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      1. 血管緊張素轉化酶2(ACE2)檢測試劑盒(酶聯免疫吸附試驗法)

        ELISA Kit for Angiotensin I Converting Enzyme 2 (ACE2)

        ACEH; ACEII; ACE-II; Peptidyl-Dipeptidase A; ACE-related carboxypeptidase; Angiotensin-converting enzyme homolog; Metalloprotease MPROT15

        • 血管緊張素轉化酶2(ACE2)檢測試劑盒(酶聯免疫吸附試驗法) 產品包裝(模擬)
        • 血管緊張素轉化酶2(ACE2)檢測試劑盒(酶聯免疫吸附試驗法) 產品包裝(模擬)
        • 血管緊張素轉化酶2(ACE2)檢測試劑盒(酶聯免疫吸附試驗法) 實驗結果圖
        • SEB886Hu.jpg 標準曲線圖
        • Certificate 通過ISO 9001、ISO 13485質量體系認證

        特異性

        本試劑盒用于檢測血管緊張素轉化酶2(ACE2),經檢測與其它相似物質無明顯交叉反應。
        由于受到技術及樣本來源的限制,不可能完成對所有相關或相似物質交叉反應檢測,因此本試劑盒有可能與未經檢測的其它物質有交叉反應。

        回收率

        分別于定值血清及血漿樣本中加入一定量的血管緊張素轉化酶2(ACE2)(加標樣品),重復測定并計算其均值,回收率為測定值與理論值的比率。

        樣本 回收率范圍(%) 平均回收率(%)
        serum(n=5) 94-102 98
        EDTA plasma(n=5) 93-101 97
        heparin plasma(n=5) 85-104 91

        精密度

        精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
        批內差:取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續測定20 次,分別計算不同濃度樣本的平均值及SD值。
        批間差:選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定,每個樣本使用同一試劑盒重復測定8次,分別計算不同濃度樣本的平均值及SD值。
        批內差: CV<10%
        批間差: CV<12%

        線性

        在定值血清及血漿樣本內加入適量的血管緊張素轉化酶2(ACE2),并倍比稀釋成1:2,1:4,1:8,1:16的待測樣本,線性范圍即為稀釋后樣本中血管緊張素轉化酶2(ACE2)含量的測定值與理論值的比率。

        樣本 1:2 1:4 1:8 1:16
        serum(n=5) 81-102% 78-97% 78-96% 78-104%
        EDTA plasma(n=5) 83-102% 97-105% 83-96% 82-91%
        heparin plasma(n=5) 86-94% 79-92% 85-92% 90-101%

        穩定性

        經測定,試劑盒在有效期內按推薦溫度保存,其活性降低率小于5%。
        為減小外部因素對試劑盒破壞前后檢測值的影響,實驗室的環境條件需盡量保持一致,尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

        實驗流程

        1. 實驗前標準品、試劑及樣本的準備;
        2. 加樣(標準品及樣本)100µL,37°C孵育1小時;
        3. 吸棄,加檢測溶液A100µL,37°C孵育1小時;
        4. 洗板3次;
        5. 加檢測溶液B100µL,37°C孵育30分鐘;
        6. 洗板5次;
        7. 加TMB底物90µL,37°C孵育10-20分鐘;
        8. 加終止液50µL,立即450nm讀數。

        實驗原理

        將血管緊張素轉化酶2(ACE2)抗體包被于96孔微孔板中,制成固相載體,向微孔中分別加入標準品或標本,其中的血管緊張素轉化酶2(ACE2)與連接于固相載體上的抗體結合,然后加入生物素化的血管緊張素轉化酶2(ACE2)抗體,將未結合的生物素化抗體洗凈后,加入HRP標記的親和素,再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉化成藍色,并在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中的血管緊張素轉化酶2(ACE2)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值),計算樣品濃度。

        相關產品

        編號 適用物種:Homo sapiens (Human,人) 應用(僅供研究使用,不用于臨床診斷!)
        EPB886Hu61 血管緊張素轉化酶2(ACE2)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
        APB886Hu01 血管緊張素轉化酶2(ACE2)活性蛋白 Cell?culture;?Activity?Assays.
        APB886Hu61 血管緊張素轉化酶2(ACE2)活性蛋白 Cell?culture;?Activity?Assays.
        RPB886Hu01 血管緊張素轉化酶2(ACE2)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
        RPB886Hu02 血管緊張素轉化酶2(ACE2)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
        PAB886Hu01 血管緊張素轉化酶2(ACE2)多克隆抗體 WB; IHC; ICC; IP.
        MAB886Hu21 血管緊張素轉化酶2(ACE2)單克隆抗體 WB; IHC; ICC; IP.
        MAB886Hu22 血管緊張素轉化酶2(ACE2)單克隆抗體 WB; IHC; ICC; IP.
        SEB886Hu 血管緊張素轉化酶2(ACE2)檢測試劑盒(酶聯免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
        AEB886Hu 抗血管緊張素轉化酶2抗體(Anti-ACE2)檢測試劑盒(酶聯免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antibody Detection.
        LMB886Hu 血管緊張素轉化酶2(ACE2)單因子檢測試劑盒(流式熒光發光法) Magnetic Luminex Assay for Antigen Detection.

        參考文獻

        雜志 參考文獻
        Nature Medicine Angiotensin-Converting Enzyme 2 Overexpression Remarkably Ameliorated Glomerular Injury in a Rat Model of Diabetic Nephropathy: A Comparison with ACE Inhibition [MolMed: 10_11_liu]
        Journal of Cancer Therapy Plasma Levels of Angiotensin-Converting Enzymes 1 and 2 and AGTR2 (T1247G and A5235G) Gene Polymorphisms Are Associated to Breast Cancer Progression. [Scirp: Source]
        Reprod Toxicol.? ACE2 activation by xanthenone prevents leptin-induced increases in blood pressure and proteinuria during pregnancy in Sprague-Dawley rats [Pubmed:25205467]
        Intensive Care Medicine Experimental Angiotensin converting enzymes in patients with acute respiratory distress syndrome [Content: 3]
        Journal of Chromatography A Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosis [S0021967317311858]
        Journal of Clinical Pharmacy and Therapeutics Relationship between genetic variants of ACE2 gene and circulating levels of ACE2 and its metabolites. [pubmed:28895159]
        Innate?Immunity? Lipoxin A attenuates LPS-induced acute lung injury via activation of the ACE2-Ang-(1-7)-Mas axis [Pubmed:29969931]
        Journal of?Surgical?Research Impact of angiotensin-converting enzyme 2 levels on postoperative pneumonia after esophagectomy [Pubmed:29506841]
        canadian journal of physiology and pharmacology Could cardioprotective effect of ACE2 activator “diminazene aceturate” is more potent than ACE inhibitor “Enalapril” on acute myocardial infarction in rats? [Pubmed: 30840489]
        Intensive Care Medicine Angiotensin-converting enzymes in acute respiratory distress syndrome []
        BioMed Research International AVE 0991 Attenuates Pyroptosis and Liver Damage after Heatstroke by Inhibiting the ROS-NLRP3 Inflammatory Signalling Pathway []
        Heart?and?vessels Relationship between circulating levels of angiotensin-converting enzyme 2-angiotensin-(1–7)-MAS axis and coronary heart disease [Pubmed: 31359146]
        Medicine Alteration and association between serum ACE2/angiotensin (1-7)/Mas axis and oxidative stress in chronic kidney disease: A pilot study [Pubmed: 32756181]
        American Journal of Respiratory and Critical Care Medicine Equilibrium Angiotensin Metabolite Profiling in Patients with Acute Respiratory Distress Syndrome Indicates Angiotensin Converting Enzyme Inhibition [Pubmed: 32628511]
        Clinical Research in Cardiology Determinants of soluble angiotensin-converting enzyme 2 concentrations in adult patients with complex congenital heart disease [33280062]
        Clinical & Translational Immunology Pattern of circulating SARS?\CoV?\2?\specific antibody?\secreting and memory B?\cell generation in patients with acute COVID?\19 [33552508]
        researchsquare Novel transgenic mice with Cre-dependent co-expression of GFP and human ACE2: a safe tool for study of COVID-19 pathogenesis [33855640]
        Viruses Different Neutralization Sensitivity of SARS-CoV-2 Cell-to-Cell and Cell-Free Modes of Infection to Convalescent Sera []
        Sci Rep Immunoreactivity of the SARS-CoV-2 entry proteins ACE-2 and TMPRSS-2 in murine models of hormonal manipulation, ageing, and cardiac injury [34907257]
        European Journal of Clinical Investigation Angiotensin‐converting enzyme 2 and Transmembrane protease serine 2 in female and male patients with end‐stage kidney disease [Pubmed:35366343]
        Metabolites Ramipril Reduces Acylcarnitines and Distinctly Increases Angiotensin-Converting Enzyme 2 Expression in Lungs of Rats [Pubmed:35448480]
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        亚洲精品国产情侣AV在线,日韩精品一区二区三区色欲AV,免费看高清黄A级毛片