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      1. 肝細胞生長因子(HGF)檢測試劑盒(酶聯免疫吸附試驗法)

        ELISA Kit for Hepatocyte Growth Factor (HGF)

        HGF; F-TCF; HGFB; HPTA; SF; Scatter Factor; Hepapoietin A; Fibroblast-Derived Tumor Cytotoxic Factor; Lung Fibroblast-Derived Mitogen

        • 肝細胞生長因子(HGF)檢測試劑盒(酶聯免疫吸附試驗法) 產品包裝(模擬)
        • 肝細胞生長因子(HGF)檢測試劑盒(酶聯免疫吸附試驗法) 產品包裝(模擬)
        • 肝細胞生長因子(HGF)檢測試劑盒(酶聯免疫吸附試驗法) 實驗結果圖
        • SEA047Hu.jpg 標準曲線圖
        • Certificate 通過ISO 9001、ISO 13485質量體系認證

        特異性

        本試劑盒用于檢測肝細胞生長因子(HGF),經檢測與其它相似物質無明顯交叉反應。
        由于受到技術及樣本來源的限制,不可能完成對所有相關或相似物質交叉反應檢測,因此本試劑盒有可能與未經檢測的其它物質有交叉反應。

        回收率

        分別于定值血清及血漿樣本中加入一定量的肝細胞生長因子(HGF)(加標樣品),重復測定并計算其均值,回收率為測定值與理論值的比率。

        樣本 回收率范圍(%) 平均回收率(%)
        serum(n=5) 87-94 91
        EDTA plasma(n=5) 94-101 97
        heparin plasma(n=5) 93-102 97

        精密度

        精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
        批內差:取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續測定20 次,分別計算不同濃度樣本的平均值及SD值。
        批間差:選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定,每個樣本使用同一試劑盒重復測定8次,分別計算不同濃度樣本的平均值及SD值。
        批內差: CV<10%
        批間差: CV<12%

        線性

        在定值血清及血漿樣本內加入適量的肝細胞生長因子(HGF),并倍比稀釋成1:2,1:4,1:8,1:16的待測樣本,線性范圍即為稀釋后樣本中肝細胞生長因子(HGF)含量的測定值與理論值的比率。

        樣本 1:2 1:4 1:8 1:16
        serum(n=5) 91-101% 80-94% 85-103% 96-103%
        EDTA plasma(n=5) 78-97% 94-103% 80-90% 83-94%
        heparin plasma(n=5) 90-103% 96-103% 87-103% 91-105%

        穩定性

        經測定,試劑盒在有效期內按推薦溫度保存,其活性降低率小于5%。
        為減小外部因素對試劑盒破壞前后檢測值的影響,實驗室的環境條件需盡量保持一致,尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

        實驗流程

        1. 實驗前標準品、試劑及樣本的準備;
        2. 加樣(標準品及樣本)100µL,37°C孵育1小時;
        3. 吸棄,加檢測溶液A100µL,37°C孵育1小時;
        4. 洗板3次;
        5. 加檢測溶液B100µL,37°C孵育30分鐘;
        6. 洗板5次;
        7. 加TMB底物90µL,37°C孵育10-20分鐘;
        8. 加終止液50µL,立即450nm讀數。

        實驗原理

        將肝細胞生長因子(HGF)抗體包被于96孔微孔板中,制成固相載體,向微孔中分別加入標準品或標本,其中的肝細胞生長因子(HGF)與連接于固相載體上的抗體結合,然后加入生物素化的肝細胞生長因子(HGF)抗體,將未結合的生物素化抗體洗凈后,加入HRP標記的親和素,再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉化成藍色,并在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中的肝細胞生長因子(HGF)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值),計算樣品濃度。

        相關產品

        編號 適用物種:Homo sapiens (Human,人) 應用(僅供研究使用,不用于臨床診斷!)
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        PAA047Hu01 肝細胞生長因子(HGF)多克隆抗體 WB; IHC; ICC; IP.
        PAA047Hu02 肝細胞生長因子(HGF)多克隆抗體 WB; IHC; ICC; IP.
        PAA047Hu03 肝細胞生長因子(HGF)多克隆抗體 WB; IHC; ICC; IP.
        LAA047Hu71 肝細胞生長因子(HGF)多克隆抗體(生物素標記) WB; IHC; ICC.
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        LAA047Hu81 肝細胞生長因子(HGF)多克隆抗體(異硫氰酸熒光素標記) WB; IHC; ICC; IF.
        MAA047Hu22 肝細胞生長因子(HGF)單克隆抗體 WB; IHC; ICC; IP.
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        KSA047Hu01 肝細胞生長因子(HGF)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) Main materials for "Do It (ELISA Kit) Yourself".

        參考文獻

        雜志 參考文獻
        Gene Therapy and Molecular Biology Sphingosine kinase-1 protects transplanted mesenchymal stem cells and improves the performance of the infarcted heart [LWW: 01000]
        Journal of Translational Medicine Low temperature of radiofrequency ablation at the target sites can facilitate rapid progression of residual hepatic VX2 carcinoma [BioMed: 14795876]
        Digestive Diseases and Sciences Gene Transfer of c-met Confers Protection Against d-Galactosamine/Lipopolysaccharide-Induced Acute Liver Failure [SpringerLink: k22823l138x2344x]
        Biomarker insights Correlation Between Hepatocyte Growth Factor (HGF) and Gamma-Aminobutyric Acid (GABA) Plasma Levels in Autistic Children [PubMed: PMC3694825]
        Biomarker insights Decreased Hepatocyte Growth Factor (HGF) and Gamma Aminobutyric Acid (GABA) in Individuals with Obsessive-Compulsive Disorder (OCD) [PubMed: PMC3762604]
        BMC cell biology BMSCs reduce rat granulosa cell apoptosis induced by cisplatin and perimenopause [PubMed: PMC3640998]
        Cell Journal (Yakhteh) Effect of Mesenchymal Stem Cells on Doxorubicin-Induced Fibrosis [PubMed: PMC3584430]
        Gastroenterol Res Pract Proinflammatory Cytokines (IL-1α, IL-6) and Hepatocyte Growth Factor in Patients with Alcoholic Liver Cirrhosis [PubMed: 26448742]
        Tumor Biology Hypoxia-induced secretion of IL-11 from adipose-derived mesenchymal stem cell promotes growth and cancer stem cell properties of Burkitt lymphoma [PubMed: 26695151]
        Cytotherapy Cell type–dependent variation in paracrine potency determines therapeutic efficacy against neonatal hyperoxic lung injury [PubMed: 25863963]
        Biomedicine & Pharmacotherapy Silymarin-loaded Eudragit? RS100 nanoparticles improved the ability of silymarin to resolve hepatic fibrosis in bile duct ligated rats [Pubmed:27261582]
        Can J Physiol Pharmacol.? Piceatannol increases the expression of hepatocyte growth factor and IL-10 thereby protecting hepatocytes in thioacetamide-induced liver fibrosis [Pubmed:27186801]
        Genet Mol Res Changes in growth factor levels in the cerebrospinal fluid of autism patients after transplantation of human umbilical cord blood mononuclear cells and umbilical cord-derived mesenchymal stem cells [Pubmed:27323064]
        Changes in HGF levels after a progressive test before and after Tabata HIIT program [profile:Pawel_Wolanski3]
        Stem Cell Research & Therapy Effects of low-intensity pulsed ultrasound (LIPUS)-pretreated human amnion-derived mesenchymal stem cell (hAD-MSC) transplantation on primary ovarian insufficiency in rats [10.1186/s13287-017-0739-3]
        Oncology?Letters Carcinoma associated fibroblasts derived from oral squamous cell carcinoma promote lymphangiogenesis via c?Met/PI3K/AKT in vitro [10.3892/ol.2017.7301]
        Stem Cell Research & Therapy Overexpression of c-Met in bone marrow mesenchymal stem cells improves their effectiveness in homing and repair of acute liver failure. [pubmed:28679425]
        Nature?Communications Pulmonary pericytes regulate lung morphogenesis [Pubmed:29934496]
        Experimental And Molecular?Medicine Interleukin-1 receptor antagonist-mediated neuroprotection by umbilical cord-derived mesenchymal stromal cells following transplantation into a rodent stroke?… [Pubmed:29650950]
        Oncology?Letters Carcinoma associated fibroblasts derived from oral squamous cell carcinoma promote lymphangiogenesis via c-Met/PI3K/AKT in vitro [Pubmed:29375714]
        Bulletin?of Experimental?Biology?and?Medicine Dynamics of Expression of Cytokine Genes and Macrophage Content in the Lungs and Kidneys after Subtotal Hepatectomy in Rats [Pubmed:29796817]
        Cell Biology International Inherent control of hepatocyte proliferationInherent control of hepatocyte proliferation after subtotal liver resection [Pubmed: 31297922]
        Journal of stem cells & regenerative medicine What is the Best Degree of Hyaluronic Acid Crosslinking in Increasing Growth Factors Level of Platelet-Rich Fibrin Lysate? [Pubmed: 31239605]
        Open Journal of Veterinary Medicine Efficacy of autologous bone marrow mononuclear cell transplantation in dogs with chronic spinal cord injury [Pubmed: 32821665]
        Stem Cell Reviews and Reports Prospects on the Potential In Vitro Regenerative Features of Mechanically Treated-Adipose Tissue for Osteoarthritis Care [33469783]
        Molecular Biology Reports Umbilical cord-derived mesenchymal stem cell conditioned medium reverses neuronal oxidative injury by inhibition of TRPM2 activation and the JNK signaling?… [Pubmed:35585377]
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        亚洲精品国产情侣AV在线,日韩精品一区二区三区色欲AV,免费看高清黄A级毛片