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      1. P選擇素(SELP)檢測試劑盒(酶聯免疫吸附試驗法)

        ELISA Kit for P-Selectin (SELP)

        CD62P; Selectin, Platelet; PSEL; GMP140; LECAM3; GRMP; GMRP; PADGEM; Leukocyte-endothelial cell adhesion molecule 3; Platelet activation dependent granule-external membrane

        • P選擇素(SELP)檢測試劑盒(酶聯免疫吸附試驗法) 產品包裝(模擬)
        • P選擇素(SELP)檢測試劑盒(酶聯免疫吸附試驗法) 產品包裝(模擬)
        • P選擇素(SELP)檢測試劑盒(酶聯免疫吸附試驗法) 實驗結果圖
        • SEA569Hu.jpg 標準曲線圖
        • Certificate 通過ISO 9001、ISO 13485質量體系認證

        特異性

        本試劑盒用于檢測P選擇素(SELP),經檢測與其它相似物質無明顯交叉反應。
        由于受到技術及樣本來源的限制,不可能完成對所有相關或相似物質交叉反應檢測,因此本試劑盒有可能與未經檢測的其它物質有交叉反應。

        回收率

        分別于定值血清及血漿樣本中加入一定量的P選擇素(SELP)(加標樣品),重復測定并計算其均值,回收率為測定值與理論值的比率。

        樣本 回收率范圍(%) 平均回收率(%)
        serum(n=5) 98-105 102
        EDTA plasma(n=5) 85-96 92
        heparin plasma(n=5) 90-102 98

        精密度

        精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
        批內差:取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續測定20 次,分別計算不同濃度樣本的平均值及SD值。
        批間差:選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定,每個樣本使用同一試劑盒重復測定8次,分別計算不同濃度樣本的平均值及SD值。
        批內差: CV<10%
        批間差: CV<12%

        線性

        在定值血清及血漿樣本內加入適量的P選擇素(SELP),并倍比稀釋成1:2,1:4,1:8,1:16的待測樣本,線性范圍即為稀釋后樣本中P選擇素(SELP)含量的測定值與理論值的比率。

        樣本 1:2 1:4 1:8 1:16
        serum(n=5) 96-105% 90-104% 88-103% 85-101%
        EDTA plasma(n=5) 98-105% 98-105% 91-99% 97-104%
        heparin plasma(n=5) 95-102% 92-101% 78-98% 86-94%

        穩定性

        經測定,試劑盒在有效期內按推薦溫度保存,其活性降低率小于5%。
        為減小外部因素對試劑盒破壞前后檢測值的影響,實驗室的環境條件需盡量保持一致,尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

        實驗流程

        1. 實驗前標準品、試劑及樣本的準備;
        2. 加樣(標準品及樣本)100µL,37°C孵育1小時;
        3. 吸棄,加檢測溶液A100µL,37°C孵育1小時;
        4. 洗板3次;
        5. 加檢測溶液B100µL,37°C孵育30分鐘;
        6. 洗板5次;
        7. 加TMB底物90µL,37°C孵育10-20分鐘;
        8. 加終止液50µL,立即450nm讀數。

        實驗原理

        將P選擇素(SELP)抗體包被于96孔微孔板中,制成固相載體,向微孔中分別加入標準品或標本,其中的P選擇素(SELP)與連接于固相載體上的抗體結合,然后加入生物素化的P選擇素(SELP)抗體,將未結合的生物素化抗體洗凈后,加入HRP標記的親和素,再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉化成藍色,并在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中的P選擇素(SELP)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值),計算樣品濃度。

        相關產品

        編號 適用物種:Homo sapiens (Human,人) 應用(僅供研究使用,不用于臨床診斷!)
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        參考文獻

        雜志 參考文獻
        Journalof Vascular Research Effect of Hirulog-Like Peptide on Balloon Catheter Injury-Induced Neointimal Formation in Femoral Arteries of Minipigs and Relationship with Inflammatory Mediators [Karger: 000257340]
        Basic Research in Cardiology Secretome of apoptotic peripheral blood cells (APOSEC) attenuates microvascular obstruction in a porcine closed chest reperfused acute myocardial infarction model: role of platelet aggregation and vasodilation [PubMed: 22899170]
        BioMed research international Endothelial gene expression and molecular changes in response to radiosurgery in in vitro and in vivo models of cerebral arteriovenous malformations [Pubmed: 24199192]
        Free Radical Biology and Medicine Involvement of nitric oxide with activation of Toll-like receptor 4 signaling in mice with dextran sodium sulfate-induced colitis [Pubmed:24992835]
        Materials Science and Engineering: C MRI-compatible Nb–60Ta–2Zr alloy used for vascular stents: Haemocompatibility and its correlation with protein adsorption [Pubmed:25063132]
        Blood Coagul Fibrinolysis.? Chemical sympathectomy attenuates inflammation, glycocalyx shedding and coagulation disorders in rats with acute traumatic coagulopathy. [Pubmed:25325345]
        Inflammation.? Systemic and Flap Inflammatory Response Associates with Thrombosis in Flap Venous Crisis [Pubmed:25448261]
        Endocrine Effects of glucagon-like peptide-1 on advanced glycation endproduct-induced aortic endothelial dysfunction in streptozotocin-induced diabetic rats: possible roles of Rho kinase- and AMP kinase-mediated nuclear factor κB signaling pathways [Pubmed:26758998]
        Microbial Pathogenesis Protective effects of sinomenine against LPS-induced inflammation in piglets. [pubmed:28757275]
        Nutrition?&?Metabolism L-Carnitine Supplementation Increases Trimethylamine-N-Oxide but not Markers of Atherosclerosis in Healthy Aged Women [Pubmed: 30485835]
        Available at SSRN 3335365? Evaluating the Platelet Activation Related to the Degradation of Biomaterials by Scheme of Molecular Markers []
        Ecotoxicology?and?Environmental?Safety Molecular mechanisms of lead-induced changes of selenium status in mice livers through interacting with selenoprotein P []
        Thrombosis Research Feasibility study of use of rabbit blood to evaluate platelet activation by medical devices [Pubmed: 31838449]
        JOURNAL OF CONTROLLED RELEASE Polycaprolactone vascular graft with epigallocatechin gallate embedded sandwiched layer-by-layer functionalization for enhanced antithrombogenicity and anti … [Pubmed: 31982435]
        OXIDATIVE MEDICINE AND CELLULAR LONGEVITY The Inhibition of P-Selectin Reduced Severe Acute Lung Injury in Immunocompromised Mice [Pubmed: 32377309]
        blood advances The binding of autotaxin to integrins mediates hyperhomocysteinemia-potentiated platelet activation and thrombosis [34559203]
        Vaccine The effect of ChAdOx1 nCov-19 vaccine on arterial thrombosis development and platelet aggregation in female rats [Pubmed:35183388]
        Int J Biol Macromol The inhibition of TRIM35-mediated TIGAR ubiquitination enhances mitochondrial fusion and alleviates renal ischemia-reperfusion injury [Pubmed:35421414]
        Experimental and Therapeutic Medicine Construction of a rabbit model with vinorelbine administration via peripherally inserted central catheter and dynamic monitoring of changes in phlebitis and thrombosis [Pubmed:35126715]
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        亚洲精品国产情侣AV在线,日韩精品一区二区三区色欲AV,免费看高清黄A级毛片