亚洲精品国产情侣AV在线,日韩精品一区二区三区色欲AV,免费看高清黄A级毛片

  • <object id="jwsct"><legend id="jwsct"></legend></object>
    <object id="jwsct"></object>
  • <s id="jwsct"></s>
      1. 血紅素氧合酶1(HO1)檢測試劑盒(酶聯免疫吸附試驗法)

        ELISA Kit for Heme Oxygenase 1 (HO1)

        HMOX1; Hsp32; HMOX1D

        • 血紅素氧合酶1(HO1)檢測試劑盒(酶聯免疫吸附試驗法) 產品包裝(模擬)
        • 血紅素氧合酶1(HO1)檢測試劑盒(酶聯免疫吸附試驗法) 產品包裝(模擬)
        • 血紅素氧合酶1(HO1)檢測試劑盒(酶聯免疫吸附試驗法) 實驗結果圖
        • SEA584Mu.jpg 標準曲線圖
        • Certificate 通過ISO 9001、ISO 13485質量體系認證

        特異性

        本試劑盒用于檢測血紅素氧合酶1(HO1),經檢測與其它相似物質無明顯交叉反應。
        由于受到技術及樣本來源的限制,不可能完成對所有相關或相似物質交叉反應檢測,因此本試劑盒有可能與未經檢測的其它物質有交叉反應。

        回收率

        分別于定值血清及血漿樣本中加入一定量的血紅素氧合酶1(HO1)(加標樣品),重復測定并計算其均值,回收率為測定值與理論值的比率。

        樣本 回收率范圍(%) 平均回收率(%)
        serum(n=5) 88-96 91
        EDTA plasma(n=5) 78-91 81
        heparin plasma(n=5) 80-92 85

        精密度

        精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
        批內差:取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續測定20 次,分別計算不同濃度樣本的平均值及SD值。
        批間差:選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定,每個樣本使用同一試劑盒重復測定8次,分別計算不同濃度樣本的平均值及SD值。
        批內差: CV<10%
        批間差: CV<12%

        線性

        在定值血清及血漿樣本內加入適量的血紅素氧合酶1(HO1),并倍比稀釋成1:2,1:4,1:8,1:16的待測樣本,線性范圍即為稀釋后樣本中血紅素氧合酶1(HO1)含量的測定值與理論值的比率。

        樣本 1:2 1:4 1:8 1:16
        serum(n=5) 99-105% 88-105% 85-97% 94-103%
        EDTA plasma(n=5) 78-94% 81-101% 88-98% 83-96%
        heparin plasma(n=5) 88-103% 80-103% 91-101% 99-105%

        穩定性

        經測定,試劑盒在有效期內按推薦溫度保存,其活性降低率小于5%。
        為減小外部因素對試劑盒破壞前后檢測值的影響,實驗室的環境條件需盡量保持一致,尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

        實驗流程

        1. 實驗前標準品、試劑及樣本的準備;
        2. 加樣(標準品及樣本)100µL,37°C孵育1小時;
        3. 吸棄,加檢測溶液A100µL,37°C孵育1小時;
        4. 洗板3次;
        5. 加檢測溶液B100µL,37°C孵育30分鐘;
        6. 洗板5次;
        7. 加TMB底物90µL,37°C孵育10-20分鐘;
        8. 加終止液50µL,立即450nm讀數。

        實驗原理

        將血紅素氧合酶1(HO1)抗體包被于96孔微孔板中,制成固相載體,向微孔中分別加入標準品或標本,其中的血紅素氧合酶1(HO1)與連接于固相載體上的抗體結合,然后加入生物素化的血紅素氧合酶1(HO1)抗體,將未結合的生物素化抗體洗凈后,加入HRP標記的親和素,再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉化成藍色,并在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中的血紅素氧合酶1(HO1)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值),計算樣品濃度。

        相關產品

        編號 適用物種:Mus musculus (Mouse,小鼠) 應用(僅供研究使用,不用于臨床診斷!)
        RPA584Mu01 血紅素氧合酶1(HO1)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
        RPA584Mu02 血紅素氧合酶1(HO1)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
        PAA584Mu02 血紅素氧合酶1(HO1)多克隆抗體 WB; IHC; ICC; IP.
        PAA584Mu01 血紅素氧合酶1(HO1)多克隆抗體 WB; IHC; ICC; IP.
        LAA584Mu71 血紅素氧合酶1(HO1)多克隆抗體(生物素標記) WB; IHC; ICC.
        SEA584Mu 血紅素氧合酶1(HO1)檢測試劑盒(酶聯免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
        LMA584Mu 血紅素氧合酶1(HO1)單因子檢測試劑盒(流式熒光發光法) Magnetic Luminex Assay for Antigen Detection.

        參考文獻

        雜志 參考文獻
        The American Journal of Pathology Characterization of the Intrarenal Renin-Angiotensin System in Experimental Alport Syndrome [PubMed: 25777063]
        BioMed Research International Serum Heme Oxygenase-1 and BMP-7 Are Potential Biomarkers for Bone Metabolism in Patients with Rheumatoid Arthritis and Ankylosing Spondylitis [Pubmed:27314037]
        Cancer Medicine Circulating CD14+HLA-DR-/low myeloid-derived suppressor cells in leukemia patients with allogeneic hematopoietic stem cell transplantation: novel clinical potential strategies for the prevention and cellular therapy of graft-versus-host disease [Pubmed:27109254]
        Redox Report: Communications in Free Radical Research Decreased expression of heme oxygenase is associated with depressive symptoms and may contribute to depressive and hypertensive comorbidity [Pubmed:26824276]
        Canadian Journal of Physiology and Pharmacology Sulforaphane protects against sodium valproate–induced acute liver injury [pubmed:28112972]
        Peptides. Carnosine ameliorates cognitive deficits in streptozotocin-induced diabetic rats: Possible involved mechanisms. [pubmed:27777064]
        european journal of pharmacology S-allyl cysteine ameliorates cognitive deficits in streptozotocin-diabetic rats via suppression of oxidative stress, inflammation, and acetylcholinesterase. [pubmed:27887948]
        brain research Ellagic acid exerts protective effect in intrastriatal 6-hydroxydopamine rat model of Parkinson's disease: Possible involvement of ERβ/Nrf2/HO-1 signaling. [pubmed:28238669]
        biomedicine & pharmacotherapy Citrus leaf extract reduces blood pressure and vascular damage in repeatedly heated palm oil diet-Induced hypertensive rats. [pubmed:28068636]
        Inflammopharmacology. Implication of Nrf2/HO-1 pathway in the coloprotective effect of coenzyme Q10 against experimentally induced ulcerative colitis. [pubmed:28050757]
        Environmental Toxicology and Pharmacology Ameliorative potential of linagliptin and/or calcipotriol on bleomycin-induced lung fibrosis: In vivo and in vitro study [pubmed:28192751]
        Journal of Microbiology, Immunology and Infection Identification of a panel of serum protein markers in early stage of sepsis and its validation in a cohort of patients [pubmed:28655573]
        Nutrition & Metabolism Hepatic, adipocyte, enteric and pancreatic hormones: response to dietary macronutrient composition and relationship with metabolism [pubmed:28694840]
        Biomedicine & Pharmacotherapy Ameliorative potential of sitagliptin and/or resveratrol on experimentallyinduced clear cell renal cell carcinoma [pubmed:29101811]
        Acta Paediatrica Serum complement factor 5a levels are associated with nonalcoholic fatty liver disease in obese children [DOI:?10.1111/apa.14106]
        Naunyn-Schmiedeberg's Arch Pharmacol Quercetin and tin protoporphyrin attenuate hepatic ischemia reperfusion injury: role of HO-1 [pubmed:28589248]
        Asian Pacific Journal of Cancer Prevention Ameliorative Effect of Coenzyme Q10 and/or Candesartan on Carboplatin-Induced Nephrotoxicity: Roles of Apoptosis, Transforming Growth Factor-Β1, Nuclear Factor Kappa-B And The Nrf2/HO-1 Pathway [pubmed:28670881]
        Biomedicine & pharmacotherapy Berberine ameliorates intrahippocampal kainate-induced status epilepticus and consequent epileptogenic process in the rat: Underlying mechanisms [pubmed:28061403]
        Talanta Simultaneous accurate quantification of HO-1, CD39, and CD73 in human calcified aortic valves using multiple enzyme digestion–filter aided sample pretreatment?… [10.1016:j.talanta.2018.01.044]
        Pharmacological Reports The Association between Inflammatory Markers (iNOS, HO-1, IL-33, MIP-1β) and Depression with and without Posttraumatic Stress Disorder [10.1016:j.pharep.2018.06.001]
        Physiology &?Behavior? Linagliptin potentiates the effect of l-dopa on the behavioural, biochemical and immunohistochemical changes in experimentally-induced Parkinsonism: Role of toll?… [Pubmed:29410018]
        Journal of?Infection?and Chemotherapy Targeting the proinflammatory cytokines, oxidative stress, apoptosis and TGF-β1/STAT-3 signaling by irbesartan to ameliorate doxorubicin-induced hepatotoxicity [Pubmed:29753615]
        American Institute of Physics Anti-hemolytic and anti-inflammatory activities of the methanolic extract of (P.Beauv.) Briq. leaves in 2-butoxyethanol-hemolytic induced?… [10.1063:1.5033415]
        Iranian?Journal?of?Basic?Medical Sciences Tempol relieves lung injury in a rat model of chronic intermittent hypoxia via suppression of inflammation and oxidative stress [Doi: 10.22038/IJBMS.2018.31716.7714]
        Biomedicine & Pharmacotherapy Targeting Nrf2/HO-1 signaling by crocin: Role in attenuation of AA-induced ulcerative colitis in rats [Pubmed: 30530041]
        Experimental Parasitology Sodium arsenite augments sensitivity of Echinococcus granulosus protoscoleces to albendazole [Pubmed: 30790573]
        JGH OPEN Cilostazol against 2, 4, 6‐trinitrobenzene sulfonic acid‐induced colitis: Effect on tight junction, inflammation, and apoptosis []
        Journal of Food Biochemistry Vanillic acid alleviates palmitic acid‐induced oxidative stress in human umbilical vein endothelial cells via Adenosine Monophosphate‐Activated Protein Kinase?… []
        Experimental and Therapeutic Medicine Effect of heme oxygenase 1 and renin/prorenin receptor on oxidized low?density lipoprotein?induced human umbilical vein endothelial cells []
        Life Sciences Crocin attenuates lung inflammation and pulmonary vascular dysfunction in a rat model of bleomycin-induced pulmonary fibrosis [Pubmed: 31465731]
        Respiratory physiology & neurobiology Targeting oxidative stress, proinflammatory cytokines, apoptosis and toll like receptor 4 by empagliflozin to ameliorate bleomycin-induced lung fibrosis [Pubmed: 31600583]
        Life Sci The protective effect of Lactobacillus versus 5-aminosalicylic acid in ulcerative colitis model by modulation of gut microbiota and Nrf2/Ho-1 pathway [Pubmed: 32526285]
        JAMA Surgery Intraoperative dexmedetomidine for prevention of postoperative delirium in elderly patients with mild cognitive impairment [Pubmed: 28593326]
        JOURNAL OF PHARMACY AND PHARMACOLOGY Isoliquiritigenin attenuates inflammation and modulates Nrf2/caspase-3 signalling in STZ-induced aortic injury [33793806]
        Exp Parasitol In vitro protoscolicidal effects of lithocholic acid on protoscoleces of Echinococcus granulosus and its mechanism [Pubmed:35594934]
        Free Radical Biology and Medicine Flavonoid 4, 4′-dimethoxychalcone induced ferroptosis in cancer cells by synergistically activating Keap1/Nrf2/HMOX1 pathway and inhibiting FECH [Pubmed:35697292]
        留言咨詢
        亚洲精品国产情侣AV在线,日韩精品一区二区三区色欲AV,免费看高清黄A级毛片