亚洲精品国产情侣AV在线,日韩精品一区二区三区色欲AV,免费看高清黄A级毛片

  • <object id="jwsct"><legend id="jwsct"></legend></object>
    <object id="jwsct"></object>
  • <s id="jwsct"></s>
      1. 細胞間粘附分子1(ICAM1)檢測試劑盒(酶聯免疫吸附試驗法)

        ELISA Kit for Intercellular Adhesion Molecule 1 (ICAM1)

        CD54; BB2; P3.58; Human Rhinovirus Receptor; Major group rhinovirus receptor

        • 細胞間粘附分子1(ICAM1)檢測試劑盒(酶聯免疫吸附試驗法) 產品包裝(模擬)
        • 細胞間粘附分子1(ICAM1)檢測試劑盒(酶聯免疫吸附試驗法) 產品包裝(模擬)
        • 細胞間粘附分子1(ICAM1)檢測試劑盒(酶聯免疫吸附試驗法) 實驗結果圖
        • SEA548Hu.jpg 標準曲線圖
        • Certificate 通過ISO 9001、ISO 13485質量體系認證

        特異性

        本試劑盒用于檢測細胞間粘附分子1(ICAM1),經檢測與其它相似物質無明顯交叉反應。
        由于受到技術及樣本來源的限制,不可能完成對所有相關或相似物質交叉反應檢測,因此本試劑盒有可能與未經檢測的其它物質有交叉反應。

        回收率

        分別于定值血清及血漿樣本中加入一定量的細胞間粘附分子1(ICAM1)(加標樣品),重復測定并計算其均值,回收率為測定值與理論值的比率。

        樣本 回收率范圍(%) 平均回收率(%)
        serum(n=5) 90-104 96
        EDTA plasma(n=5) 79-91 78
        heparin plasma(n=5) 78-101 87

        精密度

        精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
        批內差:取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續測定20 次,分別計算不同濃度樣本的平均值及SD值。
        批間差:選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定,每個樣本使用同一試劑盒重復測定8次,分別計算不同濃度樣本的平均值及SD值。
        批內差: CV<10%
        批間差: CV<12%

        線性

        在定值血清及血漿樣本內加入適量的細胞間粘附分子1(ICAM1),并倍比稀釋成1:2,1:4,1:8,1:16的待測樣本,線性范圍即為稀釋后樣本中細胞間粘附分子1(ICAM1)含量的測定值與理論值的比率。

        樣本 1:2 1:4 1:8 1:16
        serum(n=5) 94-105% 98-105% 94-102% 84-95%
        EDTA plasma(n=5) 85-102% 89-96% 97-104% 91-101%
        heparin plasma(n=5) 83-101% 86-103% 94-102% 79-104%

        穩定性

        經測定,試劑盒在有效期內按推薦溫度保存,其活性降低率小于5%。
        為減小外部因素對試劑盒破壞前后檢測值的影響,實驗室的環境條件需盡量保持一致,尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

        實驗流程

        1. 實驗前標準品、試劑及樣本的準備;
        2. 加樣(標準品及樣本)100µL,37°C孵育1小時;
        3. 吸棄,加檢測溶液A100µL,37°C孵育1小時;
        4. 洗板3次;
        5. 加檢測溶液B100µL,37°C孵育30分鐘;
        6. 洗板5次;
        7. 加TMB底物90µL,37°C孵育10-20分鐘;
        8. 加終止液50µL,立即450nm讀數。

        實驗原理

        將細胞間粘附分子1(ICAM1)抗體包被于96孔微孔板中,制成固相載體,向微孔中分別加入標準品或標本,其中的細胞間粘附分子1(ICAM1)與連接于固相載體上的抗體結合,然后加入生物素化的細胞間粘附分子1(ICAM1)抗體,將未結合的生物素化抗體洗凈后,加入HRP標記的親和素,再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉化成藍色,并在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中的細胞間粘附分子1(ICAM1)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值),計算樣品濃度。

        相關產品

        編號 適用物種:Homo sapiens (Human,人) 應用(僅供研究使用,不用于臨床診斷!)
        RPA548Hu02 細胞間粘附分子1(ICAM1)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
        RPA548Hu01 細胞間粘附分子1(ICAM1)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
        PAA548Hu01 細胞間粘附分子1(ICAM1)多克隆抗體 WB; IHC; ICC; IP.
        PAA548Hu02 細胞間粘附分子1(ICAM1)多克隆抗體 WB; IHC; ICC; IP.
        LAA548Hu71 細胞間粘附分子1(ICAM1)多克隆抗體(生物素標記) WB; IHC; ICC.
        LAA548Hu81 細胞間粘附分子1(ICAM1)多克隆抗體(異硫氰酸熒光素標記) WB; IHC; ICC; IF.
        MAA548Hu22 細胞間粘附分子1(ICAM1)單克隆抗體 WB; IHC; ICC; IP.
        MAA548Hu21 細胞間粘附分子1(ICAM1)單克隆抗體 WB; IHC; ICC; IP.
        SEA548Hu 細胞間粘附分子1(ICAM1)檢測試劑盒(酶聯免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
        SCA548Hu 細胞間粘附分子1(ICAM1)檢測試劑盒(化學發光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
        LMA548Hu 細胞間粘附分子1(ICAM1)單因子檢測試劑盒(流式熒光發光法) Magnetic Luminex Assay for Antigen Detection.

        參考文獻

        雜志 參考文獻
        Amran et al. Lipids in Health and Disease Changes in the vascular cell adhesion molecule-1, intercellular adhesion molecule-1 and c-reactive protein following administration of aqueous extract of piper sarmentosum on experimental. [BioMed: 1476511X]
        Evidence-based Complementary and Alternative Medicine Preventive Effects of a Natural Anti-Inflammatory Agent, Astragaloside IV, on Ischemic Acute Kidney Injury in Rats [PubMed: PMC3703719]
        British Journal of Medical and Health Sciences EFFECTS OF ADRIAMYCIN, CISPLATIN, AND 5-FLUOROURACIL ON THE TESTES OF ALBINO RATS [Researchgate: Source]
        Cytokine Astragaloside IV ameliorates renal injury in streptozotocin-induced diabetic rats through inhibiting NF-κB-mediated inflammatory genes expression. [Pubmed: 23434274]
        BioMed research international Endothelial gene expression and molecular changes in response to radiosurgery in in vitro and in vivo models of cerebral arteriovenous malformations [Pubmed: 24199192]
        Journal of Veterinary Internal Medicine Markers of coagulation activation, endothelial stimulation, and inflammation in dogs with babesiosis [Pubmed: 23875771]
        Arch Dis Child PO-0699 Estimation Of Adhesion Molecules Intrauterine Growth Retardation (iugr) Newborns [Bmj:Source]
        Arch Dis Child? PO-0701 Causes Of Fetal Growth Restriction And Early Neonatal Outcome [Bmj:Source]
        Arch Dis Child PO-0698 Neonatal Resuscitation Practices In The Delivery Room In India: An Audit Using Videography [Bmj:Source]
        J Thorac Dis.? Anti-endothelial cell antibodies in connective tissue diseases associated with pulmonary arterial hypertension [Pubmed:Pmc4015009]
        Journal of Immunotoxicology Acacia ferruginea inhibits cyclophosphamide-induced immunosuppression and urotoxicity by modulating cytokines in mice [Pubmed:24873678]
        Free Radical Biology and Medicine Involvement of nitric oxide with activation of Toll-like receptor 4 signaling in mice with dextran sodium sulfate-induced colitis [Pubmed:24992835]
        Int J Neurosci. The effect of therapeutic hypothermia after cardiopulmonary resuscitation on ICAM-1 and NSE levels in sudden cardiac arrest rabbits [Pubmed:25111247]
        Naunyn-Schmiedeberg&#039;s Archives of Pharmacology Insights into the antiatherogenic molecular mechanisms of andrographolide against?Porphyromonas gingivalis-induced atherosclerosis in rabbits [Pubmed:25172523]
        Nutr Res. Curcumin attenuates adhesion molecules and matrix metalloproteinase expression in hypercholesterolemic rabbits [Pubmed:25282128]
        Experimental &amp; Molecular Medicine Elevated levels of serum sICAM-1 in asphyxiated low birth weight newborns [Pubmed:25358349]
        Neural Regen Res.? Inflammatory response and neuronal necrosis in rats with cerebral ischemia [Pubmed:25422636]
        Chemical Research in Toxicology Sub-chronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwall Carbon Nanotubes by Intratracheal Instillation [Pubmed:25580880]
        CNS Neuroscience &amp; Therapeutics Peroxisome Proliferator-activated Receptor-γ Agonist Pioglitazone Ameliorates White Matter Lesion and Cognitive Impairment in Hypertensive Rats [Pubmed:25611692]
        Alexandria Journal of Medicine Effect of consumption of fresh and heated virgin coconut oil on the blood pressure and inflammatory biomarkers: An experimental study in Sprague Dawley rats [Sciencedirect:S2090506814000268]
        Canadian Journal of Physiology and Pharmacology Effect of magnesium sulfate and thyroxine on inflammatory markers in a rat model of hypothyroidism [Doi: Abs]
        J Cell Biochem Tumor Necrosis Factor-Alpha Up-Regulates ICAM-1 Expression and Release in Intestinal Myofibroblasts by Redox-Dependent and -Independent Mechanisms [PubMed: 26177712]
        Neurotoxicology Allopurinol reduces severity of delayed neurologic sequelae in experimental carbon monoxide toxicity in rats [PubMed: 25845300]
        Alexandria Journal of Medicine Effect of consumption of fresh and heated virgin coconut oil on the blood pressure and inflammatory biomarkers: An experimental study in [Science: Article]
        Chemical Research in Toxicology Subchronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwalled Carbon Nanotubes by Intratracheal Instillation [PubMed: 25580880]
        Veterinary Parasitology Expression of adhesion molecules, chemokines and matrix metallo- proteinases (MMPs) in viable and degenerating stage of Taenia solium metacestode in swine neurocysticercosis [PubMed: 26412140]
        Experimental Eye Research The effect of triamcinolone acetonide or bevacizumab on the levels of proinflammatory cytokines after retinal laser photocoaCavia (Guinea pig )lation in pigmented rabbits [Pubmed:27296072]
        Chinese Chemical Letters Screening the anti-gout traditional herbs from TCM using an in vitro method [science:S1001841716301450]
        Am J Physiol Lung Cell Mol Physiol Systematic phenotyping and correlation of biomarkers with lung function and histology in lung fibrosis [Pubmed:26993522]
        Mol Neurobiol A Therapeutic Insight of Niacin and Coenzyme Q10 Against Diabetic Encephalopathy in Rats [Pubmed:26867655]
        Comparative Clinical Pathology Identification of some plasma biomarkers associated with early weaning stress in crossbred piglets [article:10.1007]
        Journal of?Cellular?Biochemistry Tumor Necrosis Factor‐Alpha Up‐Regulates ICAM‐1 Expression and Release in Intestinal Myofibroblasts by Redox‐Dependent and‐Independent Mechanisms [pubmed:26177712]
        BMC Vet Res. Blood markers of fibrinolysis and endothelial activation in canine babesiosis. [pubmed:28363279]
        Biomedicine & pharmacotherapy= Biomedecine & pharmacotherapie Protective effects of N-acetylcystein and atorvastatin against renal and hepatic injury in a rat model of intestinal ischemia-reperfusion [pubmed:28273631]
        Pakistan journal of pharmaceutical sciences Changes in blood pressure, vascular reactivity and inflammatory biomarkers following consumption of heated corn oil. [pubmed:29084680]
        Experimental Parasitology Adhesion molecules, chemokines and matrix metallo-proteinases response after albendazole and albendazole plus steroid therapy in swine neurocysticercosis [pubmed:28821422]
        Molecular?Medicine?Reports Age?related differences in serum MFG?E8, TGF?β1 and correlation to the severity of atherosclerosis determined by ultrasound. [pubmed:29152651]
        Journal of Microbiology, Immunology and Infection Identification of a panel of serum protein markers in early stage of sepsis and its validation in a cohort of patients [pubmed:28655573]
        Journal of Chromatography A Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosis [S0021967317311858]
        International Journal of?Clinical?and Experimental Pathology Protective effect of high mobility group box-1 silence on diabetic retinopathy: an in vivo study [ISSN:1936-2625/IJCEP0055881]
        Oncotarget Global gene expression profile of cerebral ischemia-reperfusion injury in rat MCAO model [pubmed:29088811]
        Microbial Pathogenesis Protective effects of sinomenine against LPS-induced inflammation in piglets. [pubmed:28757275]
        Journal of ophthalmology Panretinal Photocoagulation Using Short-Pulse Laser Induces Less Inflammation and Macular Thickening in Patients with Diabetic Retinopathy [pubmed:28761761]
        The Journal of Biological Chemistry Toll-like receptor 2 deficiency hyperactivates the FoxO1 transcription factor and induces aging-associated cardiac dysfunction in mice [Pubmed:29929978]
        Life?Sciences Lipoxin A4 may attenuate the progression of obesity-related glomerulopathy by inhibiting NF-κB and ERK/p38 MAPK-dependent inflammation [Pubmed:29499280]
        International Journal of Molecular Medicine Aspirin modulates the inflammatory response in a thrombus?stimulated LMVEC model [Pubmed:29568915]
        Clinical?And Experimental Pharmacology And Physiology? Diallyl sulfide alleviates cisplatin‐induced nephrotoxicity in rats via suppressing NF‐κB downstream inflammatory proteins and p53/Puma signalling pathway [Pubmed:29266336]
        Medicine (Baltimore) Rapid detection of urinary soluble intercellular adhesion molecule-1 for determination of lupus nephritis activity [Pubmed:29953010]
        Bioscience Reports N-Acetylcysteine inhalation improves pulmonary function in patients received Liver Transplantation. [Doi: 10.1042/BSR20180858]
        Prostaglandins?&?Other?Lipid?Mediators Detoxification and activating blood circulation decoction reduces restenosis involving the TLR4/NF-κB pathway after balloon injury [Pubmed: 30453089]
        Biochemical and Biophysical Research Communications The anti-inflammatory and anti-oxidative effects of conbercept in treatment of macular edema secondary to retinal vein occlusion [Pubmed: 30558792]
        Nutrition?&?Metabolism L-Carnitine Supplementation Increases Trimethylamine-N-Oxide but not Markers of Atherosclerosis in Healthy Aged Women [Pubmed: 30485835]
        Cytokine Intercellular adhesion molecule-1 (ICAM-1) associates with 24-hour ambulatory blood pressure variability in type 2 diabetes and controls [Pubmed: 30716657]
        European?Journal of Pharmacology The cordycepin derivative IMM-H007 improves endothelial dysfunction by suppressing vascular inflammation and promoting AMPK-dependent eNOS activation in?… [Pubmed: 30826323]
        International journal of molecular sciences Cellular Metabolomics Reveal the Mechanism Underlying the Anti-Atherosclerotic Effects of Aspirin Eugenol Ester on Vascular Endothelial Dysfunction [Pubmed: 31261711]
        Experimental cell research Resveratrol decreases TNFα-induced ICAM-1 expression and release by Sirt-1-independent mechanism in intestinal myofibroblasts [Pubmed: 31233740]
        international journal of molecular sciences Anti-Inflammatory and Anti-Apoptotic Effects of Stybenpropol A on Human Umbilical Vein Endothelial Cells [Pubmed: 31671764]
        Archives of Insect Biochemistry and Physiology Vanadyl sulphate ameliorates biomarkers of endothelial injury and coagulation and thrombosis in a rat model of hyperglycaemia [Pubmed: 31774317]
        BMC Endocrine Disorders The role of the adipocytokines vaspin and visfatin in vascular endothelial function and insulin resistance in obese children [Pubmed: 31771561]
        Scientific Reports Association between Upper-airway Surgery and Ameliorative Risk Markers of endothelial function in obstructive Sleep Apnea [Pubmed: 31882827]
        Journal of Applied Microbiology The effects of dietary sodium butyrate supplementation on the growth performance, carcass traits, and intestinal microbiota of growing‐finishing pigs [Pubmed: 32048746]
        LIFE SCIENCES Intravitreal conbercept improves outcome of proliferative diabetic retinopathy through inhibiting inflammation and oxidative stress [Pubmed: 33227274]
        Metab Brain Dis Ginger and turmeric lipid-solubles attenuate heated oil-induced oxidative stress in the brain via the upregulation of NRF2 and improve cognitive function in rats [Pubmed: 33170419]
        BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE Endothelial epithelial sodium channel involves in high-fat diet-induced atherosclerosis in low-density lipoprotein receptor-deficient mice [Pubmed: 33065235]
        BRITISH JOURNAL OF NUTRITION Ginger and turmeric lipid-solubles attenuate heated oil-induced cardio-hepatic oxidative stress via the upregulation of NRF2 and decrease blood pressure in rats [Pubmed: 33028437]
        MOLECULAR AND CELLULAR BIOCHEMISTRY MicroRNA-217 ameliorates inflammatory damage of endothelial cells induced by oxidized LDL by targeting EGR1 [Pubmed: 32737769]
        OBESITY SURGERY The Protective Effects of Butorphanol on Pulmonary Function of Patients with Obesity Undergoing Laparoscopic Bariatric Surgery: a Double-Blind Randomized … [Pubmed: 32535786]
        Inflammation Tetramethylpyrazine Inhibits Platelet Adhesion and Inflammatory Response in Vascular Endothelial Cells by Inhibiting P38 MAPK and NF-κB Signaling Pathways []
        J Transl Med Multi-omic signatures of atherogenic dyslipidaemia: pre-clinical target identification and validation in humans [33407555]
        Molecules Alpha-Lipoic Acid Plays a Role in Endometriosis: New Evidence on Inflammasome-Mediated Interleukin Production, Cellular Adhesion and Invasion [33430011]
        Antioxidants (Basel) Anti-Atherogenic Effects of Orlistat on Obesity-Induced Vascular Oxidative Stress Rat Model [33562069]
        J Vet Intern Med Association of markers of endothelial activation and dysfunction with occurrence and outcome of pulmonary hemorrhage in dogs with leptospirosis [34076314]
        PLoS One A comparison of the effects of monotherapy with rosuvastatin, atorvastatin or ezetimibe versus combination treatment with rosuvastatin-ezetimibe and?… [34492054]
        Sci. Pharm Dehydroepiandrosterone (DHEA) Improves the Metabolic and Haemostatic Disturbances in Rats with Male Hypogonadism []
        Journal of the South African Veterinary Association Circulating markers of endothelial activation in canine parvoviral enteritis []
        留言咨詢
        亚洲精品国产情侣AV在线,日韩精品一区二区三区色欲AV,免费看高清黄A级毛片