亚洲精品国产情侣AV在线,日韩精品一区二区三区色欲AV,免费看高清黄A级毛片

  • <object id="jwsct"><legend id="jwsct"></legend></object>
    <object id="jwsct"></object>
  • <s id="jwsct"></s>
      1. 脂蛋白關聯磷脂酶A2(LpPLA2)檢測試劑盒(酶聯免疫吸附試驗法)

        ELISA Kit for Phospholipase A2 Group VII (LpPLA2)

        PLA2G7; PAF-AH; PAFAH; Lp-PLA2; LDL-PLA2; Platelet Activating Factor Acetylhydrolase,Plasma; Phospholipase A2,Group VII; LDL-associated phospholipase A2; Phospholipase A2, Lipoprotein Associated

        • 脂蛋白關聯磷脂酶A2(LpPLA2)檢測試劑盒(酶聯免疫吸附試驗法) 產品包裝(模擬)
        • 脂蛋白關聯磷脂酶A2(LpPLA2)檢測試劑盒(酶聯免疫吸附試驗法) 產品包裝(模擬)
        • 脂蛋白關聯磷脂酶A2(LpPLA2)檢測試劑盒(酶聯免疫吸附試驗法) 實驗結果圖
        • SEA867Mu.jpg 標準曲線圖
        • Certificate 通過ISO 9001、ISO 13485質量體系認證

        特異性

        本試劑盒用于檢測脂蛋白關聯磷脂酶A2(LpPLA2),經檢測與其它相似物質無明顯交叉反應。
        由于受到技術及樣本來源的限制,不可能完成對所有相關或相似物質交叉反應檢測,因此本試劑盒有可能與未經檢測的其它物質有交叉反應。

        回收率

        分別于定值血清及血漿樣本中加入一定量的脂蛋白關聯磷脂酶A2(LpPLA2)(加標樣品),重復測定并計算其均值,回收率為測定值與理論值的比率。

        樣本 回收率范圍(%) 平均回收率(%)
        serum(n=5) 92-102 98
        EDTA plasma(n=5) 94-105 99
        heparin plasma(n=5) 83-91 87

        精密度

        精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
        批內差:取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續測定20 次,分別計算不同濃度樣本的平均值及SD值。
        批間差:選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定,每個樣本使用同一試劑盒重復測定8次,分別計算不同濃度樣本的平均值及SD值。
        批內差: CV<10%
        批間差: CV<12%

        線性

        在定值血清及血漿樣本內加入適量的脂蛋白關聯磷脂酶A2(LpPLA2),并倍比稀釋成1:2,1:4,1:8,1:16的待測樣本,線性范圍即為稀釋后樣本中脂蛋白關聯磷脂酶A2(LpPLA2)含量的測定值與理論值的比率。

        樣本 1:2 1:4 1:8 1:16
        serum(n=5) 89-97% 89-96% 98-105% 87-103%
        EDTA plasma(n=5) 84-99% 93-102% 87-97% 80-93%
        heparin plasma(n=5) 78-97% 79-101% 88-96% 83-92%

        穩定性

        經測定,試劑盒在有效期內按推薦溫度保存,其活性降低率小于5%。
        為減小外部因素對試劑盒破壞前后檢測值的影響,實驗室的環境條件需盡量保持一致,尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

        實驗流程

        1. 實驗前標準品、試劑及樣本的準備;
        2. 加樣(標準品及樣本)100µL,37°C孵育1小時;
        3. 吸棄,加檢測溶液A100µL,37°C孵育1小時;
        4. 洗板3次;
        5. 加檢測溶液B100µL,37°C孵育30分鐘;
        6. 洗板5次;
        7. 加TMB底物90µL,37°C孵育10-20分鐘;
        8. 加終止液50µL,立即450nm讀數。

        實驗原理

        將脂蛋白關聯磷脂酶A2(LpPLA2)抗體包被于96孔微孔板中,制成固相載體,向微孔中分別加入標準品或標本,其中的脂蛋白關聯磷脂酶A2(LpPLA2)與連接于固相載體上的抗體結合,然后加入生物素化的脂蛋白關聯磷脂酶A2(LpPLA2)抗體,將未結合的生物素化抗體洗凈后,加入HRP標記的親和素,再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉化成藍色,并在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中的脂蛋白關聯磷脂酶A2(LpPLA2)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值),計算樣品濃度。

        相關產品

        編號 適用物種:Mus musculus (Mouse,小鼠) 應用(僅供研究使用,不用于臨床診斷!)
        RPA867Mu03 脂蛋白關聯磷脂酶A2(LpPLA2)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
        RPA867Mu04 脂蛋白關聯磷脂酶A2(LpPLA2)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
        RPA867Mu01 脂蛋白關聯磷脂酶A2(LpPLA2)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
        RPA867Mu02 脂蛋白關聯磷脂酶A2(LpPLA2)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
        PAA867Mu02 脂蛋白關聯磷脂酶A2(LpPLA2)多克隆抗體 WB; IHC; ICC; IP.
        PAA867Mu01 脂蛋白關聯磷脂酶A2(LpPLA2)多克隆抗體 WB; IHC; ICC; IP.
        LAA867Mu81 脂蛋白關聯磷脂酶A2(LpPLA2)多克隆抗體(異硫氰酸熒光素標記) WB; IHC; ICC; IF.
        LAA867Mu71 脂蛋白關聯磷脂酶A2(LpPLA2)多克隆抗體(生物素標記) WB; IHC; ICC.
        SEA867Mu 脂蛋白關聯磷脂酶A2(LpPLA2)檢測試劑盒(酶聯免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
        LMA867Mu 脂蛋白關聯磷脂酶A2(LpPLA2)單因子檢測試劑盒(流式熒光發光法) Magnetic Luminex Assay for Antigen Detection.

        參考文獻

        雜志 參考文獻
        Science & Sports The effects of different intensity walking programs on serum blood lipids, high-sensitive C-reactive protein, and lipoprotein-associated phospholipase A2 in premenopausal women [ScienceDirect: S0765159710000262]
        Metabolic Syndrome and Related Disorders Association of Serum Lipoprotein-Associated Phospholipase A2 Level with Nonalcoholic Fatty Liver Disease [Wiley: source]
        34 Effects of Pycnogenol on endothelial function in patients with stable coronary artery disease: a double-blind, randomized, placebo-controlled, cross-over study [PubMed: 22240497]
        PLoS ONE Effects of Lipid-Lowering Drugs on Irisin in Human Subjects In Vivo and in Human Skeletal Muscle Cells Ex Vivo [PubMed: PMC3759413]
        China Pharmacy Influence of Tongfengtai Granules on Inflammatory Factor in Acute Gouty Arthritis Model Rats [Source]
        Atherosclerosis Everolimus therapy is associated with reduced lipoprotein-associated phospholipase A2 (Lp-Pla2) activity and oxidative stress in heart transplant recipients. [Pubmed: 23958269]
        Journal of Diabetes Research Lipoprotein-Associated Phospholipase A2 Mass Level Is Increased in Elderly Subjects with Type 2 Diabetes Mellitus [Hindawi: 278063]
        Health The Role of Asymmetric Dimethylarginine and Lipoprotein Associated Phospholipase A2 in Children and Adolescents with Dyslipidemia [Scirp:Source]
        Biology Bulletin In vitro lipid-lowering and fibrinolytic effects of regulatory leucine-containing glyprolines in human blood [Springer:Source]
        Gynecol Endocrinol. Lipoprotein-associated phospholipase A2 and AGEs are associated with cardiovascular risk factors in women with history of gestational diabetes mellitus. [Pubmed:24397392]
        Journal of Diabetes Research Lipoprotein-Associated Phospholipase A 2 Mass Level Is Increased in Elderly Subjects with Type 2 Diabetes Mellitus [Pubmed:24818163]
        Metabolism. Effects of sitagliptin therapy on markers of low-grade inflammation and cell adhesion molecules in patients with type 2 diabetes [Pubmed:25034387]
        J Am Heart Assoc Effect of Extended‐Release Niacin on High‐Density Lipoprotein (HDL) Functionality, Lipoprotein Metabolism, and Mediators of Vascular Inflammation in Statin‐Treated Patients [PubMed: 26374297]
        Czecho-Slovak Dermatology / Cesko-Slovenska Dermatologie V [Ebscohost]
        Endocrinol Metab (Seoul). Effect of Pitavastatin Treatment on ApoB-48 and Lp-PLA2?in Patients with Metabolic Syndrome: Substudy of PROspective Comparative Clinical Study Evaluating the Efficacy and Safety of PITavastatin in Patients with Metabolic Syndrome [pmc:PMC4803547]
        Clin Pharmacol Ther.? Effect of dipyridamole on myocardial reperfusion injury: A double‐blind randomized controlled trial in patients undergoing elective coronary artery bypass surgery [Pubmed:25773594]
        Lipids in Health and Disease Effect of intensive insulin treatment on plasma levels of lipoprotein-associated phospholipase A 2 and secretory phospholipase A 2 in patients with newly … [articles:10.1186]
        Acta Endocrinologica SERUM LIPOPROTEIN-ASSOCIATED PHOSPHOLIPASE A2 IN MALES WITH METABOLIC SYNDROME AND OBSTRUCTIVE SLEEP APNEA. [jrnl:1841098]
        Biomarker for diagnosis of moyamoya disease [:]
        JOURNAL OF THE FORMOSAN MEDICAL ASSOCIATION Circulating fatty-acid binding-protein 4 levels predict CV events in patients after coronary interventions [Pubmed: 32859455]
        chinese neurosurgical journal Can admission lipoprotein-associated phospholipase A2 predict the symptomatic cerebral vasospasm following aneurysmal subarachnoid hemorrhage? []
        留言咨詢
        亚洲精品国产情侣AV在线,日韩精品一区二区三区色欲AV,免费看高清黄A级毛片