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      1. 補體成分3(C3)檢測試劑盒(酶聯免疫吸附試驗法)

        ELISA Kit for Complement Component 3 (C3)

        ASP; CPAMD1; Complement Protein C3; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1; C3a anaphylatoxin; Acylation stimulating protein

        • 補體成分3(C3)檢測試劑盒(酶聯免疫吸附試驗法) 產品包裝(模擬)
        • 補體成分3(C3)檢測試劑盒(酶聯免疫吸附試驗法) 產品包裝(模擬)
        • 補體成分3(C3)檢測試劑盒(酶聯免疫吸附試驗法) 實驗結果圖
        • SEA861Hu.jpg 標準曲線圖
        • Certificate 通過ISO 9001、ISO 13485質量體系認證

        特異性

        本試劑盒用于檢測補體成分3(C3),經檢測與其它相似物質無明顯交叉反應。
        由于受到技術及樣本來源的限制,不可能完成對所有相關或相似物質交叉反應檢測,因此本試劑盒有可能與未經檢測的其它物質有交叉反應。

        回收率

        分別于定值血清及血漿樣本中加入一定量的補體成分3(C3)(加標樣品),重復測定并計算其均值,回收率為測定值與理論值的比率。

        樣本 回收率范圍(%) 平均回收率(%)
        serum(n=5) 78-92 88
        EDTA plasma(n=5) 87-102 92
        heparin plasma(n=5) 93-101 97

        精密度

        精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
        批內差:取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續測定20 次,分別計算不同濃度樣本的平均值及SD值。
        批間差:選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定,每個樣本使用同一試劑盒重復測定8次,分別計算不同濃度樣本的平均值及SD值。
        批內差: CV<10%
        批間差: CV<12%

        線性

        在定值血清及血漿樣本內加入適量的補體成分3(C3),并倍比稀釋成1:2,1:4,1:8,1:16的待測樣本,線性范圍即為稀釋后樣本中補體成分3(C3)含量的測定值與理論值的比率。

        樣本 1:2 1:4 1:8 1:16
        serum(n=5) 90-98% 81-98% 91-98% 95-105%
        EDTA plasma(n=5) 91-105% 89-98% 99-105% 88-102%
        heparin plasma(n=5) 79-93% 89-96% 89-96% 78-92%

        穩定性

        經測定,試劑盒在有效期內按推薦溫度保存,其活性降低率小于5%。
        為減小外部因素對試劑盒破壞前后檢測值的影響,實驗室的環境條件需盡量保持一致,尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

        實驗流程

        1. 實驗前標準品、試劑及樣本的準備;
        2. 加樣(標準品及樣本)100µL,37°C孵育1小時;
        3. 吸棄,加檢測溶液A100µL,37°C孵育1小時;
        4. 洗板3次;
        5. 加檢測溶液B100µL,37°C孵育30分鐘;
        6. 洗板5次;
        7. 加TMB底物90µL,37°C孵育10-20分鐘;
        8. 加終止液50µL,立即450nm讀數。

        實驗原理

        將補體成分3(C3)抗體包被于96孔微孔板中,制成固相載體,向微孔中分別加入標準品或標本,其中的補體成分3(C3)與連接于固相載體上的抗體結合,然后加入生物素化的補體成分3(C3)抗體,將未結合的生物素化抗體洗凈后,加入HRP標記的親和素,再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉化成藍色,并在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中的補體成分3(C3)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值),計算樣品濃度。

        相關產品

        編號 適用物種:Homo sapiens (Human,人) 應用(僅供研究使用,不用于臨床診斷!)
        RPA861Hu01 補體成分3(C3)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
        PAA861Hu01 補體成分3(C3)多克隆抗體 WB; IHC; ICC; IP.
        LAA861Hu81 補體成分3(C3)多克隆抗體(異硫氰酸熒光素標記) WB; IHC; ICC; IF.
        LAA861Hu71 補體成分3(C3)多克隆抗體(生物素標記) WB; IHC; ICC.
        MAA861Hu22 補體成分3(C3)單克隆抗體 WB; IHC; ICC; IP.
        SEA861Hu 補體成分3(C3)檢測試劑盒(酶聯免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
        LMA861Hu 補體成分3(C3)單因子檢測試劑盒(流式熒光發光法) Magnetic Luminex Assay for Antigen Detection.

        參考文獻

        雜志 參考文獻
        Artificial Organs Experimental Research With Synthetic Copolymer-Coated Cardiopulmonary Bypass Circuits: Inflammatory and Thrombogenicity Analysis [Wiley: source]
        Journal of Clinical Immunology Complement activation contributes to the injury and outcome of kidney in human anti-glomerular basement membrane disease. [PubMed: 22941511]
        Lipids in Health and Disease Beneficial effects of omega-3 fatty acids in the proteome of high-density lipoprotein proteome [PubMed: PMC3480889]
        Am J Reprod Immunol Human IgG Antinuclear Antibodies Induce Pregnancy Loss in Mice by Increasing Immune Complex Deposition in Placental Tissue: In Vivo Study [PubMed: 26388133]
        Veterinary Immunology and Immunopathology Oral administration of heat-inactivated Mycobacterium bovis reduces the response of farmed red deer to avian and bovine tuberculin [Pubmed:27032499]
        International Journal of Molecular Sciences Identification of Serum Peptidome Signatures of Non-Small Cell Lung Cancer [Pubmed:27043541]
        Food Chem. Comparative proteomics of milk fat globule membrane in goat colostrum and mature milk [Pubmed:27173528]
        Scientific Reports Generation of complement protein C3 deficient pigs by CRISPR/Cas9-mediated gene targeting. [pubmed:28694465]
        Veterinary Microbiology The response of red deer to oral administration of heat-inactivated Mycobacterium bovis and challenge with a field strain. [pubmed:28888638]
        Proteomics?Clinical?Applications iTRAQ‐Based Proteomic Analysis Reveals Protein Profile in Plasma from Children with Autism [Pubmed:29274201]
        Nanomaterials An OMV-Based Nanovaccine Confers Safety and Protection against Pathogenic Escherichia coli via Both Humoral and Predominantly Th1 Immune Responses in?… [Pubmed: 33233490]
        Diagnostics Systemic Alterations of Immune Response-Related Proteins during Glaucoma Development in the Murine Model DBA/2J [Pubmed: 32585848]
        Scientific Reports The bovine cumulus proteome is influenced by maturation condition and maturational competence of the oocyte [Pubmed: 32555221]
        Int Immunopharmacol Complement component 3 prevents imiquimod-induced psoriatic skin inflammation by inhibiting apoptosis in mice [Pubmed: 32535539]
        Italian Journal of Animal Science Effects of chitooligosaccharide supplementation on laying performance, egg quality, blood biochemistry, antioxidant capacity and immunity of laying hens during the?… []
        Dev Comp Immunol A deep-sea pathogenic Bacillus subtilis isolate employs different strategies to escape the killing of teleost and murine complements [33545212]
        Animal Effect of alfalfa hay and starter feed supplementation on caecal microbiota and fermentation, growth, and health of yak calves [33610512]
        British Journal of Pharmacology The suppression of Brd4 inhibits peripheral plasma cell differentiation and exhibits therapeutic potential for systemic lupus erythematosus []
        Scientific Reports Maternal Serum Proteomic Profiles of Pregnant Women With Type 1 Diabetes [34940989]
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