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      1. 趨化素(CHEM)檢測試劑盒(酶聯免疫吸附試驗法)

        ELISA Kit for Chemerin (CHEM)

        TIG2; RARRES2; Tazarotene-Induced Gene 2; Retinoic Acid Receptor Responder 2,Tazarotene Induced; RAR-responsive protein TIG2

        • 趨化素(CHEM)檢測試劑盒(酶聯免疫吸附試驗法) 產品包裝(模擬)
        • 趨化素(CHEM)檢測試劑盒(酶聯免疫吸附試驗法) 產品包裝(模擬)
        • 趨化素(CHEM)檢測試劑盒(酶聯免疫吸附試驗法) 實驗結果圖
        • SEA945Mu.jpg 標準曲線圖
        • Certificate 通過ISO 9001、ISO 13485質量體系認證

        特異性

        本試劑盒用于檢測趨化素(CHEM),經檢測與其它相似物質無明顯交叉反應。
        由于受到技術及樣本來源的限制,不可能完成對所有相關或相似物質交叉反應檢測,因此本試劑盒有可能與未經檢測的其它物質有交叉反應。

        回收率

        分別于定值血清及血漿樣本中加入一定量的趨化素(CHEM)(加標樣品),重復測定并計算其均值,回收率為測定值與理論值的比率。

        樣本 回收率范圍(%) 平均回收率(%)
        serum(n=5) 80-94 89
        EDTA plasma(n=5) 81-95 88
        heparin plasma(n=5) 89-102 95

        精密度

        精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
        批內差:取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續測定20 次,分別計算不同濃度樣本的平均值及SD值。
        批間差:選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定,每個樣本使用同一試劑盒重復測定8次,分別計算不同濃度樣本的平均值及SD值。
        批內差: CV<10%
        批間差: CV<12%

        線性

        在定值血清及血漿樣本內加入適量的趨化素(CHEM),并倍比稀釋成1:2,1:4,1:8,1:16的待測樣本,線性范圍即為稀釋后樣本中趨化素(CHEM)含量的測定值與理論值的比率。

        樣本 1:2 1:4 1:8 1:16
        serum(n=5) 97-105% 86-104% 86-102% 95-105%
        EDTA plasma(n=5) 83-97% 95-102% 80-90% 83-97%
        heparin plasma(n=5) 89-96% 87-103% 86-101% 79-97%

        穩定性

        經測定,試劑盒在有效期內按推薦溫度保存,其活性降低率小于5%。
        為減小外部因素對試劑盒破壞前后檢測值的影響,實驗室的環境條件需盡量保持一致,尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

        實驗流程

        1. 實驗前標準品、試劑及樣本的準備;
        2. 加樣(標準品及樣本)100µL,37°C孵育1小時;
        3. 吸棄,加檢測溶液A100µL,37°C孵育1小時;
        4. 洗板3次;
        5. 加檢測溶液B100µL,37°C孵育30分鐘;
        6. 洗板5次;
        7. 加TMB底物90µL,37°C孵育10-20分鐘;
        8. 加終止液50µL,立即450nm讀數。

        實驗原理

        將趨化素(CHEM)抗體包被于96孔微孔板中,制成固相載體,向微孔中分別加入標準品或標本,其中的趨化素(CHEM)與連接于固相載體上的抗體結合,然后加入生物素化的趨化素(CHEM)抗體,將未結合的生物素化抗體洗凈后,加入HRP標記的親和素,再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉化成藍色,并在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中的趨化素(CHEM)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值),計算樣品濃度。

        相關產品

        編號 適用物種:Mus musculus (Mouse,小鼠) 應用(僅供研究使用,不用于臨床診斷!)
        RPA945Mu01 趨化素(CHEM)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
        RPA945Mu02 趨化素(CHEM)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
        PAA945Mu02 趨化素(CHEM)多克隆抗體 WB; IHC; ICC; IP.
        PAA945Mu01 趨化素(CHEM)多克隆抗體 WB; IHC; ICC; IP.
        SEA945Mu 趨化素(CHEM)檢測試劑盒(酶聯免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
        LMA945Mu 趨化素(CHEM)單因子檢測試劑盒(流式熒光發光法) Magnetic Luminex Assay for Antigen Detection.
        KSA945Mu01 趨化素(CHEM)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) Main materials for "Do It (ELISA Kit) Yourself".

        參考文獻

        雜志 參考文獻
        Scandinavian Journal of Gastroenterology Serum chemerin and vaspin in non-alcoholic fatty liver disease [PubMed: 20095887]
        Gastroenterology & Hepatology Chemerin, vaspin and insulin resistance in chronic hepatitis C [Wiley: source]
        Placenta Expression and regulation of chemerin during rat pregnancy [ScienceDirect: S0143400412000902]
        Acta Pharmacologica Sinica Chronic ethanol consumption increases the levels of chemerin in the serum and adipose tissue of humans and rats [Nature: 201211]
        RAED dergisi Ankilozan spondilitli hastalarda adipositokin düzeyleri ve insülin direnci [Raeddergisi: Source]
        Journal of Cellular and Molecular Medicine Association of chemerin with oxidative stress, inflammation and classical adipokines in non-diabetic obese patients [Pubmed: 24702860]
        Clinical Endocrinology Strong correlations between circulating chemerin levels and lipoprotein subfractions in nondiabetic obese and nonobese subjects [Pubmed: 24303851]
        Oral Dis. Impact of OSA on Biological Markers in Morbid Obesity and Metabolic Syndrome [Pubmed:24106991]
        Endocrine Chemerin plays a protective role by regulating human umbilical vein endothelial cell-induced nitric oxide signaling in preeclampsia [Pubmed:24840719]
        The Clinical Respiratory Journal Relationship between plasma chemerin levels and disease severity in COPD patients [Pubmed:24865134]
        Gynecol Endocrinol. Elevated serum chemerin in Chinese women with hyperandrogenic PCOS [Pubmed:24927079]
        Endocrine. Protective effect of liraglutide against ER stress in the liver of high-fat diet-induced insulin-resistant rats. [Pubmed:25471281]
        Obesity Surgery Long-term Effect of Ileal Transposition on Adipokine Serum Level in Zucker (Orl)-Lepr fa Fatty Rats [Pubmed:25697126]
        Dermatologica Sinica Chemerin and calprotectin levels correlate with disease activity and inflammation markers in psoriasis vulgaris [Sciencedirect:S1027811714000470]
        Dis Markers Serum Chemerin Levels in relation to Osteoporosis and Bone Mineral Density: A Case-Control Study [PubMed: 26170530]
        Med Sci Monit Association of Serum Chemerin Levels with Acute Ischemic Stroke and Carotid Artery Atherosclerosis in a Chinese Population [PubMed: 26471865]
        Endocrine Protective effect of liraglutide against ER stress in the liver of high-fat diet-induced insulin-resistant rats [PubMed: 25471281]
        Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids Effects of alpha-lipoic acid on chemerin secretion in 3T3-L1 and human adipocytes [Pubmed:26721419]
        Medicine (Baltimore). Association?of?chemerin?levels?and?bone?mineral?density?in?Chinese?obese?postmenopausalwomen. [pubmed:27583869]
        Oxidative?Medicine?and?Cellular?Longevity Changes of Plasma FABP4, CRP, Leptin, and Chemerin Levels in relation to Different Dietary Patterns and Duodenal-Jejunal Omega Switch Surgery in Sprague?… [Pubmed:29849871]
        Current?Eye?Research Chemerin is associated with diabetic retinopathy in type 2 diabetes [Pubmed:25848840]
        Journal of?Oral?Rehabilitation Expression of chemerin in the synovial fluid of patients with temporomandibular joint disorders [Pubmed:29331039]
        International?Journal?of?Cancer Epigenetic regulation of Amphiregulin and Epiregulin in colorectal cancer [Pubmed: 30252132]
        Experimental Gerontology The decline of whole-body glucose metabolism in ovariectomized rats [Pubmed: 30292771]
        Pancreatology Chemerin alleviates acute pancreatitis in the rat thorough modulation of NF-κB signal [Doi: 10.1016/j.pan.2019.02.005]
        Hypertens Pregnancy Protective effect of vitamin D supplementation in a rat modal of preeclampsia: a possible implication of chemerin [Pubmed: 30922121]
        Pancreatology Chemerin alleviates acute pancreatitis in the rat thorough modulation of NF-kB signal [Pubmed: 30833212]
        Digestion Association between High Levels of Circulating Chemerin and Colorectal Adenoma in Men [Pubmed: 31269491]
        Vitamin D Supplementation Reduces Serum Chemerin Level in Gestational Diabetes Mellitus Rat Model []
        Aging Medicine Plasma levels of adiponectin and chemerin are associated with early stage of atherosclerosis in older adults with type 2 diabetes mellitus []
        Eur J Nutr The dietary inflammatory index is associated with anti-and pro-inflammatory adipokines in Brazilian schoolchildren [33575861]
        Eur J Pediatr Pro-and anti-inflammatory adipokines are associated with cardiometabolic risk markers in Brazilian schoolchildren [33834274]
        Indian Journal of Forensic Medicine & Toxicology Altered Serum Marker of Adipokines Profile in Breast Cancer Women []
        Int J Womens Health Association of High Serum Chemerin with Bone Mineral Density Loss and Osteoporotic Fracture in Elderly Chinese Women [Pubmed:35140527]
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        亚洲精品国产情侣AV在线,日韩精品一区二区三区色欲AV,免费看高清黄A级毛片