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      1. 骨橋素(OPN)檢測試劑盒(酶聯免疫吸附試驗法)

        ELISA Kit for Osteopontin (OPN)

        SPP1; BNSP; BSPI; ETA1; Secreted Phosphoprotein 1; Bone Sialoprotein I; Early T-Lymphocyte Activation 1; Nephropontin; Urinary stone protein; Uropontin

        • 骨橋素(OPN)檢測試劑盒(酶聯免疫吸附試驗法) 產品包裝(模擬)
        • 骨橋素(OPN)檢測試劑盒(酶聯免疫吸附試驗法) 產品包裝(模擬)
        • 骨橋素(OPN)檢測試劑盒(酶聯免疫吸附試驗法) 實驗結果圖
        • SEA899Hu.jpg 標準曲線圖
        • Certificate 通過ISO 9001、ISO 13485質量體系認證





        樣本 回收率范圍(%) 平均回收率(%)
        serum(n=5) 91-99 96
        EDTA plasma(n=5) 96-103 101
        heparin plasma(n=5) 78-104 89


        精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
        批內差:取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續測定20 次,分別計算不同濃度樣本的平均值及SD值。
        批內差: CV<10%
        批間差: CV<12%



        樣本 1:2 1:4 1:8 1:16
        serum(n=5) 87-94% 78-98% 97-104% 79-105%
        EDTA plasma(n=5) 88-97% 93-101% 78-99% 83-101%
        heparin plasma(n=5) 84-97% 78-96% 91-99% 92-104%




        1. 實驗前標準品、試劑及樣本的準備;
        2. 加樣(標準品及樣本)100µL,37°C孵育1小時;
        3. 吸棄,加檢測溶液A100µL,37°C孵育1小時;
        4. 洗板3次;
        5. 加檢測溶液B100µL,37°C孵育30分鐘;
        6. 洗板5次;
        7. 加TMB底物90µL,37°C孵育10-20分鐘;
        8. 加終止液50µL,立即450nm讀數。




        編號 適用物種:Homo sapiens (Human,人) 應用(僅供研究使用,不用于臨床診斷!)
        EPA899Hu61 骨橋素(OPN)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
        RPA899Hu02 骨橋素(OPN)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
        APA899Hu61 骨橋素(OPN)活性蛋白 Cell?culture;?Activity?Assays.
        PAA899Hu06 骨橋素(OPN)多克隆抗體 WB; IHC; ICC; IP.
        PAA899Hu02 骨橋素(OPN)多克隆抗體 WB; IHC; ICC; IP.
        LAA899Hu71 骨橋素(OPN)多克隆抗體(生物素標記) WB; IHC; ICC.
        MAA899Hu22 骨橋素(OPN)單克隆抗體 WB; IHC; ICC; IP.
        SEA899Hu 骨橋素(OPN)檢測試劑盒(酶聯免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
        AEA899Hu 抗骨橋素抗體(Anti-OPN)檢測試劑盒(酶聯免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antibody Detection.
        SCA899Hu 骨橋素(OPN)檢測試劑盒(化學發光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
        LMA899Hu 骨橋素(OPN)單因子檢測試劑盒(流式熒光發光法) Magnetic Luminex Assay for Antigen Detection.
        KSA899Hu01 骨橋素(OPN)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) Main materials for "Do It (ELISA Kit) Yourself".


        雜志 參考文獻
        Journal of Biomedical Materials Research Part A Proliferation and osteogenesis of immortalized bone marrow-derived mesenchymal stem cells in porous polylactic glycolic acid scaffolds under perfusion culture [PubMed: 19280635]
        Osteoarthritis and Cartilage Elevated osteopontin level of synovial fluid and articular cartilage is associated with disease severity in knee osteoarthritis patients [PubMed: 19747583]
        The Journal of Rheumatology Thrombin-cleaved osteopontin levels in synovial fluid correlate with disease severity of knee osteoarthritis. [Jrheum: source]
        International Journal of Pharmaceutics Suppression of tumor growth in xenograft model mice by small interfering RNA targeting osteopontin delivery using biocompatible poly(amino ester) [ScienceDirect: S0378517312003742]
        European Cells and matreials The use of non-viral gene vectors for bioactive poly-(D, L-lactide) implant surfaces in bone tissue engineering [Ecmjournal: Source]
        Journal of Equine Veterinary Science Influence of Seminal Plasma Antioxidants and Osteopontin on Fertility of the Arabian Horse [ScienceDirect: Source]
        Basic &amp; Clinical Pharmacology &amp; Toxicology Treatment with Carnitine Enhances Bone Fracture Healing under Osteoporotic and/or Inflammatory Conditions [Wiley:Source]
        Journal of Cellular Biochemistry Tension Force-Induced ATP Promotes Osteogenesis Through P2X7 Receptor in Osteoblasts [Pubmed:24905552]
        Naunyn-Schmiedeberg&#039;s Archives of Pharmacology Treatment with α-lipoic acid enhances the bone healing after femoral fracture model of rats [Pubmed:25038619]
        J Endocrinol Invest.? The interplay among iron metabolism, endothelium and inflammatory cascade in dysmetabolic disorders [Pubmed:25245337]
        FEBS Letters Low-intensity pulsed ultrasound-induced ATP increases bone formation via the P2X7 receptor in osteoblast-like MC3T3-E1 cells [Pubmed:25542352]
        Gene Effects of magnesium degradation products on mesenchymal stem cell fate and osteoblastogenesis [PubMed: 26283150]
        Medicine (Baltimore) An Attempt to Evaluate Selected Aspects of “Bone–Fat Axis” Function in Healthy Individuals and Patients With Pancreatic Cancer [PubMed: 26266370]
        Journal of Biomaterials and Tissue Engineering Osteogenic Differentiation of Canine Adipose Stem Cells Cultured in Alginate-Fibrin-Based Hydrogel [Content: Asp]
        Primasari Osteopontin level correlates negatively with tumor shrinkage in neoadjuvant chemoradiation of locally advanced rectal cancer [Index.Php: Amor]
        Int J Biol Sci Defective Initiation of Liver Regeneration in Osteopontin-Deficient Mice after Partial Hepatectomy due to Insufficient Activation of IL-6/Stat3 Pathway [PubMed: 26327817]
        Journal of Kunming Medical University / Kunming Yike Daxue Xuebao Perioperative Clinical Investigation of Serum VEGF, CA19-9 and OPN Levels in Patients with Colorectal Carcinoma. [Ebscohost]
        Am J Pathol.? Serum Osteopontin as a Novel Biomarker for Muscle Regeneration in Duchenne Muscular Dystrophy. [Pubmed:26963343]
        circulation journal Stenotic Bicuspid and Tricuspid Aortic Valves - Micro-Computed Tomography and Biological Indices of Calcification. [pubmed:28344201]
        Journal of Cardiovascular Electrophysiology Effect of Cardiac Resynchronization Therapy on Myocardial Fibrosis and Relevant Cytokines in a Canine Model With Experimental Heart Failure. [pubmed:28127817]
        American Journal of Translational Research Osteopontin stimulates matrix metalloproteinase expression through the nuclear factor-κB signaling pathway in rat temporomandibular joint and condylar chondrocytes. [pubmed:28337262]
        Diabetes Care Targeted Microbiome Intervention by Microencapsulated Delayed-Release Niacin Beneficially Affects Insulin Sensitivity in Humans [pubmed:29212824]
        Biomedicine & pharmacotherapy Icaritin induces MC3T3-E1 subclone14 cell differentiation through estrogen receptor-mediated ERK1/2 and p38 signaling activation. [pubmed:28742995]
        Journal of?Alzheimers?Disease Urine-Based Biomarkers for Alzheimer's Disease Identified Through Coupling Computational and Experimental Methods [Pubmed:30040720]
        Indian?Journal?of?Nephrology Osteopontin, cardiovascular risk factors and carotid intima-Media thickness in chronic kidney disease [id:233806]
        Carbon In Situ Self-Assembly of Graphene Oxide/Polydopamine/Sr2+ Nanosheets on Titanium Surfaces for Enhanced Osteogenic Differentiation of Mesenchymal Stem Cells [Doi: 10.1016/j.carbon.2018.10.081]
        current?eye?research Detection of proteins associated with extracellular matrix regulation in the aqueous humour of patients with primary glaucoma. [Pubmed: 30994369]
        Journal of Proteomics Identification of urinary candidate biomarkers of cisplatin-induced nephrotoxicity in patients with carcinoma [Pubmed: 31629959]
        Acta Biomaterialia Inflammatory response to magnesium-based biodegradable implant materials [Pubmed: 31610341]
        Journal of Clinical Medicine Calcium Kidney Stones are Associated with Increased Risk of Carotid Atherosclerosis: the Link between Urinary Stone Risks, Carotid Intima-Media Thickness, and … [Pubmed: 32182704]
        EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES Febuxostat attenuates vascular calcification induced by vitamin D3 plus nicotine in rats [Pubmed: 33010420]
        [ENGINEERING OF BIOMATERIALS Osteoconductive potential of PLGA/bioglass composite biomaterials []
        Biomolecules Structural Constraint of Osteopontin Facilitates Efficient Binding to CD44 [34070790]
        Structural Changes and Detection of Liver Fibrosis¨CRelated Protein Levels in Coculture of Alveolar Echinococcosis-Protoscoleces and Human Hepatic Stellate cells []
        Parasit Vectors Structural changes and expression of hepatic fibrosis-related proteins in coculture of Echinococcus multilocularis protoscoleces and human hepatic stellate?… [34857049]
        Medicina (Kaunas) Plasma Concentrations of New Biochemical Markers of Atherosclerosis in Patients with Dyslipidemia—A Pilot Study [Pubmed:35743980]