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      1. 視黃醇結合蛋白4(RBP4)檢測試劑盒(酶聯免疫吸附試驗法)

        ELISA Kit for Retinol Binding Protein 4 (RBP4)

        PRBP; RBP; Plasma retinol-binding protein; Retinol Binding Protein 4, Plasma

        • 視黃醇結合蛋白4(RBP4)檢測試劑盒(酶聯免疫吸附試驗法) 產品包裝(模擬)
        • 視黃醇結合蛋白4(RBP4)檢測試劑盒(酶聯免疫吸附試驗法) 產品包裝(模擬)
        • 視黃醇結合蛋白4(RBP4)檢測試劑盒(酶聯免疫吸附試驗法) 實驗結果圖
        • SEA929Mu.jpg 標準曲線圖
        • Certificate 通過ISO 9001、ISO 13485質量體系認證

        特異性

        本試劑盒用于檢測視黃醇結合蛋白4(RBP4),經檢測與其它相似物質無明顯交叉反應。
        由于受到技術及樣本來源的限制,不可能完成對所有相關或相似物質交叉反應檢測,因此本試劑盒有可能與未經檢測的其它物質有交叉反應。

        精密度

        精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
        批內差:取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續測定20 次,分別計算不同濃度樣本的平均值及SD值。
        批間差:選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定,每個樣本使用同一試劑盒重復測定8次,分別計算不同濃度樣本的平均值及SD值。
        批內差: CV<10%
        批間差: CV<12%

        穩定性

        經測定,試劑盒在有效期內按推薦溫度保存,其活性降低率小于5%。
        為減小外部因素對試劑盒破壞前后檢測值的影響,實驗室的環境條件需盡量保持一致,尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

        實驗流程

        1. 實驗前標準品、試劑及樣本的準備;
        2. 加樣(標準品及樣本)100µL,37°C孵育1小時;
        3. 吸棄,加檢測溶液A100µL,37°C孵育1小時;
        4. 洗板3次;
        5. 加檢測溶液B100µL,37°C孵育30分鐘;
        6. 洗板5次;
        7. 加TMB底物90µL,37°C孵育10-20分鐘;
        8. 加終止液50µL,立即450nm讀數。

        實驗原理

        將視黃醇結合蛋白4(RBP4)抗體包被于96孔微孔板中,制成固相載體,向微孔中分別加入標準品或標本,其中的視黃醇結合蛋白4(RBP4)與連接于固相載體上的抗體結合,然后加入生物素化的視黃醇結合蛋白4(RBP4)抗體,將未結合的生物素化抗體洗凈后,加入HRP標記的親和素,再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉化成藍色,并在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中的視黃醇結合蛋白4(RBP4)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值),計算樣品濃度。

        相關產品

        編號 適用物種:Mus musculus (Mouse,小鼠) 應用(僅供研究使用,不用于臨床診斷!)
        RPA929Mu01 視黃醇結合蛋白4(RBP4)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
        RPA929Mu02 視黃醇結合蛋白4(RBP4)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
        PAA929Mu02 視黃醇結合蛋白4(RBP4)多克隆抗體 WB; IHC; ICC; IP.
        PAA929Mu01 視黃醇結合蛋白4(RBP4)多克隆抗體 WB; IHC; ICC; IP.
        LAA929Mu81 視黃醇結合蛋白4(RBP4)多克隆抗體(異硫氰酸熒光素標記) WB; IHC; ICC; IF.
        LAA929Mu71 視黃醇結合蛋白4(RBP4)多克隆抗體(生物素標記) WB; IHC; ICC.
        SEA929Mu 視黃醇結合蛋白4(RBP4)檢測試劑盒(酶聯免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
        LMA929Mu 視黃醇結合蛋白4(RBP4)單因子檢測試劑盒(流式熒光發光法) Magnetic Luminex Assay for Antigen Detection.
        KSA929Mu01 視黃醇結合蛋白4(RBP4)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) Main materials for "Do It (ELISA Kit) Yourself".

        參考文獻

        雜志 參考文獻
        Diabetes Technology & Therapeutics Proteomic Identification of Human Urinary Biomarkers in Diabetes Mellitus Type 2 [Liebert: 20100078]
        Journal of Pharmaceutical and Biomedical Analysis Effect of high dose thiamine on the levels of urinary protein biomarkers in diabetes mellitus type 2 [PubMed: 21130593]
        Evid Based Complement Alternat Med Quercetin Protects against Cadmium-Induced Renal Uric Acid Transport System Alteration and Lipid Metabolism Disorder in Rats [PubMed: PMC3368504]
        Journal of Endocrinology Combined galanin with insulin improves insulin sensitivity of diabetic rat muscles [Pubmed:24501381]
        J Endocrinol Central alarin ameliorated insulin resistance of adipocytes in type 2 diabetic rats [Pubmed:25240061]
        Peptides Effect of endogenous galanin on glucose transporter 4 expression in cardiac muscle of type 2 diabetic rats [Pubmed:25445608]
        Obesity Surgery Long-term Effect of Ileal Transposition on Adipokine Serum Level in Zucker (Orl)-Lepr fa Fatty Rats [Pubmed:25697126]
        Clin Exp Nephrol Differentially expressed urinary biomarkers in children with idiopathic nephrotic syndrome [PubMed: 26351173]
        Experimental and Therapeutic Medicine Expression of retinol binding protein 4 and nuclear factor-??B in diabetic rats with atherosclerosis and the intervention effect of pioglitazone [Pubmed:27446311]
        Experimental and Therapeutic Medicine Expression?of?retinol?binding?protein?4?and?nuclear?factor-κB in?diabetic?rats?with?atherosclerosisand the?intervention?effect?of?pioglitazone. [pubmed:27446311]
        Journal of Chromatography A Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosis [S0021967317311858]
        BMC Cancer Screening for immune-potentiating antigens from hepatocellular carcinoma patients after radiofrequency ablation by serum proteomic analysis [Pubmed:29386009]
        Nutrition &?Diabetes Lack of pronounced changes in the expression of fatty acid handling proteins in adipose tissue and plasma of morbidly obese humans [Pubmed:29335416]
        Molecular?Medicine?Reports RBP4 regulates trophoblastic cell proliferation and invasion via the PI3K/AKT signaling pathway [Pubmed:30015949]
        Relationship between retinol and risk of diabetic retinopathy: a case-control study []
        Scientific Reports Retinol binding protein 4 abundance in plasma and tissues is related to body fat deposition in cattle [Pubmed: 31147589]
        Potassium supplementation blunts the effects of high salt intake on serum retinol‐binding protein 4 levels in healthy individuals []
        Docosahexaenoic acid (DHA), a nutritional supplement, modulates steroid insensitivity in asthma []
        Eur J Nutr The dietary inflammatory index is associated with anti-and pro-inflammatory adipokines in Brazilian schoolchildren [33575861]
        Eur J Pediatr Pro-and anti-inflammatory adipokines are associated with cardiometabolic risk markers in Brazilian schoolchildren [33834274]
        Sci Rep Ileal transposition helps to regulate plasma hepatokine levels in obese Zucker (Crl: ZUC (ORL)-Lepr fa) rats [33833309]
        Int Arch Occup Environ Health Biomarkers of cadmium exposure and renal function in estuarine adult villagers [34773507]
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