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      1. 促卵泡素(FSH)檢測試劑盒(酶聯免疫吸附試驗法)

        ELISA Kit for Follicle Stimulating Hormone (FSH)

        • 促卵泡素(FSH)檢測試劑盒(酶聯免疫吸附試驗法) 產品包裝(模擬)
        • 促卵泡素(FSH)檢測試劑盒(酶聯免疫吸附試驗法) 產品包裝(模擬)
        • 促卵泡素(FSH)檢測試劑盒(酶聯免疫吸附試驗法) 實驗結果圖
        • SEA830Hu.jpg 標準曲線圖
        • Certificate 通過ISO 9001、ISO 13485質量體系認證

        特異性

        本試劑盒用于檢測促卵泡素(FSH),經檢測與其它相似物質無明顯交叉反應。
        由于受到技術及樣本來源的限制,不可能完成對所有相關或相似物質交叉反應檢測,因此本試劑盒有可能與未經檢測的其它物質有交叉反應。

        回收率

        分別于定值血清及血漿樣本中加入一定量的促卵泡素(FSH)(加標樣品),重復測定并計算其均值,回收率為測定值與理論值的比率。

        樣本 回收率范圍(%) 平均回收率(%)
        serum(n=5) 84-102 89
        EDTA plasma(n=5) 92-101 95
        heparin plasma(n=5) 78-93 87

        精密度

        精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
        批內差:取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續測定20 次,分別計算不同濃度樣本的平均值及SD值。
        批間差:選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定,每個樣本使用同一試劑盒重復測定8次,分別計算不同濃度樣本的平均值及SD值。
        批內差: CV<10%
        批間差: CV<12%

        線性

        在定值血清及血漿樣本內加入適量的促卵泡素(FSH),并倍比稀釋成1:2,1:4,1:8,1:16的待測樣本,線性范圍即為稀釋后樣本中促卵泡素(FSH)含量的測定值與理論值的比率。

        樣本 1:2 1:4 1:8 1:16
        serum(n=5) 88-101% 82-89% 80-99% 98-105%
        EDTA plasma(n=5) 93-101% 88-105% 94-102% 93-105%
        heparin plasma(n=5) 94-101% 99-105% 83-104% 89-96%

        穩定性

        經測定,試劑盒在有效期內按推薦溫度保存,其活性降低率小于5%。
        為減小外部因素對試劑盒破壞前后檢測值的影響,實驗室的環境條件需盡量保持一致,尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

        實驗流程

        1. 實驗前標準品、試劑及樣本的準備;
        2. 加樣(標準品及樣本)100µL,37°C孵育1小時;
        3. 吸棄,加檢測溶液A100µL,37°C孵育1小時;
        4. 洗板3次;
        5. 加檢測溶液B100µL,37°C孵育30分鐘;
        6. 洗板5次;
        7. 加TMB底物90µL,37°C孵育10-20分鐘;
        8. 加終止液50µL,立即450nm讀數。

        實驗原理

        將促卵泡素(FSH)抗體包被于96孔微孔板中,制成固相載體,向微孔中分別加入標準品或標本,其中的促卵泡素(FSH)與連接于固相載體上的抗體結合,然后加入生物素化的促卵泡素(FSH)抗體,將未結合的生物素化抗體洗凈后,加入HRP標記的親和素,再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉化成藍色,并在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中的促卵泡素(FSH)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值),計算樣品濃度。

        相關產品

        編號 適用物種:Homo sapiens (Human,人) 應用(僅供研究使用,不用于臨床診斷!)
        RPA830Hu01 促卵泡素(FSH)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
        PAA830Hu01 促卵泡素(FSH)多克隆抗體 WB; IHC; ICC; IP.
        MAA830Hu21 促卵泡素(FSH)單克隆抗體 WB; IHC; ICC; IP.
        SEA830Hu 促卵泡素(FSH)檢測試劑盒(酶聯免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
        IEA830Hu 促卵泡素(FSH)檢測試劑盒(酶聯免疫吸附試驗法,快捷型) Enzyme-linked immunosorbent assay for Antigen Detection.
        CEA830Hu 促卵泡素(FSH)檢測試劑盒(酶聯免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
        LMA830Hu 促卵泡素(FSH)單因子檢測試劑盒(流式熒光發光法) Magnetic Luminex Assay for Antigen Detection.
        PGA830Hu01 促卵泡素(FSH)引物對 PCR

        參考文獻

        雜志 參考文獻
        International Journal of Clinical and Experimental Medicine Studies of the pharmacology of 17α-ethynyl-androst-5-ene-3β,7β,17β-triol, a synthetic anti-inflammatory androstene [Ijcem: IJCEM1102004]
        Science Alert Reproductive Toxicity of Tobacco Shisha Smoking on Semen Parameters and Hormones Levels among Adult Egyptian Men [SciAlert: source]
        EXCLI Journal Protective role of Hibiscus sabdariffa calyx extract against streptozotocin induced sperm damage in diabetic rats [Excli: Source]
        International Journal of Molecular and Cellular Medicine Central effects of camphor on GnRH and sexual hormones in male rat [Pubmed: 24551777]
        Urologia internationalis Effects of Tadalafil on Hemorrhagic Cystitis and Testicular Dysfunction Induced by Cyclophosphamide in Rats [Pubmed: 24051982]
        Electromagnetic Biology and Medicine The effects of long-term exposure to a 2450 MHz electromagnetic field on growth and pubertal development in female Wistar rats [Informahealthcare: Source]
        Academic Sciences Piper longum hexane fraction induces infertility by modulation of inflammatory mediators and gonadotropin insufficiency in female rats [Ijppsjournal: Source]
        Mol Cell Endocrinol. Quercetin supplementation restores testicular function and augments germ cell survival in the estrogenized rats [Pubmed:24316378]
        Endocrinology FoxO1 Is a Negative Regulator of FSHβ Gene Expression in Basal and GnRH-Stimulated Conditions in Female [Pubmed:24437485]
        Andrologia Protective effects of Eugenia jambolana extract versus N-acetyl cysteine against cisplatin-induced damage in rat testis [Pubmed:24576220]
        Environmental Toxicology and Pharmacology Endocrine disrupting effects of dichlorodiphenyltrichloroethane analogues on gonadotropin hormones in pituitary gonadotrope cells [Pubmed:24814263]
        Environmental Research Perfluorooctane sulfonate effects on the reproductive axis in adult male rats [Pubmed:25171141]
        Genet Mol Biol. Targeted disruption of the mouse testis-enriched gene Znf230 does not affect spermatogenesis or fertility [Pubmed:25505846]
        Turkish Journal of Urology Apoptosis in testicular tissue of rats after vasectomy: evaluation of eNOS, iNOS immunoreactivities and the effects of ozone therapy [Turkishjournalofurology:Source]
        oncology research Mechanistic Study on Triptorelin Action in Protecting From 5-FU-Induced Ovarian Damage in Rats [PubMed: 26629940]
        Andrologia Protective effects of Eugenia jambolana extract versus N‐acetyl cysteine against cisplatin‐induced damage in rat testis [PubMed: 24576220]
        European Journal of Pharmacology The effect of R-(-)-deprenyl administration on reproductive parameters of rat males [PubMed: 25725114]
        Andrologia Dose‐and time‐dependent effects of Garcinia kola seed extract on sexual behaviour and reproductive parameters in male Wistar rats [PubMed: 26123866]
        Reprod Toxicol Simvastatin decreases steroid production in the H295R cell line and decreases steroids and FSH in female rats [PubMed: 26476359]
        The Journal of The Society for Reproduction and Fertility Sex-specific prenatal stress effects on the rat reproductive axis and adrenal gland structure [Pubmed:27026714]
        Biology of Reproduction Maternal High-Fat Diet Induced Loss of Fetal Oocytes Is Associated with Compromised Follicle Growth in Adult Rat Offspring [Pubmed:26962114]
        Andrologia Dose- and time-dependent?effects?of?Garcinia kola?seed?extract?on?sexual behaviour?and?reproductiveparameters?in?male Wistar rats. [Pubmed:26123866]
        Scientific Reports Molecular characterization of insulin resistance and glycolytic metabolism in the rat uterus [pubmed:27461373]
        33 Metformin Ameliorates Uterine Defects in a Rat Model of Polycystic Ovary Syndrome. [pubmed:28336389]
        environmental pollution Environmental exposure to polycyclic aromatic hydrocarbons (PAHs): The correlation with and impact on reproductive hormones in umbilical cord serum. [pubmed:27838061]
        Andrologia Effects?of?treatment?with?Hypoxis?hemerocallidea?extract?on?sexual?behaviour?and?reproductiveparameters?in?male?rats. [pubmed:28000943]
        Biology?of?Reproduction Maternal High-Fat Diet-Induced Loss of Fetal Oocytes Is Associated with Compromised Follicle Growth in Adult Rat Offspring. [pubmed:26962114]
        J Endocrinol. Impact of uteroplacental insufficiency on postnatal rat male gonad. [pubmed:27885054]
        Int J Pharm. New gonadotropin-releasing hormone glycolipids with direct antiproliferative activity and gonadotropin-releasing potency. [pubmed:28232269]
        Environmental Science & Technology Pyrethroid Insecticide Cypermethrin Accelerates Pubertal Onset in Male Mice via Disrupting Hypothalamic-Pituitary-Gonadal Axis [pubmed:28731686]
        Journal of Medicinal Chemistry Development of New Gonadotropin-Releasing Hormone-Modified Dendrimer Platforms with Direct Antiproliferative and Gonadotropin Releasing Activity [pubmed:28958147]
        Society of Toxicology Pyrethroid Insecticide Cypermethrin Modulates Gonadotropin Synthesis via Calcium Homeostasis and ERK1/2 Signaling in LbT2 Mouse Pituitary Cells [pubmed:29149324]
        Reproductive Toxicology Vitamin D deficiency impairs testicular development and spermatogenesis in mice [pubmed:28655646]
        The Journal of Biological Chemistry. Lysophosphatidic acid acyltransferase 3 tunes the membrane status of germ cells by incorporating docosahexaenoic acid during spermatogenesis. [pubmed:28578315]
        The Journal of Toxicological Sciences High sensitivity of testicular function to titanium nanoparticles [pubmed:28496042]
        Journal of Orthopaedic Research The Combination of icariin and constrained dynamic loading stimulation attenuates bone loss in ovariectomy‐induced osteoporotic mice [pubmed:29058779]
        DARU Journal of Pharmaceutical Sciences Beneficial effects of Heqi san on rat model of polycystic ovary syndrome through the PI3K/AKT pathway [pubmed:29020999]
        Reproduction. Effect of controlled ovarian hyperstimulation on puberty and estrus in mice offspring [pubmed:28687593]
        Annals of Rheumatic Diseases? FRI0390 Impaired ovarian reserve in patients affected by systemic lupus erythematosus: a case-control study [Suppl_2:728.1.abstract]
        Stem?Cell?Research?&?Therapy The role of mesenchymal stem cells in chemotherapy-induced gonadotoxicity [Pubmed:30021657]
        Environmental Pollution Prenatal exposure to polychlorinated biphenyl and umbilical cord hormones and birth outcomes in an island population [Pubmed:29525625]
        Frontiers in Molecular Neuroscience Impact of Triclosan on Female Reproduction through Reducing Thyroid Hormones to Suppress Hypothalamic Kisspeptin Neurons in Mice [Pubmed:29403355]
        Biology of Reproduction Maternal nutrient restriction impairs young adult offspring ovarian signaling resulting in reproductive dysfunction and follicle loss [Pubmed:29351580]
        Journal of?Ovarian?Research DHEA-induced ovarian hyperfibrosis is mediated by TGF-β signaling pathway [Pubmed:29321035]
        Medical Science Monitor Eicosapentaenoic Acid Improves Polycystic Ovary Syndrome in Rats via Sterol Regulatory Element-Binding Protein 1 (SREBP-1)/Toll-Like Receptor 4 (TLR4)?… [Pubmed:29627845]
        Human & Experimental Toxicology Palladium nanoparticle effects on endocrine reproductive system of female rats [Pubmed:29405765]
        A Pex13 knockout in germ cells induces a spermatogenic arrest [volltexte:2018/8921/]
        Biomedicine & Pharmacotherapy Crocetin attenuates DHT-induced polycystic ovary syndrome in mice via revising kisspeptin neurons [Doi: 10.1016/j.biopha.2018.08.135]
        Stem?Cells?Translational?Medicine Protective Effects of Uncultured Adipose‐Derived Stromal Vascular Fraction on Testicular Injury Induced by Torsion‐Detorsion in Rats [Pubmed: 30569668]
        Journal of Endocrinology Uterine progesterone signaling is a target for metformin therapy in polycystic ovary syndrome [Pubmed: 29535146]
        Experimental and Therapeutic Medicine Tanshinone IIA attenuates estradiol?induced polycystic ovarian syndrome in mice by ameliorating FSHR expression in the ovary []
        Biomedicine & Pharmacotherapy Protective properties of glycogen synthase kinase-3 inhibition against doxorubicin-induced oxidative damage to mouse ovarian reserve []
        Biology of Reproduction G-CSF-mobilized Peripheral Blood Mononuclear Cells Combined with Platelet-rich Plasma Accelerates Restoration of Ovarian Function in Cyclophosphamide?… [Pubmed: 31034039]
        International Journal of Reproductive Biomedicine The therapeutic potential of amifostine on cyclophosphamide-induced testicular dysfunction in rats: An experimental study. []
        Reproductive Biology and Endocrinology Menstrual blood derived mesenchymal stem cells combined with Bushen Tiaochong recipe improved chemotherapy-induced premature ovarian failure in?… [Pubmed: 31311554]
        Andrologia Sperm parameters quality and reproductive effects of methanolic extract of Alchornea cordifolia leaves on senescent male rats [Pubmed: 31353623]
        Journal of Ovarian Research Baicalin ameliorates polycystic ovary syndrome through AMP-activated protein kinase [Pubmed: 31722718]
        Genesis Sleep deprivation decreases the reproductive capacity by affecting the arrival of morulas in the uterus [Pubmed: 31815354]
        Chemosphere Exposure of adult mice to perfluorobutanesulfonate impacts ovarian functions through hypothyroxinemia leading to down-regulation of Akt-mTOR signaling [Pubmed: 31809938]
        Medicina Effect of DHT-Induced Hyperandrogenism on the Pro-Inflammatory Cytokines in a Rat Model of Polycystic Ovary Morphology [Pubmed: 32120970]
        Chemosphere Prenatal exposure of female mice to per?uorononanoic acid delays pubertal activation of the reproductive endocrine axis through enhanced hepatic FGF21?… [Pubmed: 33131727]
        BIOLOGICAL RESEARCH Novel mechanisms underlying anti-polycystic ovary like syndrome effects of electroacupuncture in rats: suppressing SREBP1 to mitigate insulin resistance?… [Pubmed: 33109277]
        ARCHIVES OF GYNECOLOGY AND OBSTETRICS MiRNAs expression profiling of rat ovaries displaying PCOS with insulin resistance [Pubmed: 32757043]
        Mol Med Electroacupuncture alleviates polycystic ovary syndrome-like symptoms through improving insulin resistance, mitochondrial dysfunction, and endoplasmic?… [Pubmed: 32698821]
        Antioxidants (Basel) Composition of Royal Jelly (RJ) and Its Anti-Androgenic Effect on Reproductive Parameters in a Polycystic Ovarian Syndrome (PCOS) Animal Model [Pubmed: 32517356]
        chemosphere N, N-Dimethylacetamide, an FDA approved excipient, acts post-meiotically to impair spermatogenesis and cause infertility in rats [Pubmed: 32447106]
        Neurotoxicology Chronic exposure to low dose of bisphenol A causes follicular atresia by inhibiting kisspeptin neurons in anteroventral periventricular nucleus in female mice [Pubmed: 32407858]
        BioMed Research International Pathophysiological Changes in Female Rats with Estrous Cycle Disorder Induced by Long-Term Heat Stress [Pubmed: 32685488]
        ACS Applied Bio Materials Evaluation for adverse effects of InP/ZnS quantum dots on the in vitro cultured oocytes of mice []
        Iranian Journal of Basic Medical Sciences Therapeutic effects of organic zinc on reproductive hormones, insulin resistance and mTOR expression as a novel component in a rat model of PCOS []
        Medical Journal of Cairo University Hemin Ameliorated Letrozole-Induced Polycystic Ovary Syndrome: A Forward Step for Better Management []
        biorxiv The effects of human umbilical cord mesenchymal stem cell transplantation on female fertility preservation in mice []
        Drug-Herb Interaction Between Selective Estrogen Receptor Modulators and Estrogenic Herbal Medicine Er-Xian Decoction in Bone in Vivo and in Vitro []
        JOURNAL OF ETHNOPHARMACOLOGY Zihuai recipe alleviates cyclophosphamide-induced diminished ovarian reserve via suppressing PI3K/AKT-mediated apoptosis [33422655]
        advanced biomedical research The Effects of Zinc Methionine on Reproductive and Thyroid Hormones in Rats with Polycystic Ovarian Syndrome [33457340]
        Endocrinology Diabetes Type 1 Negatively Influences Leydig Cell Function in Rats, Which is Partially Reversible By Insulin Treatment [33507237]
        JOURNAL OF ETHNOPHARMACOLOGY BaZiBuShen alleviates altered testicular morphology and spermatogenesis and modulates Sirt6/P53 and Sirt6/NF-|êB pathways in aging mice induced by D-galactose [33508368]
        Journal of Ethnopharmacology Water extract of Er-xian decoction selectively exerts estrogenic activities and interacts with SERMs in estrogen-sensitive tissues [33823166]
        Toxicol Appl Pharmacol Exposure to the bromodomain inhibitor N-methyl pyrrolidone blocks spermatogenesis in a hormonal and non-hormonal fashion [33965371]
        researchsquare Lipoamino Acid-Modified GnRH Analogs with Receptor-Mediated Antiproliferative Activity in Prostate and Ovarian Cancer Cells []
        Scientific reports Regulation of Uterine Function During Estrous Cycle, Anestrus Phase and Pregnancy by Steroids in Red Deer (Cervus elaphus L.) []
        Chronic Exposure to Perfluorohexane Sulfonate Leads to a Reproduction Deficit by Suppressing Hypothalamic Kisspeptin Expression in Mice []
        Comparison of ovarian striation and ovarian fragmentation in a rat model of ovarian insufficiency []
        JOURNAL OF INHERITED METABOLIC DISEASE Human and animal fertility studies in cystinosis reveal signs for obstructive azoospermia, a disruptive blood‐testis barrier and a subtherapeutic effect of cysteamine in?… [34494673]
        Bioengineered MiR-1224-5p attenuates polycystic ovary syndrome through inhibiting NOD-like receptor protein 3 inflammasome activation via targeting Forkhead box O 1 [34637688]
        Free Radical Biology and Medicine Cadmium attenuates testosterone synthesis by promoting ferroptosis and blocking autophagosome-lysosome fusion [34610361]
        Prostate Endothelial dysfunction after androgen deprivation therapy and the possible underlying mechanisms [34570375]
        Microbiome Polyamine metabolism links gut microbiota and testicular dysfunction [34758869]
        J Virol Zika virus infection in the ovary induces continuously elevated progesterone level and compromises conception in interferon α/β receptor-deficient mice [34730391]
        Stem Cells Int Activated Human Umbilical Cord Blood Platelet-Rich Plasma Enhances the Beneficial Effects of Human Umbilical Cord Mesenchymal Stem Cells in?… [34733331]
        Cell Death Dis METTL3-mediated mRNA N6-methyladenosine is required for oocyte and follicle development in mice [34689175]
        Theriogenology Superior effect of broccoli methanolic extract on control of oxidative damage of sperm cryopreservation and reproductive performance in rats: A comparison with?… [Pubmed:35063921]
        Nutrients Yogurt Enriched with Inulin Ameliorated Reproductive Functions and Regulated Gut Microbiota in Dehydroepiandrosterone-Induced Polycystic Ovary Syndrome Mice [Pubmed:35057459]
        Nat Commun TrkB agonist antibody ameliorates fertility deficits in aged and cyclophosphamide-induced premature ovarian failure model mice [Pubmed:35177657]
        Sci Total Environ Exposure to multiple pyrethroid insecticides affects ovarian follicular development via modifying microRNA expression [Pubmed:35276145]
        Ecotoxicology and Environmental Safety Constitutional delay of growth and puberty in female mice is induced by circadian rhythm disruption in utero [Pubmed:35679725]
        Stem Cell Research & Therapy G-CSF-mobilized Peripheral Blood Mononuclear Cells Combined with Platelet-rich Plasma Restored the Ovarian Function of aged rats via angiogenesis and?… []
        Research Square Drug-free in vitro activation combined 3D-bioprinted adipose-derived stem cells restore ovarian function of premature ovarian insufficiency []
        BMC Veterinary Research A promising animal model for Polycystic Ovary Syndrome: Borderline Hypertensive Rats []
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        亚洲精品国产情侣AV在线,日韩精品一区二区三区色欲AV,免费看高清黄A级毛片