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      1. 血管內皮生長因子A(VEGFA)檢測試劑盒(酶聯免疫吸附試驗法)

        ELISA Kit for Vascular Endothelial Growth Factor A (VEGFA)

        VEGF; VEGF-A; VPF; Vascular Endothelial Cell Growth Factor A; Vascular Permeability Factor

        • 血管內皮生長因子A(VEGFA)檢測試劑盒(酶聯免疫吸附試驗法) 產品包裝(模擬)
        • 血管內皮生長因子A(VEGFA)檢測試劑盒(酶聯免疫吸附試驗法) 產品包裝(模擬)
        • 血管內皮生長因子A(VEGFA)檢測試劑盒(酶聯免疫吸附試驗法) 實驗結果圖
        • SEA143Mu.jpg 標準曲線圖
        • Certificate 通過ISO 9001、ISO 13485質量體系認證

        特異性

        本試劑盒用于檢測血管內皮生長因子A(VEGFA),經檢測與其它相似物質無明顯交叉反應。
        由于受到技術及樣本來源的限制,不可能完成對所有相關或相似物質交叉反應檢測,因此本試劑盒有可能與未經檢測的其它物質有交叉反應。

        回收率

        分別于定值血清及血漿樣本中加入一定量的血管內皮生長因子A(VEGFA)(加標樣品),重復測定并計算其均值,回收率為測定值與理論值的比率。

        樣本 回收率范圍(%) 平均回收率(%)
        serum(n=5) 97-105 102
        EDTA plasma(n=5) 86-101 93
        heparin plasma(n=5) 96-103 101

        精密度

        精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
        批內差:取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續測定20 次,分別計算不同濃度樣本的平均值及SD值。
        批間差:選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定,每個樣本使用同一試劑盒重復測定8次,分別計算不同濃度樣本的平均值及SD值。
        批內差: CV<10%
        批間差: CV<12%

        線性

        在定值血清及血漿樣本內加入適量的血管內皮生長因子A(VEGFA),并倍比稀釋成1:2,1:4,1:8,1:16的待測樣本,線性范圍即為稀釋后樣本中血管內皮生長因子A(VEGFA)含量的測定值與理論值的比率。

        樣本 1:2 1:4 1:8 1:16
        serum(n=5) 83-98% 89-96% 79-91% 95-104%
        EDTA plasma(n=5) 90-104% 80-99% 96-103% 85-99%
        heparin plasma(n=5) 86-98% 86-101% 85-101% 80-95%

        穩定性

        經測定,試劑盒在有效期內按推薦溫度保存,其活性降低率小于5%。
        為減小外部因素對試劑盒破壞前后檢測值的影響,實驗室的環境條件需盡量保持一致,尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

        實驗流程

        1. 實驗前標準品、試劑及樣本的準備;
        2. 加樣(標準品及樣本)100µL,37°C孵育1小時;
        3. 吸棄,加檢測溶液A100µL,37°C孵育1小時;
        4. 洗板3次;
        5. 加檢測溶液B100µL,37°C孵育30分鐘;
        6. 洗板5次;
        7. 加TMB底物90µL,37°C孵育10-20分鐘;
        8. 加終止液50µL,立即450nm讀數。

        實驗原理

        將血管內皮生長因子A(VEGFA)抗體包被于96孔微孔板中,制成固相載體,向微孔中分別加入標準品或標本,其中的血管內皮生長因子A(VEGFA)與連接于固相載體上的抗體結合,然后加入生物素化的血管內皮生長因子A(VEGFA)抗體,將未結合的生物素化抗體洗凈后,加入HRP標記的親和素,再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉化成藍色,并在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中的血管內皮生長因子A(VEGFA)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值),計算樣品濃度。

        相關產品

        編號 適用物種:Mus musculus (Mouse,小鼠) 應用(僅供研究使用,不用于臨床診斷!)
        RPA143Mu01 血管內皮生長因子A(VEGFA)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
        APA143Mu01 血管內皮生長因子A(VEGFA)活性蛋白 Cell?culture;?Activity?Assays.
        PAA143Mu01 血管內皮生長因子A(VEGFA)多克隆抗體 WB; IHC; ICC; IP.
        LAA143Mu81 血管內皮生長因子A(VEGFA)多克隆抗體(異硫氰酸熒光素標記) WB; IHC; ICC; IF.
        LAA143Mu71 血管內皮生長因子A(VEGFA)多克隆抗體(生物素標記) WB; IHC; ICC.
        SEA143Mu 血管內皮生長因子A(VEGFA)檢測試劑盒(酶聯免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
        MEA143Mu 血管內皮生長因子A(VEGFA)檢測試劑盒(酶聯免疫吸附試驗法,小樣本) Enzyme-linked immunosorbent assay for Antigen Detection.
        SCA143Mu 血管內皮生長因子A(VEGFA)檢測試劑盒(化學發光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
        LMA143Mu 血管內皮生長因子A(VEGFA)單因子檢測試劑盒(流式熒光發光法) Magnetic Luminex Assay for Antigen Detection.

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