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      1. 內皮型一氧化氮合酶(eNOS)檢測試劑盒(酶聯免疫吸附試驗法)

        ELISA Kit for Endothelial NOS (eNOS)

        NOS3; ECNOS; NOS III; E-NOS; C-NOS; ENOS; CNOS; Constitutive NOS; Nitric Oxide Synthase 3, Endothelial

        • 內皮型一氧化氮合酶(eNOS)檢測試劑盒(酶聯免疫吸附試驗法) 產品包裝(模擬)
        • 內皮型一氧化氮合酶(eNOS)檢測試劑盒(酶聯免疫吸附試驗法) 產品包裝(模擬)
        • 內皮型一氧化氮合酶(eNOS)檢測試劑盒(酶聯免疫吸附試驗法) 實驗結果圖
        • SEA868Hu.jpg 標準曲線圖
        • Certificate 通過ISO 9001、ISO 13485質量體系認證

        特異性

        本試劑盒用于檢測內皮型一氧化氮合酶(eNOS),經檢測與其它相似物質無明顯交叉反應。
        由于受到技術及樣本來源的限制,不可能完成對所有相關或相似物質交叉反應檢測,因此本試劑盒有可能與未經檢測的其它物質有交叉反應。

        回收率

        分別于定值血清及血漿樣本中加入一定量的內皮型一氧化氮合酶(eNOS)(加標樣品),重復測定并計算其均值,回收率為測定值與理論值的比率。

        樣本 回收率范圍(%) 平均回收率(%)
        serum(n=5) 97-105 101
        EDTA plasma(n=5) 95-103 98
        heparin plasma(n=5) 87-105 96

        精密度

        精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
        批內差:取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續測定20 次,分別計算不同濃度樣本的平均值及SD值。
        批間差:選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定,每個樣本使用同一試劑盒重復測定8次,分別計算不同濃度樣本的平均值及SD值。
        批內差: CV<10%
        批間差: CV<12%

        線性

        在定值血清及血漿樣本內加入適量的內皮型一氧化氮合酶(eNOS),并倍比稀釋成1:2,1:4,1:8,1:16的待測樣本,線性范圍即為稀釋后樣本中內皮型一氧化氮合酶(eNOS)含量的測定值與理論值的比率。

        樣本 1:2 1:4 1:8 1:16
        serum(n=5) 94-104% 87-95% 79-98% 96-105%
        EDTA plasma(n=5) 80-91% 87-99% 82-90% 81-99%
        heparin plasma(n=5) 93-101% 81-105% 92-101% 82-98%

        穩定性

        經測定,試劑盒在有效期內按推薦溫度保存,其活性降低率小于5%。
        為減小外部因素對試劑盒破壞前后檢測值的影響,實驗室的環境條件需盡量保持一致,尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

        實驗流程

        1. 實驗前標準品、試劑及樣本的準備;
        2. 加樣(標準品及樣本)100µL,37°C孵育1小時;
        3. 吸棄,加檢測溶液A100µL,37°C孵育1小時;
        4. 洗板3次;
        5. 加檢測溶液B100µL,37°C孵育30分鐘;
        6. 洗板5次;
        7. 加TMB底物90µL,37°C孵育10-20分鐘;
        8. 加終止液50µL,立即450nm讀數。

        實驗原理

        將內皮型一氧化氮合酶(eNOS)抗體包被于96孔微孔板中,制成固相載體,向微孔中分別加入標準品或標本,其中的內皮型一氧化氮合酶(eNOS)與連接于固相載體上的抗體結合,然后加入生物素化的內皮型一氧化氮合酶(eNOS)抗體,將未結合的生物素化抗體洗凈后,加入HRP標記的親和素,再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉化成藍色,并在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中的內皮型一氧化氮合酶(eNOS)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值),計算樣品濃度。

        相關產品

        編號 適用物種:Homo sapiens (Human,人) 應用(僅供研究使用,不用于臨床診斷!)
        RPA868Hu03 內皮型一氧化氮合酶(eNOS)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
        RPA868Hu04 內皮型一氧化氮合酶(eNOS)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
        RPA868Hu01 內皮型一氧化氮合酶(eNOS)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
        PAA868Hu04 內皮型一氧化氮合酶(eNOS)多克隆抗體 WB; IHC; ICC; IP.
        PAA868Hu03 內皮型一氧化氮合酶(eNOS)多克隆抗體 WB; IHC; ICC; IP.
        PAA868Hu01 內皮型一氧化氮合酶(eNOS)多克隆抗體 WB; IHC; ICC; IP.
        LAA868Hu71 內皮型一氧化氮合酶(eNOS)多克隆抗體(生物素標記) WB; IHC; ICC.
        MAA868Hu22 內皮型一氧化氮合酶(eNOS)單克隆抗體 WB; IHC; ICC; IP.
        SEA868Hu 內皮型一氧化氮合酶(eNOS)檢測試劑盒(酶聯免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
        LMA868Hu 內皮型一氧化氮合酶(eNOS)單因子檢測試劑盒(流式熒光發光法) Magnetic Luminex Assay for Antigen Detection.

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