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      1. 抵抗素(RETN)檢測試劑盒(酶聯免疫吸附試驗法)

        ELISA Kit for Resistin (RETN)

        ADSF; FIZZ3; RETN1; RSTN; XCP1; Adipose tissue-specific secretory factor; C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein; Cysteine-rich secreted FIZZ3

        • 抵抗素(RETN)檢測試劑盒(酶聯免疫吸附試驗法) 產品包裝(模擬)
        • 抵抗素(RETN)檢測試劑盒(酶聯免疫吸附試驗法) 產品包裝(模擬)
        • 抵抗素(RETN)檢測試劑盒(酶聯免疫吸附試驗法) 實驗結果圖
        • SEA847Mu.jpg 標準曲線圖
        • Certificate 通過ISO 9001、ISO 13485質量體系認證

        特異性

        本試劑盒用于檢測抵抗素(RETN),經檢測與其它相似物質無明顯交叉反應。
        由于受到技術及樣本來源的限制,不可能完成對所有相關或相似物質交叉反應檢測,因此本試劑盒有可能與未經檢測的其它物質有交叉反應。

        回收率

        分別于定值血清及血漿樣本中加入一定量的抵抗素(RETN)(加標樣品),重復測定并計算其均值,回收率為測定值與理論值的比率。

        樣本 回收率范圍(%) 平均回收率(%)
        serum(n=5) 92-101 98
        EDTA plasma(n=5) 96-104 101
        heparin plasma(n=5) 79-102 98

        精密度

        精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
        批內差:取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續測定20 次,分別計算不同濃度樣本的平均值及SD值。
        批間差:選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定,每個樣本使用同一試劑盒重復測定8次,分別計算不同濃度樣本的平均值及SD值。
        批內差: CV<10%
        批間差: CV<12%

        線性

        在定值血清及血漿樣本內加入適量的抵抗素(RETN),并倍比稀釋成1:2,1:4,1:8,1:16的待測樣本,線性范圍即為稀釋后樣本中抵抗素(RETN)含量的測定值與理論值的比率。

        樣本 1:2 1:4 1:8 1:16
        serum(n=5) 95-102% 90-104% 78-98% 78-99%
        EDTA plasma(n=5) 93-101% 79-101% 83-97% 91-99%
        heparin plasma(n=5) 83-93% 94-101% 79-99% 87-102%

        穩定性

        經測定,試劑盒在有效期內按推薦溫度保存,其活性降低率小于5%。
        為減小外部因素對試劑盒破壞前后檢測值的影響,實驗室的環境條件需盡量保持一致,尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

        實驗流程

        1. 實驗前標準品、試劑及樣本的準備;
        2. 加樣(標準品及樣本)100µL,37°C孵育1小時;
        3. 吸棄,加檢測溶液A100µL,37°C孵育1小時;
        4. 洗板3次;
        5. 加檢測溶液B100µL,37°C孵育30分鐘;
        6. 洗板5次;
        7. 加TMB底物90µL,37°C孵育10-20分鐘;
        8. 加終止液50µL,立即450nm讀數。

        實驗原理

        將抵抗素(RETN)抗體包被于96孔微孔板中,制成固相載體,向微孔中分別加入標準品或標本,其中的抵抗素(RETN)與連接于固相載體上的抗體結合,然后加入生物素化的抵抗素(RETN)抗體,將未結合的生物素化抗體洗凈后,加入HRP標記的親和素,再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉化成藍色,并在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中的抵抗素(RETN)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值),計算樣品濃度。

        相關產品

        編號 適用物種:Mus musculus (Mouse,小鼠) 應用(僅供研究使用,不用于臨床診斷!)
        RPA847Mu01 抵抗素(RETN)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
        RPA847Mu02 抵抗素(RETN)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
        PAA847Mu01 抵抗素(RETN)多克隆抗體 WB; IHC; ICC; IP.
        PAA847Mu02 抵抗素(RETN)多克隆抗體 WB; IHC; ICC; IP.
        LAA847Mu81 抵抗素(RETN)多克隆抗體(異硫氰酸熒光素標記) WB; IHC; ICC; IF.
        LAA847Mu71 抵抗素(RETN)多克隆抗體(生物素標記) WB; IHC; ICC.
        MAA847Mu21 抵抗素(RETN)單克隆抗體 WB; IHC; ICC; IP.
        MAA847Mu29 抵抗素(RETN)單克隆抗體 WB; IHC; ICC; IP.
        MAA847Mu22 抵抗素(RETN)單克隆抗體 WB; IHC; ICC; IP.
        MAA847Mu23 抵抗素(RETN)單克隆抗體 WB; IHC; ICC; IP.
        MAA847Mu24 抵抗素(RETN)單克隆抗體 WB; IHC; ICC; IP.
        MAA847Mu25 抵抗素(RETN)單克隆抗體 WB; IHC; ICC; IP.
        MAA847Mu27 抵抗素(RETN)單克隆抗體 WB; IHC; ICC; IP.
        SEA847Mu 抵抗素(RETN)檢測試劑盒(酶聯免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
        SCA847Mu 抵抗素(RETN)檢測試劑盒(化學發光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
        LMA847Mu 抵抗素(RETN)單因子檢測試劑盒(流式熒光發光法) Magnetic Luminex Assay for Antigen Detection.
        KSA847Mu01 抵抗素(RETN)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) Main materials for "Do It (ELISA Kit) Yourself".

        參考文獻

        雜志 參考文獻
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        Evid Based Complement Alternat Med Tinospora crispa [PubMed: 25821506]
        Journal of Longitudinal changes in adipose tissue of dairy cows from late pregnancy to lactation. Part 1: The adipokines apelin and resistin and their relationship to receptors linked with lipolysis [PubMed: 26686707]
        Genome Genetic Variations in Visfatin and Resistin Genes in Egyptian Type 3 Diabetic Patients With and Without Complications [PubMed: 25120108]
        Acta Histochemica Favorable outcomes of hydroxychloroquine in insulin resistance may be accomplished by adjustment of the endothelial dysfunction as well as the skewed balance of adipokines [science:S0065128116301234]
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        Oncotarget FAM3A enhances adipogenesis of 3T3-L1 preadipocytes via activation of ATP-P2 receptor-Akt signaling pathway [pubmed:28515350]
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        Toxicology Biological effects of adipocytes in sulfur mustard induced toxicity [pubmed:29129815]
        Breast?Cancer?Research Bashar Zeidan1, Antigoni Manousopoulou2, Diana J. Garay-Baquero2, 3, Cory H. White3, 7, Samantha ET Larkin1, Kathleen N. Potter1, Theodoros I?… [Pubmed:29566726]
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        Journal of Functional Foods Mango leaf tea promotes hepatoprotective effects in obese rats [Doi: 10.1016/j.jff.2018.09.010]
        Food?Research?International Bacupari (Garcinia brasiliensis) extract modulates intestinal microbiota and reduces oxidative stress and inflammation in obese rats [Doi: 10.1016/j.foodres.2019.04.012]
        Journal of Functional Foods Extruded sorghum (Sorghum bicolor L.) improves gut microbiota, reduces inflammation, and oxidative stress in obese rats fed a high-fat diet []
        Nat Commun Antidiabetic and cardiovascular beneficial effects of a liver-localized mitochondrial uncoupler [Pubmed: 31092829]
        Nutrients Hypothalamic–Pituitary and Adipose Tissue Responses to the Effect of Resistin in Sheep: The Integration of Leptin and Resistin Signaling Involving a Suppressor of?… [Pubmed: 31514318]
        GYNECOLOGICAL ENDOCRINOLOGY Saliva resistin as a screening marker of gestational diabetes mellitus [Pubmed: 32804001]
        Chinese Journal of Natural Medicines Tao-Hong-Si-Wu Decoction promotes angiogenesis after cerebral ischaemia in rats via platelet microparticles [Pubmed: 32768169]
        INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Changes in Expression of the Genes for the Leptin Signaling in Hypothalamic-Pituitary Selected Areas and Endocrine Responses to Long-Term Manipulation in Body … [Pubmed: 32545900]
        Seasonal and Nutritional Fluctuations in the mRNA Levels of the Short Form of the Leptin Receptor (LRa) in the Hypothalamus and Anterior Pituitary in Resistin?… [34438908]
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        Skeletal Muscle Fatty Acid Metabolism is Diverted Towards the Synthesis of Signaling Intermediates Without Fatty Acid Uptake Contribution in Dry Cows with Obesity?… []
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        亚洲精品国产情侣AV在线,日韩精品一区二区三区色欲AV,免费看高清黄A级毛片