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      1. 轉化生長因子β1(TGFb1)檢測試劑盒(酶聯免疫吸附試驗法)

        ELISA Kit for Transforming Growth Factor Beta 1 (TGFb1)

        TGF-B1; CED; DPD1; LAP; Camurati-Engelmann Disease; Latency-associated peptide

        • 轉化生長因子β1(TGFb1)檢測試劑盒(酶聯免疫吸附試驗法) 產品包裝(模擬)
        • 轉化生長因子β1(TGFb1)檢測試劑盒(酶聯免疫吸附試驗法) 產品包裝(模擬)
        • 轉化生長因子β1(TGFb1)檢測試劑盒(酶聯免疫吸附試驗法) 實驗結果圖
        • SEA124Mu.jpg 標準曲線圖
        • Certificate 通過ISO 9001、ISO 13485質量體系認證

        特異性

        本試劑盒用于檢測轉化生長因子β1(TGFb1),經檢測與其它相似物質無明顯交叉反應。
        由于受到技術及樣本來源的限制,不可能完成對所有相關或相似物質交叉反應檢測,因此本試劑盒有可能與未經檢測的其它物質有交叉反應。

        回收率

        分別于定值血清及血漿樣本中加入一定量的轉化生長因子β1(TGFb1)(加標樣品),重復測定并計算其均值,回收率為測定值與理論值的比率。

        樣本 回收率范圍(%) 平均回收率(%)
        serum(n=5) 82-105 98
        EDTA plasma(n=5) 98-105 101
        heparin plasma(n=5) 92-99 95

        精密度

        精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
        批內差:取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續測定20 次,分別計算不同濃度樣本的平均值及SD值。
        批間差:選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定,每個樣本使用同一試劑盒重復測定8次,分別計算不同濃度樣本的平均值及SD值。
        批內差: CV<10%
        批間差: CV<12%

        線性

        在定值血清及血漿樣本內加入適量的轉化生長因子β1(TGFb1),并倍比稀釋成1:2,1:4,1:8,1:16的待測樣本,線性范圍即為稀釋后樣本中轉化生長因子β1(TGFb1)含量的測定值與理論值的比率。

        樣本 1:2 1:4 1:8 1:16
        serum(n=5) 90-97% 92-99% 84-98% 92-101%
        EDTA plasma(n=5) 95-102% 99-105% 91-99% 96-104%
        heparin plasma(n=5) 82-90% 98-105% 91-99% 99-105%

        穩定性

        經測定,試劑盒在有效期內按推薦溫度保存,其活性降低率小于5%。
        為減小外部因素對試劑盒破壞前后檢測值的影響,實驗室的環境條件需盡量保持一致,尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

        實驗流程

        1. 實驗前標準品、試劑及樣本的準備;
        2. 加樣(標準品及樣本)100µL,37°C孵育1小時;
        3. 吸棄,加檢測溶液A100µL,37°C孵育1小時;
        4. 洗板3次;
        5. 加檢測溶液B100µL,37°C孵育30分鐘;
        6. 洗板5次;
        7. 加TMB底物90µL,37°C孵育10-20分鐘;
        8. 加終止液50µL,立即450nm讀數。

        實驗原理

        將轉化生長因子β1(TGFb1)抗體包被于96孔微孔板中,制成固相載體,向微孔中分別加入標準品或標本,其中的轉化生長因子β1(TGFb1)與連接于固相載體上的抗體結合,然后加入生物素化的轉化生長因子β1(TGFb1)抗體,將未結合的生物素化抗體洗凈后,加入HRP標記的親和素,再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉化成藍色,并在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中的轉化生長因子β1(TGFb1)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值),計算樣品濃度。

        相關產品

        編號 適用物種:Mus musculus (Mouse,小鼠) 應用(僅供研究使用,不用于臨床診斷!)
        RPA124Mu02 轉化生長因子β1(TGFb1)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
        APA124Mu01 轉化生長因子β1(TGFb1)活性蛋白 Cell?culture;?Activity?Assays.
        RPA124Mu01 轉化生長因子β1(TGFb1)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
        EPA124Mu61 轉化生長因子β1(TGFb1)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
        PAA124Mu01 轉化生長因子β1(TGFb1)多克隆抗體 WB; IHC; ICC; IP.
        PAA124Mu02 轉化生長因子β1(TGFb1)多克隆抗體 WB; IHC; ICC; IP.
        LAA124Mu81 轉化生長因子β1(TGFb1)多克隆抗體(異硫氰酸熒光素標記) WB; IHC; ICC; IF.
        LAA124Mu71 轉化生長因子β1(TGFb1)多克隆抗體(生物素標記) WB; IHC; ICC.
        SEA124Mu 轉化生長因子β1(TGFb1)檢測試劑盒(酶聯免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
        MEA124Mu 轉化生長因子β1(TGFb1)檢測試劑盒(酶聯免疫吸附試驗法,小樣本) Enzyme-linked immunosorbent assay for Antigen Detection.
        SCA124Mu 轉化生長因子β1(TGFb1)檢測試劑盒(化學發光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
        LMA124Mu 轉化生長因子β1(TGFb1)單因子檢測試劑盒(流式熒光發光法) Magnetic Luminex Assay for Antigen Detection.
        KSA124Mu01 轉化生長因子β1(TGFb1)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) Main materials for "Do It (ELISA Kit) Yourself".

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        Immunomodulatory effects of sCD83 via induction of TGF-β1 and IDO: an in vitro and rat orthotopic liver transplantation study []
        BMC Complement Med Ther Suppression of autophagy through JAK2/STAT3 contributes to the therapeutic action of rhynchophylline on asthma [33413331]
        Life Sci Celecoxib ameliorates liver cirrhosis via reducing inflammation and oxidative stress along spleen-liver axis in rats [33577848]
        CLINICAL SCIENCE Berberine accelerated wound healing by restoring TrxR1/JNK in diabetes [33491733]
        Bioact Mater New design to remove leukocytes from platelet-rich plasma (PRP) based on cell dimension rather than density [33842739]
        Acta Biomater Highly interconnected inverse opal extracellular matrix scaffolds enhance stem cell therapy in limb ischemia [33878473]
        Mol Cell Biochem Human umbilical cord-derived mesenchymal stem cells and their chondroprogenitor derivatives reduced pain and inflammation signaling and promote regeneration in??- [33864569]
        Cell Death & Disease Sodium/glucose cotransporter 1-dependent metabolic alterations induce tamoxifen resistance in breast cancer by promoting macrophage M2 polarization [34006822]
        PLoS One Beneficial impact of cathelicidin on hypersensitivity pneumonitis treatment?aIn vivo studies [33999928]
        Vascular pharmacology Nectin-4 promotes lymphangiogenesis and lymphatic metastasis in breast cancer by regulating CXCR4-LYVE1 axis [33945869]
        Equine Vet J Limited added value of negative pressure wound therapy compared to calcium alginate dressings for second intention healing in a non?\contaminated and??- [34115409]
        Stem Cells International The Paracrine Effect of Adipose-Derived Stem Cells Orchestrates Competition between Different Damaged Dermal Fibroblasts to Repair UVB-Induced Skin??- [33381190]
        Journal of Pharmacy and Pharmacology Pharmacodynamic evaluation of dihydroxyflavone derivate chrysin in a guinea pig model of allergic asthma [33793800]
        Bull. Egypt. Soc. Physiol. Sci. Flaxseed Oil Supplementation Afford Comparable Therapeutic effect to Metformin in Bleomycin Induced Pulmonary Fibrosis Rat model []
        D£?ˉ Role of Angiotensin II and Renal Nerves on TGF-b/SmadPathway in Diabetic Nephropathy Rat Model []
        Bioactive Materials Calcium phosphate-based materials regulate osteoclast-mediated osseointegration []
        Mediators of Inflammation Sparstolonin B Exerts Therapeutic Effects on Collagen-Induced Arthritis by Inhibiting the NLRP3 Inflammasome and Reducing the Activity of |á1, 3 ?- []
        Manuscript title Microparticles Containing MiR-625-5p In Coronary Artery Reduce Myocardial Fibrosis After Myocardial Infarction []
        Andrology X‐ray micro‐computed tomography in the assessment of penile cavernous fibrosis in a rabbit castration model [34236146]
        Aging Yiqi Jiemin decoction alleviates allergic rhinitis in a guinea pig model by suppressing inflammation, restoring Th1/Th2 balance, and improving cellular?… [34315133]
        Tissue Eng Regen Med Effect of Leukocyte-Platelet Rich Fibrin (L-PRF) on Tissue Regeneration and Proliferation of Human Gingival Fibroblast Cells Cultured Using a Modified Method [34339025]
        Am J Transl Res Wuling San and Xiao Chaihu Decoction affect airway inflammatory response and airway smooth muscle cell proliferation in mice with allergic asthma via miR?… [34786062]
        Biomacromolecules In Situ Forming Cellulose Nanofibril-Reinforced Hyaluronic Acid Hydrogel for Cartilage Regeneration [34723499]
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        Biomedicines Exploring Epithelial–Mesenchymal Transition Signals in Endometriosis Diagnosis and In Vitro Fertilization Outcomes [34829910]
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        Cancer Commun (Lond) Anti‐PD‐L1/TGF‐βR fusion protein (SHR‐1701) overcomes disrupted lymphocyte recovery‐induced resistance to PD‐1/PD‐L1 inhibitors in lung cancer [Pubmed:34981670]
        Polymers (Basel) 3D Printed Scaffold Based on Type I Collagen/PLGA_TGF-β1 Nanoparticles Mimicking the Growth Factor Footprint of Human Bone Tissue [Pubmed:35267680]
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        Antioxidants Bio-Evaluation of the Wound Healing Activity of Artemisia judaica L. as Part of the Plant's Use in Traditional Medicine; Phytochemical, Antioxidant, Anti-Inflammatory?… [Pubmed:35204215]
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