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      1. 腎素(REN)檢測試劑盒(酶聯免疫吸附試驗法)

        ELISA Kit for Renin (REN)

        angiotensinogenase

        • 腎素(REN)檢測試劑盒(酶聯免疫吸附試驗法) 產品包裝(模擬)
        • 腎素(REN)檢測試劑盒(酶聯免疫吸附試驗法) 產品包裝(模擬)
        • 腎素(REN)檢測試劑盒(酶聯免疫吸附試驗法) 實驗結果圖
        • SEA889Hu.jpg 標準曲線圖
        • Certificate 通過ISO 9001、ISO 13485質量體系認證

        特異性

        本試劑盒用于檢測腎素(REN),經檢測與其它相似物質無明顯交叉反應。
        由于受到技術及樣本來源的限制,不可能完成對所有相關或相似物質交叉反應檢測,因此本試劑盒有可能與未經檢測的其它物質有交叉反應。

        回收率

        分別于定值血清及血漿樣本中加入一定量的腎素(REN)(加標樣品),重復測定并計算其均值,回收率為測定值與理論值的比率。

        樣本 回收率范圍(%) 平均回收率(%)
        serum(n=5) 80-88 84
        EDTA plasma(n=5) 81-101 97
        heparin plasma(n=5) 95-103 101

        精密度

        精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
        批內差:取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續測定20 次,分別計算不同濃度樣本的平均值及SD值。
        批間差:選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定,每個樣本使用同一試劑盒重復測定8次,分別計算不同濃度樣本的平均值及SD值。
        批內差: CV<10%
        批間差: CV<12%

        線性

        在定值血清及血漿樣本內加入適量的腎素(REN),并倍比稀釋成1:2,1:4,1:8,1:16的待測樣本,線性范圍即為稀釋后樣本中腎素(REN)含量的測定值與理論值的比率。

        樣本 1:2 1:4 1:8 1:16
        serum(n=5) 79-90% 80-104% 86-102% 85-98%
        EDTA plasma(n=5) 93-105% 85-101% 90-98% 87-94%
        heparin plasma(n=5) 90-103% 90-97% 99-105% 98-105%

        穩定性

        經測定,試劑盒在有效期內按推薦溫度保存,其活性降低率小于5%。
        為減小外部因素對試劑盒破壞前后檢測值的影響,實驗室的環境條件需盡量保持一致,尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

        實驗流程

        1. 實驗前標準品、試劑及樣本的準備;
        2. 加樣(標準品及樣本)100µL,37°C孵育1小時;
        3. 吸棄,加檢測溶液A100µL,37°C孵育1小時;
        4. 洗板3次;
        5. 加檢測溶液B100µL,37°C孵育30分鐘;
        6. 洗板5次;
        7. 加TMB底物90µL,37°C孵育10-20分鐘;
        8. 加終止液50µL,立即450nm讀數。

        實驗原理

        將腎素(REN)抗體包被于96孔微孔板中,制成固相載體,向微孔中分別加入標準品或標本,其中的腎素(REN)與連接于固相載體上的抗體結合,然后加入生物素化的腎素(REN)抗體,將未結合的生物素化抗體洗凈后,加入HRP標記的親和素,再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉化成藍色,并在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中的腎素(REN)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值),計算樣品濃度。

        相關產品

        編號 適用物種:Homo sapiens (Human,人) 應用(僅供研究使用,不用于臨床診斷!)
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        參考文獻

        雜志 參考文獻
        Hypertension Prenatal overexposure to glucocorticoids programs renal 11β-hydroxysteroid dehydrogenase type 2 expression and salt-sensitive hypertension in the rat [PubMed: 21045727]
        The Journal of Physiology Recombinant human erythropoietin in humans down-regulates proximal renal tubular reabsorption and causes a fall in glomerular filtration rate [Physoc: 194241]
        The Journal of Physiology Erythropoietin down‐regulates proximal renal tubular reabsorption and causes a fall in glomerular filtration rate in humans [PubMed: 20724370]
        Pathobiology Anti-Angiotensin and Hypoglycemic Treatments Suppress Liver Metastasis of Colon Cancer Cells [Karger: 000330169]
        Journal of Cardiovascular Pharmacology The Angiotensin Receptor Blocker, Azilsartan Medoxomil (TAK-491), Suppresses Vascular Wall Expression of Plasminogen Activator Inhibitor Type-I Protein Potentially Facilitating the Stabilization of Atherosclerotic Plaques [LWW: 08000]
        Kidney International Multiple and large simple renal cysts are associated with prehypertension and hypertension. [Pubmed: 23389415]
        Human Physiology Estimation of the parameters of the renin-angiotensin-aldosterone system, water-electrolyte metabolism, and endothelial function in newborns of women with chronic hypertension [Springer:Source]
        Journal of Pharmacological Sciences Organ-Specific Activation of the Gastric Branch of the Efferent Vagus Nerve by Ghrelin in Urethane-Anesthetized Rats [Pubmed:24366191]
        Liver International VSL#3 probiotic treatment decreases bacterial translocation in rats with carbon tetrachloride-induced cirrhosis [Pubmed:24750552]
        Gynecological Endocrinology Immunological and hemostasiological disorders in women with ovarian hyperstimulation syndrome [Pubmed:25200828]
        Oxidative Medicine and Cellular Longevity Age-Associated Changes in the Vascular Renin-Angiotensin System in Mice [Pubmed:27200147]
        PLoS One Ablation of Potassium-Chloride Cotransporter Type 3 (Kcc3) in Mouse Causes Multiple Cardiovascular Defects and Isosmotic Polyuria [Pubmed:27166674]
        PLOS?ONE Reduced?Expression?of the?Extracellular?Calcium-Sensing?Receptor?(CaSR) Is?Associated?withActivation?of the?Renin-Angiotensin?System?(RAS) to Promote Vascular Remodeling in the Pathogenesis of Essential Hypertension. [pubmed:27391973]
        Heart Lung Circ. Efficacy and Safety of Renal Sympathetic Denervation on Dogs with Pressure Overload-Induced Heart Failure. [pubmed:27555054]
        European Journal of Pharmaceutical Sciences Sacubitril/valsartan (LCZ696) Significantly Reduces Aldosterone and Increases cGMP Circulating Levels in a Canine Model of RAAS Activation [Doi: 10.1101/435560]
        Nutrition Gut Dysbiosis Contribute to High Fructose Induced Salt Sensitive Hypertension In Sprague-Dawley Rats []
        ACTA PHARMACOLOGICA SINICA The vascular endothelial growth factor trap aflibercept induces vascular dysfunction and hypertension via attenuation of eNOS/NO signaling in mice [33303990]
        Hypertens Res Association of Ang-(1¨C7) and des-Arg 9 BK as new biomarkers of obesity and cardiometabolic risk factors in adolescents [33568792]
        Phosphorylation of ?21-integrin in juxtaglomerular cells helps control blood pressure during the progression of diabetic nephropathy []
        Bedeutung des intrarenalen Renin-Angiotensin-Systems f¨1r die Entwicklung der chronischen Nierenkrankheit []
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