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      1. 鐵蛋白(FE)檢測試劑盒(酶聯免疫吸附試驗法)

        ELISA Kit for Ferritin (FE)

        • 鐵蛋白(FE)檢測試劑盒(酶聯免疫吸附試驗法) 產品包裝(模擬)
        • 鐵蛋白(FE)檢測試劑盒(酶聯免疫吸附試驗法) 產品包裝(模擬)
        • 鐵蛋白(FE)檢測試劑盒(酶聯免疫吸附試驗法) 實驗結果圖
        • SEA518Hu.jpg 標準曲線圖
        • Certificate 通過ISO 9001、ISO 13485質量體系認證

        特異性

        本試劑盒用于檢測鐵蛋白(FE),經檢測與其它相似物質無明顯交叉反應。
        由于受到技術及樣本來源的限制,不可能完成對所有相關或相似物質交叉反應檢測,因此本試劑盒有可能與未經檢測的其它物質有交叉反應。

        回收率

        分別于定值血清及血漿樣本中加入一定量的鐵蛋白(FE)(加標樣品),重復測定并計算其均值,回收率為測定值與理論值的比率。

        樣本 回收率范圍(%) 平均回收率(%)
        serum(n=5) 79-97 90
        EDTA plasma(n=5) 83-99 93
        heparin plasma(n=5) 83-102 95

        精密度

        精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
        批內差:取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續測定20 次,分別計算不同濃度樣本的平均值及SD值。
        批間差:選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定,每個樣本使用同一試劑盒重復測定8次,分別計算不同濃度樣本的平均值及SD值。
        批內差: CV<10%
        批間差: CV<12%

        線性

        在定值血清及血漿樣本內加入適量的鐵蛋白(FE),并倍比稀釋成1:2,1:4,1:8,1:16的待測樣本,線性范圍即為稀釋后樣本中鐵蛋白(FE)含量的測定值與理論值的比率。

        樣本 1:2 1:4 1:8 1:16
        serum(n=5) 78-96% 96-103% 91-103% 87-99%
        EDTA plasma(n=5) 89-102% 78-88% 93-101% 79-93%
        heparin plasma(n=5) 90-101% 94-105% 88-96% 97-104%

        穩定性

        經測定,試劑盒在有效期內按推薦溫度保存,其活性降低率小于5%。
        為減小外部因素對試劑盒破壞前后檢測值的影響,實驗室的環境條件需盡量保持一致,尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

        實驗流程

        1. 實驗前標準品、試劑及樣本的準備;
        2. 加樣(標準品及樣本)100µL,37°C孵育1小時;
        3. 吸棄,加檢測溶液A100µL,37°C孵育1小時;
        4. 洗板3次;
        5. 加檢測溶液B100µL,37°C孵育30分鐘;
        6. 洗板5次;
        7. 加TMB底物90µL,37°C孵育10-20分鐘;
        8. 加終止液50µL,立即450nm讀數。

        實驗原理

        將鐵蛋白(FE)抗體包被于96孔微孔板中,制成固相載體,向微孔中分別加入標準品或標本,其中的鐵蛋白(FE)與連接于固相載體上的抗體結合,然后加入生物素化的鐵蛋白(FE)抗體,將未結合的生物素化抗體洗凈后,加入HRP標記的親和素,再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉化成藍色,并在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中的鐵蛋白(FE)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值),計算樣品濃度。

        相關產品

        編號 適用物種:Homo sapiens (Human,人) 應用(僅供研究使用,不用于臨床診斷!)
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        SEA518Hu 鐵蛋白(FE)檢測試劑盒(酶聯免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
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        LMA518Hu 鐵蛋白(FE)單因子檢測試劑盒(流式熒光發光法) Magnetic Luminex Assay for Antigen Detection.
        KSA518Hu01 鐵蛋白(FE)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) Main materials for "Do It (ELISA Kit) Yourself".

        參考文獻

        雜志 參考文獻
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        experimental oncology Redox-regulation of gelatinases during growth of cisplatin-sensitive and resistant Guerin carcinoma. [PubMed: 25804229]
        J Cancer Sci Ther Host H68D Genotype Affects Tumor Growth in Mouse Melanoma [Open-Access: 1948-5956-1000353]
        Food Funct The effects of polysaccharides from the root of Angelica sinensis on tumor growth and iron metabolism in H22-bearing mice [Pubmed:26757699]
        Physiological research The Labile Iron Pool in Monocytes Reflects the Activity of the Atherosclerotic Process in Men with Chronic Cardiovascular Disease [pubmed:27782743]
        Expert?Review?of Hematology 4 (th) Mediterranean Multidisciplinary Course on Iron Anemia April 29 (th)-30 (th) 2016, Madrid, Spain. [pubmed:27615544]
        Oxidative Medicine and Cellular Longevity Acidic Polysaccharide from Angelica sinensis Reverses Anemia of Chronic Disease Involving the Suppression of Inflammatory Hepcidin and NF-κB Activation [pubmed:29147463]
        European Journal of Pharmaceutical Sciences sucrosomial? iron absorption studied by in vitro and ex-vivo models [pubmed:29055735]
        Journal of Food Science and Technology In vitro assessment of bio-augmented minerals from peanut oil cakes fermented by Aspergillus oryzae through Caco-2 cells. [pubmed:29051659]
        International Journal of Cuban Health & Medicine Anemia and Iron Deficiency Related to Inflammation, Helicobacter pylori Infection and Adiposity in Reproductive-age Cuban Women [10.1590/MEDICC.2017.1902030004?]
        Oman Medical Journal The Role of Insulin Therapy in Correcting Hepcidin Levels in Patients with Type 2 Diabetes Mellitus. [pubmed:28584599]
        Food Bioscience Preparation of lactose-iron complex and its cyto-toxicity, in-vitro digestion and bioaccessibility in Caco-2 cell model system [10.1016/j.fbio.2017.10.001]
        Nutrients. Long-Term Dexamethasone Exposure Down-Regulates Hepatic TFR1 and Reduces Liver Iron Concentration in Rats [pubmed:28629118]
        Food?Chemistry Physicochemical characterization of mineral (iron/zinc) bound caseinate and their mineral uptake in Caco-2 cells [Pubmed:29622185]
        Animal Feed Science and Technology Super-dosing phytase improves the growth performance of weaner pigs fed a low iron diet [10.1016:j.anifeedsci.2018.01.012]
        American Journal of Hematology Iron homeostasis in pregnancy and spontaneous abortion [Pubmed: 30394565]
        The Nigerian postgraduate medical journal the official publication of the National Postgraduate Medical College of Nigeria Assessment of iron deficiency anaemia and its risk factors among adults with chronic kidney disease in a tertiary hospital in Nigeria []
        Haematologica New thiazolidinones reduce iron overload in mouse models of hereditary hemochromatosis and β-thalassemia [Pubmed: 30792208]
        博士論文 PHYSIOLOGICAL ADAPTATIONS TO HEAT ACCLIMATION; REPERCUSSIONS ON CYCLING PERFORMANCE []
        Synthesis, characterization and cellular mineral absorption of nanoemulsions of Rhododendron arboreum flower extracts stabilized with gum arabic []
        Cell Research Transferrin plays a central role in coagulation balance by interacting with clotting factors [Pubmed: 31811276]
        international journal of biological macromolecules Gum arabic capped copper nanoparticles: Synthesis, characterization, and applications [Pubmed: 31904465]
        journal of the american college of nutrition Improvement of Mineral Absorption and Nutritional Properties of Citrullus vulgaris Seeds Using Solid-State Fermentation [Pubmed: 32255407]
        LWT-FOOD SCIENCE AND TECHNOLOGY Whey protein-iron or zinc complexation decreases pro-oxidant activity of iron and increases iron and zinc bioavailability []
        J Mol Cell Cardiol Systemic iron deficiency does not affect the cardiac iron content and progression of heart failure [34139233]
        European Journal of Pharmacology The mechanism of the imbalance between proliferation and ferroptosis in pulmonary artery smooth muscle cells based on the activation of SLC7A11 [Pubmed:35700835]
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        亚洲精品国产情侣AV在线,日韩精品一区二区三区色欲AV,免费看高清黄A级毛片