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      1. 生長激素(GH)檢測試劑盒(酶聯免疫吸附試驗法)

        ELISA Kit for Growth Hormone (GH)

        GH1; GH-N; GHN; hGH-N; Somatotropin; Hygetropin; Jintropin; Kigtropin; Pituitary Growth Hormone; Growth Hormone, Normal

        • 生長激素(GH)檢測試劑盒(酶聯免疫吸附試驗法) 產品包裝(模擬)
        • 生長激素(GH)檢測試劑盒(酶聯免疫吸附試驗法) 產品包裝(模擬)
        • 生長激素(GH)檢測試劑盒(酶聯免疫吸附試驗法) 實驗結果圖
        • SEA044Hu.jpg 標準曲線圖
        • Certificate 通過ISO 9001、ISO 13485質量體系認證

        特異性

        本試劑盒用于檢測生長激素(GH),經檢測與其它相似物質無明顯交叉反應。
        由于受到技術及樣本來源的限制,不可能完成對所有相關或相似物質交叉反應檢測,因此本試劑盒有可能與未經檢測的其它物質有交叉反應。

        回收率

        分別于定值血清及血漿樣本中加入一定量的生長激素(GH)(加標樣品),重復測定并計算其均值,回收率為測定值與理論值的比率。

        樣本 回收率范圍(%) 平均回收率(%)
        serum(n=5) 85-101 90
        EDTA plasma(n=5) 94-103 98
        heparin plasma(n=5) 90-104 94

        精密度

        精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
        批內差:取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續測定20 次,分別計算不同濃度樣本的平均值及SD值。
        批間差:選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定,每個樣本使用同一試劑盒重復測定8次,分別計算不同濃度樣本的平均值及SD值。
        批內差: CV<10%
        批間差: CV<12%

        線性

        在定值血清及血漿樣本內加入適量的生長激素(GH),并倍比稀釋成1:2,1:4,1:8,1:16的待測樣本,線性范圍即為稀釋后樣本中生長激素(GH)含量的測定值與理論值的比率。

        樣本 1:2 1:4 1:8 1:16
        serum(n=5) 79-102% 79-90% 89-102% 92-105%
        EDTA plasma(n=5) 89-96% 78-93% 87-96% 80-90%
        heparin plasma(n=5) 87-96% 84-94% 87-101% 94-101%

        穩定性

        經測定,試劑盒在有效期內按推薦溫度保存,其活性降低率小于5%。
        為減小外部因素對試劑盒破壞前后檢測值的影響,實驗室的環境條件需盡量保持一致,尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

        實驗流程

        1. 實驗前標準品、試劑及樣本的準備;
        2. 加樣(標準品及樣本)100µL,37°C孵育1小時;
        3. 吸棄,加檢測溶液A100µL,37°C孵育1小時;
        4. 洗板3次;
        5. 加檢測溶液B100µL,37°C孵育30分鐘;
        6. 洗板5次;
        7. 加TMB底物90µL,37°C孵育10-20分鐘;
        8. 加終止液50µL,立即450nm讀數。

        實驗原理

        將生長激素(GH)抗體包被于96孔微孔板中,制成固相載體,向微孔中分別加入標準品或標本,其中的生長激素(GH)與連接于固相載體上的抗體結合,然后加入生物素化的生長激素(GH)抗體,將未結合的生物素化抗體洗凈后,加入HRP標記的親和素,再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉化成藍色,并在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中的生長激素(GH)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值),計算樣品濃度。

        相關產品

        編號 適用物種:Homo sapiens (Human,人) 應用(僅供研究使用,不用于臨床診斷!)
        APA044Hu01 生長激素(GH)活性蛋白 Cell?culture;?Activity?Assays.
        EPA044Hu61 生長激素(GH)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
        RPA044Hu01 生長激素(GH)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
        PAA044Hu06 生長激素(GH)多克隆抗體 WB; IHC; ICC; IP.
        PAA044Hu01 生長激素(GH)多克隆抗體 WB; IHC; ICC; IP.
        MAA044Hu22 生長激素(GH)單克隆抗體 WB; IHC; ICC; IP.
        MAA044Hu26 生長激素(GH)單克隆抗體 WB; IHC; ICC; IP.
        LAA044Hu72 生長激素(GH)單克隆抗體(生物素標記) WB; IHC; ICC.
        SEA044Hu 生長激素(GH)檢測試劑盒(酶聯免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
        CEA044Hu 生長激素(GH)檢測試劑盒(酶聯免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
        AEA044Hu 抗生長激素抗體(Anti-GH)檢測試劑盒(酶聯免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antibody Detection.
        SCA044Hu 生長激素(GH)檢測試劑盒(化學發光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
        LMA044Hu 生長激素(GH)單因子檢測試劑盒(流式熒光發光法) Magnetic Luminex Assay for Antigen Detection.
        PSA044Hu01 生長激素(GH)抗體對 ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
        KSA044Hu01 生長激素(GH)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) Main materials for "Do It (ELISA Kit) Yourself".

        參考文獻

        雜志 參考文獻
        Neuroscience Letters The effect of peripheral administration of growth hormone on AD-like cognitive deficiency in NBM-lesioned rats [PubMed: 19765635]
        Cellular Reprogramming Changes in the Somatotrophic Axis in Genetically Identical Dogs [PubMed: 21740269]
        Clinical Cancer Research The efficacy of IGF-I receptor monoclonal antibody against human gastrointestinal carcinomas is independent of k-ras mutation status [PubMed: 21642381]
        General and Comparative Endocrinology Growth hormone expression and neuroprotective activity in a quail neural retina cell line [ScienceDirect: S0016648009002457]
        Nutrition and Metabolism Periodic 48 h feed withdrawal improves glucose tolerance in growing pigs by enhancing adipogenesis and lipogenesis [Nutrition&Metabolism: source]
        Veterinary Recprd Case Report Treatment of cervical metaphyseal fractures due to delayed ossification in an adult dog with hypopituitarism [Bmj: Source]
        Journal of the American Animal Hospital Association GH Deficiency in a Dalmatian Puppy with Megaesophagus [Pubmed: 24051259]
        Biotechnology and Bioprocess Engineering Functional expression of bovine growth hormone gene in Pleurotus eryngii [Springer: Source]
        Buletin Veteriner Udayana Profil Growth Hormone Sapi Bali Betina Pada Tiga Tipe Lahan Pemeliharaan Di Provinsi Bali [Unud:Source]
        Veterinary World Effect of prill fat supplementation on hormones, milk production and energy metabolites during mid lactation in crossbred cows [Veterinaryworld:Source]
        Int J Clin Exp Med Effects of sericin on the testicular growth hormone/insulin-like growth factor-1 axis in a rat model of type 2 diabetes [PubMed: 26379831]
        Acta Histochemica Melatonin modulates monochromatic light-induced GHRH expression in the hypothalamus and GH secretion in chicks [Pubmed:26948666]
        ANNALS OF ANIMAL SCIENCE Comparison of the effect of a standard inclusion level of inorganic zinc to organic form at lowered level on bone development in growing male Ross broiler chickens [journalprintahead:87]
        Annals of Animal Science Comparison of the Effect of a Standard Inclusion Level of Inorganic Zinc to Organic Form at Lowered Level on Bone Development in Growing Male Ross Broiler Chickens [view:87]
        Journal?of?Dairy?Science Effect of thermal stress on physiological, hormonal and haematological parameters in Tharparkar and Karan Fries calves [publication:306322503]
        Jurnal Riset Akuakultur TRANSMISI GEN PhGH DAN PERFORMA PERTUMBUHAN IKAN LELE AFRIKA (Clarias gariepinus) TRANSGENIK GENERASI KETIGA [1329]
        Trop Anim Health Prod Assessment of feeding varying levels of Metabolizable energy and protein on performance of transition Murrah buffaloes [pubmed:28785907]
        Biological Trace Element Research Effect of Dietary Phytase Supplementation on Bone and Hyaline Cartilage Development of Broilers Fed with Organically Complexed Copper in a Cu-Deficient Diet [article:10.1007]
        Revista Brasileira de Ciência Avícola Effect of Zinc Level and Source (Zinc Oxide Vs. Zinc Glycine) on Bone Mechanical and Geometric Parameters, and Histomorphology in Male Ross 308 Broiler Chicken [10.159:1806-9061-2016-0285]
        畜牧獸醫學報 單色綠光對雞胚褪黑激素合成的影響 [DOI: 10.11843/j.issn.0366-6964.2017.05.019]
        Obesity Surgery Effects of Bariatric Surgery on Change of Brown Adipocyte Tissue and Energy Metabolism in Obese Mice [pubmed:28853013]
        JURNAL VETERINER Kadar Hormon Pertumbuhan Sapi Bali Lebih Rendah di Nusa Penida Daripada Daerah Bali Lainnya(LEVELS OF GROWTH HORMONE BALI CATTLE IN NUSA PENIDA LOWER THAN OTHER BALI REGIONS) [10.19087/jveteriner.2017.18.2.226]
        Veterinary World. Factors influencing growth hormone levels of Bali cattle in Bali, Nusa Penida, and Sumbawa Islands, Indonesia. [pubmed:29184372]
        Biochemistry, Genetics and Molecular Biology Effects of Dietary Lysine Levels on the Plasma Concentrations of Growth‐Related Hormones in Late‐Stage Finishing Pigs [10.5772/intechopen.68545]
        Cellular Physiology and Biochemistry Decreased Hepatic Lactotransferrin Induces Hepatic Steatosis in Chronic Non-Alcoholic Fatty Liver Disease Model [Pubmed:29975946]
        PLoS One Subsequent somatic axis and bone tissue metabolism responses to a low-zinc diet with or without phytase inclusion in broiler chickens [Pubmed:29373588]
        Pakistan Journal of Zoology Effects of Intrauterine Growth Retardation on Growth, Meat Quality and Muscle Fiber Composition of Pigs. [:]
        Cell?Reports Zika Virus Infection in Hypothalamus Causes Hormone Deficiencies and Leads to Irreversible Growth Delay and Memory Impairment in Mice [Pubmed: 30404008]
        International Journal of Current Microbiology and Applied Sciences Physiological Performance of Crossbred Cattle Calves (Karan Fries) under Different Housing Conditions during Different Seasons []
        Photochemistry and Photobiology Melatonin Receptor Mel1b‐and Mel1c‐mediated Green Light Induced the Secretion of Growth Hormone in Anterior Pituitary of Chicks [Pubmed: 31111492]
        Journal of Animal Science Dietary lysine affects amino acid metabolism and growth performance, which may not involve the GH/IGF-1 axis, in young growing pigs [Pubmed: 31922564]
        SAUDI MEDICAL JOURNAL The evaluation of leukocyte-platelet rich fibrin as an anti-inflammatory autologous biological additive [Pubmed: 31287125]
        Frontiers in Endocrinology RFRP-3, the Mammalian Ortholog of GnIH, Is a Novel Modulator Involved in Food Intake and Glucose Homeostasis [Pubmed: 32328034]
        JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION Isolated growth hormone deficiency in a Chihuahua with a GH1 mutation [Pubmed: 32646299]
        Scientific Reports Controlled elevated temperatures during early-mid gestation cause placental insufficiency and implications for fetal growth in pregnant pigs [33244103]
        Animal Effect of alfalfa hay and starter feed supplementation on caecal microbiota and fermentation, growth, and health of yak calves [33610512]
        JOURNAL OF CELLULAR AND MOLECULAR MEDICINE GHR is involved in gastric cell growth and apoptosis via PI3K/AKT signalling [33492754]
        animals Effects of Concentrate and Bypass Fat Supplementations on Growth Performance, Blood Profile, and Rearing Cost of Feedlot Buffaloes [34359233]
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        亚洲精品国产情侣AV在线,日韩精品一区二区三区色欲AV,免费看高清黄A级毛片