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      1. 骨橋素(OPN)檢測試劑盒(酶聯免疫吸附試驗法)

        ELISA Kit for Osteopontin (OPN)

        SPP1; BNSP; BSPI; ETA1; Secreted Phosphoprotein 1; Bone Sialoprotein I; Early T-Lymphocyte Activation 1; Nephropontin; Urinary stone protein; Uropontin

        • 骨橋素(OPN)檢測試劑盒(酶聯免疫吸附試驗法) 產品包裝(模擬)
        • 骨橋素(OPN)檢測試劑盒(酶聯免疫吸附試驗法) 產品包裝(模擬)
        • 骨橋素(OPN)檢測試劑盒(酶聯免疫吸附試驗法) 實驗結果圖
        • SEA899Hu.jpg 標準曲線圖
        • Certificate 通過ISO 9001、ISO 13485質量體系認證

        特異性

        本試劑盒用于檢測骨橋素(OPN),經檢測與其它相似物質無明顯交叉反應。
        由于受到技術及樣本來源的限制,不可能完成對所有相關或相似物質交叉反應檢測,因此本試劑盒有可能與未經檢測的其它物質有交叉反應。

        回收率

        分別于定值血清及血漿樣本中加入一定量的骨橋素(OPN)(加標樣品),重復測定并計算其均值,回收率為測定值與理論值的比率。

        樣本 回收率范圍(%) 平均回收率(%)
        serum(n=5) 91-99 96
        EDTA plasma(n=5) 96-103 101
        heparin plasma(n=5) 78-104 89

        精密度

        精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
        批內差:取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續測定20 次,分別計算不同濃度樣本的平均值及SD值。
        批間差:選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定,每個樣本使用同一試劑盒重復測定8次,分別計算不同濃度樣本的平均值及SD值。
        批內差: CV<10%
        批間差: CV<12%

        線性

        在定值血清及血漿樣本內加入適量的骨橋素(OPN),并倍比稀釋成1:2,1:4,1:8,1:16的待測樣本,線性范圍即為稀釋后樣本中骨橋素(OPN)含量的測定值與理論值的比率。

        樣本 1:2 1:4 1:8 1:16
        serum(n=5) 87-94% 78-98% 97-104% 79-105%
        EDTA plasma(n=5) 88-97% 93-101% 78-99% 83-101%
        heparin plasma(n=5) 84-97% 78-96% 91-99% 92-104%

        穩定性

        經測定,試劑盒在有效期內按推薦溫度保存,其活性降低率小于5%。
        為減小外部因素對試劑盒破壞前后檢測值的影響,實驗室的環境條件需盡量保持一致,尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

        實驗流程

        1. 實驗前標準品、試劑及樣本的準備;
        2. 加樣(標準品及樣本)100µL,37°C孵育1小時;
        3. 吸棄,加檢測溶液A100µL,37°C孵育1小時;
        4. 洗板3次;
        5. 加檢測溶液B100µL,37°C孵育30分鐘;
        6. 洗板5次;
        7. 加TMB底物90µL,37°C孵育10-20分鐘;
        8. 加終止液50µL,立即450nm讀數。

        實驗原理

        將骨橋素(OPN)抗體包被于96孔微孔板中,制成固相載體,向微孔中分別加入標準品或標本,其中的骨橋素(OPN)與連接于固相載體上的抗體結合,然后加入生物素化的骨橋素(OPN)抗體,將未結合的生物素化抗體洗凈后,加入HRP標記的親和素,再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉化成藍色,并在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中的骨橋素(OPN)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值),計算樣品濃度。

        相關產品

        編號 適用物種:Homo sapiens (Human,人) 應用(僅供研究使用,不用于臨床診斷!)
        EPA899Hu61 骨橋素(OPN)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
        RPA899Hu02 骨橋素(OPN)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
        APA899Hu61 骨橋素(OPN)活性蛋白 Cell?culture;?Activity?Assays.
        PAA899Hu06 骨橋素(OPN)多克隆抗體 WB; IHC; ICC; IP.
        PAA899Hu02 骨橋素(OPN)多克隆抗體 WB; IHC; ICC; IP.
        LAA899Hu71 骨橋素(OPN)多克隆抗體(生物素標記) WB; IHC; ICC.
        MAA899Hu22 骨橋素(OPN)單克隆抗體 WB; IHC; ICC; IP.
        SEA899Hu 骨橋素(OPN)檢測試劑盒(酶聯免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
        AEA899Hu 抗骨橋素抗體(Anti-OPN)檢測試劑盒(酶聯免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antibody Detection.
        SCA899Hu 骨橋素(OPN)檢測試劑盒(化學發光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
        LMA899Hu 骨橋素(OPN)單因子檢測試劑盒(流式熒光發光法) Magnetic Luminex Assay for Antigen Detection.
        KSA899Hu01 骨橋素(OPN)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) Main materials for "Do It (ELISA Kit) Yourself".

        參考文獻

        雜志 參考文獻
        Journal of Biomedical Materials Research Part A Proliferation and osteogenesis of immortalized bone marrow-derived mesenchymal stem cells in porous polylactic glycolic acid scaffolds under perfusion culture [PubMed: 19280635]
        Osteoarthritis and Cartilage Elevated osteopontin level of synovial fluid and articular cartilage is associated with disease severity in knee osteoarthritis patients [PubMed: 19747583]
        The Journal of Rheumatology Thrombin-cleaved osteopontin levels in synovial fluid correlate with disease severity of knee osteoarthritis. [Jrheum: source]
        International Journal of Pharmaceutics Suppression of tumor growth in xenograft model mice by small interfering RNA targeting osteopontin delivery using biocompatible poly(amino ester) [ScienceDirect: S0378517312003742]
        European Cells and matreials The use of non-viral gene vectors for bioactive poly-(D, L-lactide) implant surfaces in bone tissue engineering [Ecmjournal: Source]
        Journal of Equine Veterinary Science Influence of Seminal Plasma Antioxidants and Osteopontin on Fertility of the Arabian Horse [ScienceDirect: Source]
        Basic &amp; Clinical Pharmacology &amp; Toxicology Treatment with Carnitine Enhances Bone Fracture Healing under Osteoporotic and/or Inflammatory Conditions [Wiley:Source]
        Journal of Cellular Biochemistry Tension Force-Induced ATP Promotes Osteogenesis Through P2X7 Receptor in Osteoblasts [Pubmed:24905552]
        Naunyn-Schmiedeberg&#039;s Archives of Pharmacology Treatment with α-lipoic acid enhances the bone healing after femoral fracture model of rats [Pubmed:25038619]
        J Endocrinol Invest.? The interplay among iron metabolism, endothelium and inflammatory cascade in dysmetabolic disorders [Pubmed:25245337]
        FEBS Letters Low-intensity pulsed ultrasound-induced ATP increases bone formation via the P2X7 receptor in osteoblast-like MC3T3-E1 cells [Pubmed:25542352]
        Gene Effects of magnesium degradation products on mesenchymal stem cell fate and osteoblastogenesis [PubMed: 26283150]
        Medicine (Baltimore) An Attempt to Evaluate Selected Aspects of “Bone–Fat Axis” Function in Healthy Individuals and Patients With Pancreatic Cancer [PubMed: 26266370]
        Journal of Biomaterials and Tissue Engineering Osteogenic Differentiation of Canine Adipose Stem Cells Cultured in Alginate-Fibrin-Based Hydrogel [Content: Asp]
        Primasari Osteopontin level correlates negatively with tumor shrinkage in neoadjuvant chemoradiation of locally advanced rectal cancer [Index.Php: Amor]
        Int J Biol Sci Defective Initiation of Liver Regeneration in Osteopontin-Deficient Mice after Partial Hepatectomy due to Insufficient Activation of IL-6/Stat3 Pathway [PubMed: 26327817]
        Journal of Kunming Medical University / Kunming Yike Daxue Xuebao Perioperative Clinical Investigation of Serum VEGF, CA19-9 and OPN Levels in Patients with Colorectal Carcinoma. [Ebscohost]
        Am J Pathol.? Serum Osteopontin as a Novel Biomarker for Muscle Regeneration in Duchenne Muscular Dystrophy. [Pubmed:26963343]
        circulation journal Stenotic Bicuspid and Tricuspid Aortic Valves - Micro-Computed Tomography and Biological Indices of Calcification. [pubmed:28344201]
        Journal of Cardiovascular Electrophysiology Effect of Cardiac Resynchronization Therapy on Myocardial Fibrosis and Relevant Cytokines in a Canine Model With Experimental Heart Failure. [pubmed:28127817]
        American Journal of Translational Research Osteopontin stimulates matrix metalloproteinase expression through the nuclear factor-κB signaling pathway in rat temporomandibular joint and condylar chondrocytes. [pubmed:28337262]
        Diabetes Care Targeted Microbiome Intervention by Microencapsulated Delayed-Release Niacin Beneficially Affects Insulin Sensitivity in Humans [pubmed:29212824]
        Biomedicine & pharmacotherapy Icaritin induces MC3T3-E1 subclone14 cell differentiation through estrogen receptor-mediated ERK1/2 and p38 signaling activation. [pubmed:28742995]
        Journal of?Alzheimers?Disease Urine-Based Biomarkers for Alzheimer's Disease Identified Through Coupling Computational and Experimental Methods [Pubmed:30040720]
        Indian?Journal?of?Nephrology Osteopontin, cardiovascular risk factors and carotid intima-Media thickness in chronic kidney disease [id:233806]
        Carbon In Situ Self-Assembly of Graphene Oxide/Polydopamine/Sr2+ Nanosheets on Titanium Surfaces for Enhanced Osteogenic Differentiation of Mesenchymal Stem Cells [Doi: 10.1016/j.carbon.2018.10.081]
        current?eye?research Detection of proteins associated with extracellular matrix regulation in the aqueous humour of patients with primary glaucoma. [Pubmed: 30994369]
        Journal of Proteomics Identification of urinary candidate biomarkers of cisplatin-induced nephrotoxicity in patients with carcinoma [Pubmed: 31629959]
        Acta Biomaterialia Inflammatory response to magnesium-based biodegradable implant materials [Pubmed: 31610341]
        Journal of Clinical Medicine Calcium Kidney Stones are Associated with Increased Risk of Carotid Atherosclerosis: the Link between Urinary Stone Risks, Carotid Intima-Media Thickness, and … [Pubmed: 32182704]
        EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES Febuxostat attenuates vascular calcification induced by vitamin D3 plus nicotine in rats [Pubmed: 33010420]
        [ENGINEERING OF BIOMATERIALS Osteoconductive potential of PLGA/bioglass composite biomaterials []
        Biomolecules Structural Constraint of Osteopontin Facilitates Efficient Binding to CD44 [34070790]
        Structural Changes and Detection of Liver Fibrosis¨CRelated Protein Levels in Coculture of Alveolar Echinococcosis-Protoscoleces and Human Hepatic Stellate cells []
        Parasit Vectors Structural changes and expression of hepatic fibrosis-related proteins in coculture of Echinococcus multilocularis protoscoleces and human hepatic stellate?… [34857049]
        Medicina (Kaunas) Plasma Concentrations of New Biochemical Markers of Atherosclerosis in Patients with Dyslipidemia—A Pilot Study [Pubmed:35743980]
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