丙酮酸激酶(PKM2)單克隆抗體 WB; IHC; ICC; IP.
        SEA588Hu M2-型丙酮酸激酶(PKM2)檢測試劑盒(酶聯免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
        LMA588Hu M2-型丙酮酸激酶(PKM2)單因子檢測試劑盒(流式熒光發光法) Magnetic Luminex Assay for Antigen Detection.

        參考文獻

        雜志 參考文獻
        Cancer Research Antigen Presentation by Dendritic Cells in Tumors Is Disrupted by Altered Metabolism that Involves Pyruvate Kinase M2 and Its Interaction with SOCS3 [AACR: 70189]
        Molecular BioSystems Abnormal levels of heterogeneous nuclear ribonucleoprotein A2B1 (hnRNPA2B1) in tumour tissue and blood samples from patients diagnosed with lung cancer [Pubmed:25483567]
        Tumour Biol M2 isoform of pyruvate kinase (PKM2) is upregulated in Kazakh’s ESCC and promotes proliferation and migration of ESCC cells [PubMed: 26404132]
        Oncotarget Identification of a serum biomarker panel for the differential diagnosis of cholangiocarcinoma and primary sclerosing cholangitis [Pubmed:29707118]
        Journal of?Neurotrauma Acteoside improves muscle atrophy and motor function by inducing new myokine secretion in chronic spinal cord injury [Pubmed: 30318996]
        Cell Reports Secreted Pyruvate Kinase M2 Promotes Lung Cancer Metastasis through Activating the Integrin Beta1/FAK Signaling Pathway [Pubmed: 32049010]
        Chrysin serves as a novel inhibitor of DGKα/FAK interaction to suppress the malignancy of esophageal squamous cell carcinoma (ESCC) []
        Cancer Cell International Bufalin Induced Mitochondrial Dysfunction Promotes Apoptosis of Glioma Cells by Regulating Annexin A2 and DRP1 Proteins []
        Cancer Cell Int Bufalin induces mitochondrial dysfunction and promotes apoptosis of glioma cells by regulating Annexin A2 and DRP1 protein expression [34376212]
        Life (Basel) Putative Association between Low Baseline Gene Expression in the Peripheral Blood and Clinical Remission in Rheumatoid Arthritis Patients Treated with Tofacitinib [34947916]
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