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      1. 堿性磷酸酶(ALP)檢測試劑盒(酶聯免疫吸附試驗法)

        ELISA Kit for Alkaline Phosphatase (ALP)

        AKP; ALKP; Basic Phosphatase

        • 堿性磷酸酶(ALP)檢測試劑盒(酶聯免疫吸附試驗法) 產品包裝(模擬)
        • 堿性磷酸酶(ALP)檢測試劑盒(酶聯免疫吸附試驗法) 產品包裝(模擬)
        • 堿性磷酸酶(ALP)檢測試劑盒(酶聯免疫吸附試驗法) 實驗結果圖
        • SEB472Bo.jpg 標準曲線圖
        • Certificate 通過ISO 9001、ISO 13485質量體系認證





        樣本 回收率范圍(%) 平均回收率(%)
        serum(n=5) 96-105 101
        EDTA plasma(n=5) 87-95 91
        heparin plasma(n=5) 93-101 97


        精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
        批內差:取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續測定20 次,分別計算不同濃度樣本的平均值及SD值。
        批內差: CV<10%
        批間差: CV<12%



        樣本 1:2 1:4 1:8 1:16
        serum(n=5) 85-103% 80-101% 81-93% 83-92%
        EDTA plasma(n=5) 78-95% 89-96% 79-89% 87-101%
        heparin plasma(n=5) 91-101% 92-102% 78-97% 78-95%




        1. 實驗前標準品、試劑及樣本的準備;
        2. 加樣(標準品及樣本)100µL,37°C孵育1小時;
        3. 吸棄,加檢測溶液A100µL,37°C孵育1小時;
        4. 洗板3次;
        5. 加檢測溶液B100µL,37°C孵育30分鐘;
        6. 洗板5次;
        7. 加TMB底物90µL,37°C孵育10-20分鐘;
        8. 加終止液50µL,立即450nm讀數。




        編號 適用物種:Bos taurus; Bovine (Cattle,牛) 應用(僅供研究使用,不用于臨床診斷!)
        RPB472Bo01 堿性磷酸酶(ALP)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
        PAB472Bo01 堿性磷酸酶(ALP)多克隆抗體 WB; IHC; ICC; IP.
        LAB472Bo71 堿性磷酸酶(ALP)多克隆抗體(生物素標記) WB; IHC; ICC.
        SEB472Bo 堿性磷酸酶(ALP)檢測試劑盒(酶聯免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
        SCB472Bo 堿性磷酸酶(ALP)檢測試劑盒(化學發光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
        LMB472Bo 堿性磷酸酶(ALP)單因子檢測試劑盒(流式熒光發光法) Magnetic Luminex Assay for Antigen Detection.
        KSB472Bo01 堿性磷酸酶(ALP)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) Main materials for "Do It (ELISA Kit) Yourself".


        雜志 參考文獻
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        22 Combined effect of strontium and zoledronate on hydroxyapatite structure and bone cell responses [ScienceDirect: S0142961214003135]
        Biomed Mater.? Directing chondrogenic differentiation of mesenchymal stem cells with a solid-supported chitosan thermogel for cartilage tissue engineering [Pubmed:24770944]
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        Bone The active role of osteoporosis in the interaction between osteoblasts and bone metastases [PubMed: 26057367]
        Macromolecular Bioscience Multi‐Layered Scaffolds for Osteochondral Tissue Engineering: In Vitro Response of Co‐Cultured Human Mesenchymal Stem Cells [PubMed: 26126665]
        journal of hard Tissue Biology Biological Evaluation of a Prototype Material made of Polyglycolic Acid and Hydroxyapatite [Article: Jhtb]
        Colloids Surf B Biointerfaces Antiresorption implant coatings based on calcium alendronate and octacalcium phosphate deposited by matrix assisted pulsed laser evaporation [PubMed: 26445021]
        Gels On the Mechanism of Drug Release from Polysaccharide Hydrogels Cross-Linked with Magnetite Nanoparticles by Applying Alternating Magnetic Fields: the Case of DOXO Delivery [2310-2861: 1]
        Acta Biomater Antioxidant and bone repair properties of quercetin-functionalized hydroxyapatite: an in vitro osteoblast-osteoclast-endothelial cell co-culture study [PubMed: 26689470]
        Sci Rep A composite scaffold of MSC affinity peptide-modified demineralized bone matrix particles and chitosan hydrogel for cartilage regeneration [PubMed: 26632447]
        Acta Biomaterialia Antioxidant and bone repair properties of quercetin-functionalized hydroxyapatite: An in vitro osteoblast–osteoclast–endothelial cell co-culture study [Pubmed:26689470]
        PLoS One. Extracorporeal shock waves alone or combined with raloxifene promote bone formation and suppress resorption in ovariectomized rats. [pubmed:28158228]
        Scientific Reports Adiponectin?protects?the?rats?liver?against?chronic?intermittent?hypoxia?induced?injury?throughAMP-activated?protein?kinase?pathway. [pubmed:27678302]
        J Cell Physiol. An advanced tri-culture model to evaluate the dynamic interplay among osteoblasts, osteoclasts, and endothelial cells. [pubmed:28240358]
        Biomedicine & Pharmacotherapy Anti-osteoporotic effects of an antidepressant tianeptine on ovariectomized rats [S0753332216322752]
        Journal of Inorganic Biochemistry Biomimetic fabrication of antibacterial calcium phosphates mediated by polydopamine. [pubmed:29049953]
        Biomedicine & pharmacotherapy Icaritin induces MC3T3-E1 subclone14 cell differentiation through estrogen receptor-mediated ERK1/2 and p38 signaling activation. [pubmed:28742995]
        Journal of?Clinical?Periodontology? Peroxisome proliferator‐activated receptor γ plays dual roles on experimental periodontitis in rats [Pubmed:29574908]
        Macromolecular?Bioscience Strontium‐Substituted Hydroxyapatite‐Gelatin Biomimetic Scaffolds Modulate Bone Cell Response [Pubmed:29877029]
        Journal of Inorganic Biochemistry Gradient coatings of strontium hydroxyapatite/zinc β-tricalcium phosphate as a tool to modulate osteoblast/osteoclast response [Pubmed:29525694]
        DNA?and?Cell?Biology miRNA-133a-5p Inhibits the Expression of Osteoblast Differentiation-Associated Markers by Targeting the 3′ UTR of [Pubmed:29359964]
        Bone The sole and combined effect of simvastatin and platelet rich fibrin as a filling material in induced bone defect in tibia of albino rats [Pubmed: 30208342]
        Experimental Gerontology The decline of whole-body glucose metabolism in ovariectomized rats [Pubmed: 30292771]
        BMC?Complementary and Alternative?Medicine? Hepatoprotective activity of Erythrina× neillii leaf extract and characterization of its phytoconstituents [Pubmed: 28095910]
        Journal of Hematology &?Oncology Multiple myeloma-derived exosomes are enriched of amphiregulin (AREG) and activate the epidermal growth factor pathway in the bone microenvironment?… [Pubmed: 30621731]
        JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS CoCr porous scaffolds manufactured via selective laser melting in orthopedics: Topographical, mechanical, and biological characterization [Pubmed: 30689288]
        Chemico-Biological?Interactions Lead exposure inhibits osteoblastic differentiation and inactivates the canonical Wnt signal and recovery by icaritin in MC3T3-E1 subclone 14 cells [Pubmed: 30731080]
        Journal of Orthopaedic Surgery and Research Macrophage inhibits the osteogenesis of fibroblasts in ultrahigh molecular weight polyethylene (UHMWPE) wear particle-induced osteolysis []
        Bangladesh Journal of Medical Science Zn as the Factor of Alkaline Phosphatase in Periodontal Patients consuming Minangkabau food in West Sumatra, Indonesia []
        Molecular Therapy-Nucleic Acids AAV-anti-miR-214 prevents collapse of the femoral head in osteonecrosis by regulating osteoblast and osteoclast activities [Pubmed: 31739209]
        Journal of Korean Neurosurgical Society Rutin Improves Bone Histomorphometric Values by Reduction of Osteoclastic Activity in Osteoporosis Mouse Model Induced by Bilateral Ovariectomy [Pubmed: 32172552]
        Journal of Applied Biomaterials & Functional Materials Effects of tricalcium silicate/sodium alginate/calcium sulfate hemihydrate composite cements on osteogenic performances in vitro and in vivo [Pubmed: 32138579]
        Nutrients Osteoprotective Activity and Metabolite Fingerprint via UPLC/MS and GC/MS of Lepidium sativum in Ovariectomized Rats [Pubmed: 32668691]
        International Journal of Clinical and Experimental Pathology Matrilin-2 is a novel prognostic marker in osteosarcoma []
        Revista Argentina de Clínica Psicológica Research on the Mechanism of miR-142-5p's Promotion of the Function and Mineralization of Osteoblasts by Down-regulating the Expression of WWP1 Gene []
        Ceramics International Platinum nanoparticles supported on functionalized hydroxyapatite: Anti-oxidant properties and bone cells response []
        Bioelectromagnetics Static Magnetic Field (2¨C4 T) Improves Bone Microstructure and Mechanical Properties by Coordinating Osteoblast/Osteoclast Differentiation in Mice [33655538]
        PeerJ From hair to liver: emerging application of hair follicle mesenchymal stem cell transplantation reverses liver cirrhosis by blocking the TGF-β/Smad signaling?… [Pubmed:35186473]
        Cell Death Discov ECM1 modified HF-MSCs targeting HSC attenuate liver cirrhosis by inhibiting the TGF-β/Smad signaling pathway [Pubmed:35136027]
        Int J Mol Sci Antiosteoporotic Nanohydroxyapatite Zoledronate Scaffold Seeded with Bone Marrow Mesenchymal Stromal Cells for Bone Regeneration: A 3D In Vitro Model [Pubmed:35682677]
        Journal of Bionic Engineering Effect of Antibacterial Enoxacin on the Properties of Injectable Nano-hydroxyapatite/Polyurethane Cement for Bone Repairing []