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      1. 血管緊張素轉化酶(ACE)檢測試劑盒(酶聯免疫吸附試驗法)

        ELISA Kit for Angiotensin I Converting Enzyme (ACE)

        CD143; ACE1; DCP1; ACEI; ACE-I; Kininase II; Angiotensin-Converting Enzyme; Peptidyl-Dipeptidase A; Dipeptidyl Carboxypeptidase 1; Angiotensin-converting enzyme, soluble form

        • 血管緊張素轉化酶(ACE)檢測試劑盒(酶聯免疫吸附試驗法) 產品包裝(模擬)
        • 血管緊張素轉化酶(ACE)檢測試劑盒(酶聯免疫吸附試驗法) 產品包裝(模擬)
        • 血管緊張素轉化酶(ACE)檢測試劑盒(酶聯免疫吸附試驗法) 實驗結果圖
        • SEA004Hu.jpg 標準曲線圖
        • Certificate 通過ISO 9001、ISO 13485質量體系認證

        特異性

        本試劑盒用于檢測血管緊張素轉化酶(ACE),經檢測與其它相似物質無明顯交叉反應。
        由于受到技術及樣本來源的限制,不可能完成對所有相關或相似物質交叉反應檢測,因此本試劑盒有可能與未經檢測的其它物質有交叉反應。

        回收率

        分別于定值血清及血漿樣本中加入一定量的血管緊張素轉化酶(ACE)(加標樣品),重復測定并計算其均值,回收率為測定值與理論值的比率。

        樣本 回收率范圍(%) 平均回收率(%)
        serum(n=5) 84-94 88
        EDTA plasma(n=5) 86-93 90
        heparin plasma(n=5) 86-103 101

        精密度

        精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
        批內差:取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續測定20 次,分別計算不同濃度樣本的平均值及SD值。
        批間差:選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定,每個樣本使用同一試劑盒重復測定8次,分別計算不同濃度樣本的平均值及SD值。
        批內差: CV<10%
        批間差: CV<12%

        線性

        在定值血清及血漿樣本內加入適量的血管緊張素轉化酶(ACE),并倍比稀釋成1:2,1:4,1:8,1:16的待測樣本,線性范圍即為稀釋后樣本中血管緊張素轉化酶(ACE)含量的測定值與理論值的比率。

        樣本 1:2 1:4 1:8 1:16
        serum(n=5) 99-105% 87-94% 78-101% 80-91%
        EDTA plasma(n=5) 94-102% 89-98% 98-105% 78-94%
        heparin plasma(n=5) 84-95% 90-97% 99-105% 93-105%

        穩定性

        經測定,試劑盒在有效期內按推薦溫度保存,其活性降低率小于5%。
        為減小外部因素對試劑盒破壞前后檢測值的影響,實驗室的環境條件需盡量保持一致,尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

        實驗流程

        1. 實驗前標準品、試劑及樣本的準備;
        2. 加樣(標準品及樣本)100µL,37°C孵育1小時;
        3. 吸棄,加檢測溶液A100µL,37°C孵育1小時;
        4. 洗板3次;
        5. 加檢測溶液B100µL,37°C孵育30分鐘;
        6. 洗板5次;
        7. 加TMB底物90µL,37°C孵育10-20分鐘;
        8. 加終止液50µL,立即450nm讀數。

        實驗原理

        將血管緊張素轉化酶(ACE)抗體包被于96孔微孔板中,制成固相載體,向微孔中分別加入標準品或標本,其中的血管緊張素轉化酶(ACE)與連接于固相載體上的抗體結合,然后加入生物素化的血管緊張素轉化酶(ACE)抗體,將未結合的生物素化抗體洗凈后,加入HRP標記的親和素,再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉化成藍色,并在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中的血管緊張素轉化酶(ACE)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值),計算樣品濃度。

        相關產品

        編號 適用物種:Homo sapiens (Human,人) 應用(僅供研究使用,不用于臨床診斷!)
        RPA004Hu01 血管緊張素轉化酶(ACE)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
        RPA004Hu02 血管緊張素轉化酶(ACE)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
        APA004Hu02 血管緊張素轉化酶(ACE)活性蛋白 Cell?culture;?Activity?Assays.
        PAA004Hu01 血管緊張素轉化酶(ACE)多克隆抗體 WB; IHC; ICC; IP.
        PAA004Hu02 血管緊張素轉化酶(ACE)多克隆抗體 WB; ICC; IHC-P; IHC-F; ELISA.
        LAA004Hu81 血管緊張素轉化酶(ACE)多克隆抗體(異硫氰酸熒光素標記) WB; IHC; ICC; IF.
        LAA004Hu71 血管緊張素轉化酶(ACE)多克隆抗體(生物素標記) WB; IHC; ICC.
        MAA004Hu22 血管緊張素轉化酶(ACE)單克隆抗體 WB; IHC; ICC; IP.
        SEA004Hu 血管緊張素轉化酶(ACE)檢測試劑盒(酶聯免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
        SCA004Hu 血管緊張素轉化酶(ACE)檢測試劑盒(化學發光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
        LMA004Hu 血管緊張素轉化酶(ACE)單因子檢測試劑盒(流式熒光發光法) Magnetic Luminex Assay for Antigen Detection.

        參考文獻

        雜志 參考文獻
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        Journal of Cancer Therapy Plasma Levels of Angiotensin-Converting Enzymes 1 and 2 and AGTR2 (T1247G and A5235G) Gene Polymorphisms Are Associated to Breast Cancer Progression. [Scirp: Source]
        The American journal of clinical nutrition Vascular function and atherosclerosis progression after 1 y of flavonoid intake in statin-treated postmenopausal women with type 2 diabetes: a double-blind randomized controlled trial. [Pubmed: 23553151]
        Journal of Vascular Surgery Cathepsin G deficiency reduces periaortic calcium chloride injury-induced abdominal aortic aneurysms in mice [Pubmed:25037606]
        Reprod Toxicol.? ACE2 activation by xanthenone prevents leptin-induced increases in blood pressure and proteinuria during pregnancy in Sprague-Dawley rats [Pubmed:25205467]
        Chemical Research in Toxicology Sub-chronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwall Carbon Nanotubes by Intratracheal Instillation [Pubmed:25580880]
        Chem Biol Interact Arsenic causes aortic dysfunction and systemic hypertension in rats: Augmentation of angiotensin II signaling [PubMed: 26079204]
        Biomed Res Int Protective Effect of Enalapril against Methionine-Enriched Diet-Induced Hypertension: Role of Endoplasmic Reticulum and Oxidative Stress [PubMed: 26640794]
        Clinics (Sao Paulo) Mechanisms [PubMed: 26602523]
        Chemical Research in Toxicology Subchronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwalled Carbon Nanotubes by Intratracheal Instillation [PubMed: 25580880]
        Intensive Care Medicine Experimental Angiotensin converting enzymes in patients with acute respiratory distress syndrome [Content: 3]
        Evid Based Complement Alternat Med. Garlic Attenuates Plasma and Kidney ACE-1 and AngII Modulations in Early Streptozotocin-Induced Diabetic Rats: Renal Clearance and Blood Pressure Implications [Pubmed:27293465]
        Вавиловский журнал генетики и селекции Ренин-ангиотензин-альдостероновая система у крыс линии НИСАГ (ISIAH) со стресс-индуцированной артериальной гипертензией [30_Dubinina.pdf]
        Animal Biology The effect of heat stress on bull sperm quality and related HSPs expression [content:journals]
        biomedicine & pharmacotherapy Citrus leaf extract reduces blood pressure and vascular damage in repeatedly heated palm oil diet-Induced hypertensive rats. [pubmed:28068636]
        Biomedicine & Pharmacotherapy Parkia speciosa empty pod prevents hypertension and cardiac damage in rats given N(G)-nitro- L -arginine methyl ester [pubmed:28992471]
        Journal of Functional Foods Antihypertensive effects of?Ocimum gratissimumextract: Angiotensin-converting enzyme inhibitor in vitro and in vivo investigation [10.1016/j.jff.2017.05.033]
        Molecules Protective Effect of Polyphenol-Rich Extract from Bee Pollen in a High-Fat Diet [Pubmed:29614743]
        Journal of?Surgical?Research Impact of angiotensin-converting enzyme 2 levels on postoperative pneumonia after esophagectomy [Pubmed:29506841]
        Intensive Care Medicine Angiotensin-converting enzymes in acute respiratory distress syndrome []
        International Journal of Biological Sciences Vascular Protection of TPE-CA on Hyperhomocysteinemia-induced Vascular Endothelial Dysfunction Through AA Metabolism Modulated CYPs Pathway []
        Medicine Alteration and association between serum ACE2/angiotensin (1-7)/Mas axis and oxidative stress in chronic kidney disease: A pilot study [Pubmed: 32756181]
        American Journal of Respiratory and Critical Care Medicine Equilibrium Angiotensin Metabolite Profiling in Patients with Acute Respiratory Distress Syndrome Indicates Angiotensin Converting Enzyme Inhibition [Pubmed: 32628511]
        Int Immunopharmacol Crocin protects against cardiotoxicity induced by doxorubicin through TLR-2/NF-κB signal pathway in vivo and vitro [Pubmed: 32388215]
        Food Bioscience Djulis (Chenopodium formosanum) and its bioactive compounds affect vasodilation, angiotensin converting enzyme activity, and hypertension []
        Journal of Extracellular Vesicles MiR155-5p in adventitial fibroblasts-derived extracellular vesicles inhibits vascular smooth muscle cell proliferation via suppressing angiotensin-converting enzyme?… []
        Biotechnology and Pharmacy Chrysin pretreatment improves mitochondrial enzymes and angiotensin converting enzymes in L-NAME induced hypertensive rats []
        Dikkat Eksikli?i Hiperaktivite Bozuklu?u Tan?s? Olan Anjiyotensin I, II, III, Alamandin peptid, Anjiyotensin D?nü?türücü Enzim (ACE), NADPH Oksidaz Enzim Serum?… []
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        亚洲精品国产情侣AV在线,日韩精品一区二区三区色欲AV,免费看高清黄A级毛片