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      1. 軸突生長誘向因子1(Ntn1)檢測試劑盒(酶聯免疫吸附試驗法)

        ELISA Kit for Netrin 1 (Ntn1)

        NTN1L; Epididymis tissue protein Li 131P

        • 軸突生長誘向因子1(Ntn1)檢測試劑盒(酶聯免疫吸附試驗法) 產品包裝(模擬)
        • 軸突生長誘向因子1(Ntn1)檢測試劑盒(酶聯免疫吸附試驗法) 產品包裝(模擬)
        • 軸突生長誘向因子1(Ntn1)檢測試劑盒(酶聯免疫吸附試驗法) 實驗結果圖
        • SEB827Ga.jpg 標準曲線圖
        • Certificate 通過ISO 9001、ISO 13485質量體系認證

        特異性

        本試劑盒用于檢測軸突生長誘向因子1(Ntn1),經檢測與其它相似物質無明顯交叉反應。
        由于受到技術及樣本來源的限制,不可能完成對所有相關或相似物質交叉反應檢測,因此本試劑盒有可能與未經檢測的其它物質有交叉反應。

        回收率

        分別于定值血清及血漿樣本中加入一定量的軸突生長誘向因子1(Ntn1)(加標樣品),重復測定并計算其均值,回收率為測定值與理論值的比率。

        樣本 回收率范圍(%) 平均回收率(%)
        serum(n=5) 80-94 83
        EDTA plasma(n=5) 91-102 97
        heparin plasma(n=5) 81-99 87

        精密度

        精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
        批內差:取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續測定20 次,分別計算不同濃度樣本的平均值及SD值。
        批間差:選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定,每個樣本使用同一試劑盒重復測定8次,分別計算不同濃度樣本的平均值及SD值。
        批內差: CV<10%
        批間差: CV<12%

        線性

        在定值血清及血漿樣本內加入適量的軸突生長誘向因子1(Ntn1),并倍比稀釋成1:2,1:4,1:8,1:16的待測樣本,線性范圍即為稀釋后樣本中軸突生長誘向因子1(Ntn1)含量的測定值與理論值的比率。

        樣本 1:2 1:4 1:8 1:16
        serum(n=5) 89-105% 99-105% 96-105% 98-105%
        EDTA plasma(n=5) 93-101% 88-105% 96-105% 78-92%
        heparin plasma(n=5) 92-104% 89-97% 79-101% 88-104%

        穩定性

        經測定,試劑盒在有效期內按推薦溫度保存,其活性降低率小于5%。
        為減小外部因素對試劑盒破壞前后檢測值的影響,實驗室的環境條件需盡量保持一致,尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

        實驗流程

        1. 實驗前標準品、試劑及樣本的準備;
        2. 加樣(標準品及樣本)100µL,37°C孵育1小時;
        3. 吸棄,加檢測溶液A100µL,37°C孵育1小時;
        4. 洗板3次;
        5. 加檢測溶液B100µL,37°C孵育30分鐘;
        6. 洗板5次;
        7. 加TMB底物90µL,37°C孵育10-20分鐘;
        8. 加終止液50µL,立即450nm讀數。

        實驗原理

        將軸突生長誘向因子1(Ntn1)抗體包被于96孔微孔板中,制成固相載體,向微孔中分別加入標準品或標本,其中的軸突生長誘向因子1(Ntn1)與連接于固相載體上的抗體結合,然后加入生物素化的軸突生長誘向因子1(Ntn1)抗體,將未結合的生物素化抗體洗凈后,加入HRP標記的親和素,再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉化成藍色,并在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中的軸突生長誘向因子1(Ntn1)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值),計算樣品濃度。

        相關產品

        編號 適用物種:Chicken (Gallus,雞) 應用(僅供研究使用,不用于臨床診斷!)
        RPB827Ga01 軸突生長誘向因子1(Ntn1)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
        PAB827Ga01 軸突生長誘向因子1(Ntn1)多克隆抗體 WB; IHC; ICC; IP.
        MAB827Ga21 軸突生長誘向因子1(Ntn1)單克隆抗體 WB; IHC; ICC; IP.
        SEB827Ga 軸突生長誘向因子1(Ntn1)檢測試劑盒(酶聯免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
        LMB827Ga 軸突生長誘向因子1(Ntn1)單因子檢測試劑盒(流式熒光發光法) Magnetic Luminex Assay for Antigen Detection.

        參考文獻

        雜志 參考文獻
        Transplantation Proceedings Netrin-1: A Novel Universal Biomarker of Human Kidney Injury [PubMed: 20620466]
        Biomarkers Plasma netrin-1 is a diagnostic biomarker of human cancers [PubMed: PMC3143477]
        Journal of Maternal-Fetal and Neonatal Medicine Cord blood netrin-1 and -4 concentrations in term pregnancies with normal, restricted and increased fetal growth [Informahealthcare: Source]
        4 Netrin-1 promotes adipose tissue macrophage retention and insulin resistance in obesity [Pubmed:24584118]
        J Matern?Fetal?Neonatal Med.? Cord blood netrin-1 and-4 concentrations in term pregnancies with normal, restricted and increased fetal growth [Pubmed:24716747]
        Renal failure. Urinary netrin-1 and KIM-1 as early biomarkers for septic acute kidney injury [Pubmed:25154466]
        Stem Cell Research & Therapy Netrin-1 acts as a non-canonical angiogenic factor produced by human Wharton’s jelly mesenchymal stem cells (WJ-MSC) [articles:10.1186]
        Oncotarget The effect of aspirin on circulating netrin-1 levels in humans is dependent on the inflammatory status of the vascular endothelium. [pubmed:29156815]
        Frontiers in?Cellular?Neuroscience Neuroprotection Exerted by Netrin-1 and Kinesin Motor KIF1A in Secondary Brain Injury following Experimental Intracerebral Hemorrhage in Rats [Pubmed:29375318]
        Scientific Reports Netrin-1 promotes diabetic corneal wound healing through molecular mechanisms mediated via the adenosine 2B receptor [Pubmed:29662125]
        Family Practice and Palliative Care Relationship of serum netrin-1 levels with breast masses [Doi: 10.22391/fppc.467700]
        JOURNAL OF BIOLOGICAL CHEMISTRY Role of microRNA in CB1 antagonist-mediated regulation of adipose tissue macrophage polarization and chemotaxis during diet-induced obesity [Pubmed: 30910812]
        International Journal of Molecular?Sciences Downregulation of the Netrin-1 Receptor UNC5b Underlies Increased Placental Angiogenesis in Human Gestational Diabetes Mellitus [Pubmed: 30897795]
        Cancer Biomark Serum netrin-1 as a biomarker for colorectal cancer detection [Pubmed: 32474463]
        Níveis Elevados de Netrina-1 e IL-1β em Mulheres Idosas com SCA: Pior Prognóstico no Acompanhamento de Dois Anos []
        Tuberk Toraks Association of plasma netrin-1 level with inflammatory balance and comorbidities in patients with acute exacerbation of COPD [33853303]
        Adv Healthc Mater Conductive Hydrogel Conduits with Growth Factor Gradients for Peripheral Nerve Repair in Diabetics with Non‐suture Tape [Pubmed:35670309]
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