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      1. 白蛋白(ALB)檢測試劑盒(酶聯免疫吸附試驗法)

        ELISA Kit for Albumin (ALB)

        Serum albumin

        • 白蛋白(ALB)檢測試劑盒(酶聯免疫吸附試驗法) 產品包裝(模擬)
        • 白蛋白(ALB)檢測試劑盒(酶聯免疫吸附試驗法) 產品包裝(模擬)
        • 白蛋白(ALB)檢測試劑盒(酶聯免疫吸附試驗法) 實驗結果圖
        • CEB028Gu.jpg 標準曲線圖
        • Certificate 通過ISO 9001、ISO 13485質量體系認證

        特異性

        本試劑盒用于檢測白蛋白(ALB),經檢測與其它相似物質無明顯交叉反應。
        由于受到技術及樣本來源的限制,不可能完成對所有相關或相似物質交叉反應檢測,因此本試劑盒有可能與未經檢測的其它物質有交叉反應。

        回收率

        分別于定值血清及血漿樣本中加入一定量的白蛋白(ALB)(加標樣品),重復測定并計算其均值,回收率為測定值與理論值的比率。

        樣本 回收率范圍(%) 平均回收率(%)
        serum(n=5) 92-101 96
        EDTA plasma(n=5) 80-93 85
        heparin plasma(n=5) 96-103 101

        精密度

        精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
        批內差:取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續測定20 次,分別計算不同濃度樣本的平均值及SD值。
        批間差:選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定,每個樣本使用同一試劑盒重復測定8次,分別計算不同濃度樣本的平均值及SD值。
        批內差: CV<10%
        批間差: CV<12%

        線性

        在定值血清及血漿樣本內加入適量的白蛋白(ALB),并倍比稀釋成1:2,1:4,1:8,1:16的待測樣本,線性范圍即為稀釋后樣本中白蛋白(ALB)含量的測定值與理論值的比率。

        樣本 1:2 1:4 1:8 1:16
        serum(n=5) 92-101% 78-97% 80-95% 84-96%
        EDTA plasma(n=5) 96-105% 97-104% 97-104% 95-105%
        heparin plasma(n=5) 88-95% 95-102% 80-104% 94-105%

        穩定性

        經測定,試劑盒在有效期內按推薦溫度保存,其活性降低率小于5%。
        為減小外部因素對試劑盒破壞前后檢測值的影響,實驗室的環境條件需盡量保持一致,尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

        實驗流程

        1. 實驗前標準品、試劑及樣本的準備;
        2. 加樣(標準品及樣本)50µL,
            加入50µL檢測液A(臨用前配制);
            37°C溫育1小時。
        3. 洗板3次;
        4. 加檢測溶液B100µL,37°C孵育30分鐘;
        5. 洗板5次;
        6. 加TMB底物90µL,37°C孵育10-20分鐘;
        7. 加終止液50µL,立即450nm讀數。

        實驗原理

        本試劑盒應用競爭抑制酶聯免疫分析法測定標本中待測物質水平。將白蛋白(ALB)單克隆抗體包被微孔板,制成固相載 體,往包被抗體的微孔中同時加入生物素標記的抗原和待測抗原(標準品或樣本),待測抗原與生物素標記抗原對特異性抗體進行競爭結合。溫育后經洗滌去掉未結合物,然后加入HRP標記的親和素,經過溫育和徹底洗滌后加入底物TMB顯色。TMB在過氧化物酶的催化下轉化成藍色,并在酸的作用下轉化成最終的黃色。待測標本濃度越高,標記抗原和抗體的結合就越受到抑制,顯色愈淺。顯色的深淺與酶量呈正相關,而與樣品中待測物質含量呈負相關。用酶標儀在450nm波長下測定吸光度(O.D.值),計算樣品濃度。

        相關產品

        編號 適用物種:Cavia (Guinea pig ,豚鼠) 應用(僅供研究使用,不用于臨床診斷!)
        NPB028Gu01 白蛋白(ALB)天然蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
        RPB028Gu01 白蛋白(ALB)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
        PAB028Gu01 白蛋白(ALB)多克隆抗體 WB; IHC; ICC; IP.
        LAB028Gu71 白蛋白(ALB)多克隆抗體(生物素標記) WB; IHC; ICC.
        MAB028Gu21 白蛋白(ALB)單克隆抗體 WB; IHC; ICC; IP.
        CEB028Gu 白蛋白(ALB)檢測試劑盒(酶聯免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
        LMB028Gu 白蛋白(ALB)單因子檢測試劑盒(流式熒光發光法) Magnetic Luminex Assay for Antigen Detection.

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