亚洲精品国产情侣AV在线,日韩精品一区二区三区色欲AV,免费看高清黄A级毛片

  • <object id="jwsct"><legend id="jwsct"></legend></object>
    <object id="jwsct"></object>
  • <s id="jwsct"></s>
      1. 白蛋白(ALB)檢測試劑盒(酶聯免疫吸附試驗法)

        ELISA Kit for Albumin (ALB)

        Serum albumin

        • 白蛋白(ALB)檢測試劑盒(酶聯免疫吸附試驗法) 產品包裝(模擬)
        • 白蛋白(ALB)檢測試劑盒(酶聯免疫吸附試驗法) 產品包裝(模擬)
        • 白蛋白(ALB)檢測試劑盒(酶聯免疫吸附試驗法) 實驗結果圖
        • CEB028Rb.jpg 標準曲線圖
        • Certificate 通過ISO 9001、ISO 13485質量體系認證

        特異性

        本試劑盒用于檢測白蛋白(ALB),經檢測與其它相似物質無明顯交叉反應。
        由于受到技術及樣本來源的限制,不可能完成對所有相關或相似物質交叉反應檢測,因此本試劑盒有可能與未經檢測的其它物質有交叉反應。

        回收率

        分別于定值血清及血漿樣本中加入一定量的白蛋白(ALB)(加標樣品),重復測定并計算其均值,回收率為測定值與理論值的比率。

        樣本 回收率范圍(%) 平均回收率(%)
        serum(n=5) 88-103 99
        EDTA plasma(n=5) 82-101 97
        heparin plasma(n=5) 88-96 93

        精密度

        精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
        批內差:取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續測定20 次,分別計算不同濃度樣本的平均值及SD值。
        批間差:選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定,每個樣本使用同一試劑盒重復測定8次,分別計算不同濃度樣本的平均值及SD值。
        批內差: CV<10%
        批間差: CV<12%

        線性

        在定值血清及血漿樣本內加入適量的白蛋白(ALB),并倍比稀釋成1:2,1:4,1:8,1:16的待測樣本,線性范圍即為稀釋后樣本中白蛋白(ALB)含量的測定值與理論值的比率。

        樣本 1:2 1:4 1:8 1:16
        serum(n=5) 96-105% 79-103% 91-105% 85-101%
        EDTA plasma(n=5) 98-105% 78-102% 95-104% 78-103%
        heparin plasma(n=5) 79-94% 78-95% 86-96% 94-103%

        穩定性

        經測定,試劑盒在有效期內按推薦溫度保存,其活性降低率小于5%。
        為減小外部因素對試劑盒破壞前后檢測值的影響,實驗室的環境條件需盡量保持一致,尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

        實驗流程

        1. 實驗前標準品、試劑及樣本的準備;
        2. 加樣(標準品及樣本)50µL,
            加入50µL檢測液A(臨用前配制);
            37°C溫育1小時。
        3. 洗板3次;
        4. 加檢測溶液B100µL,37°C孵育30分鐘;
        5. 洗板5次;
        6. 加TMB底物90µL,37°C孵育10-20分鐘;
        7. 加終止液50µL,立即450nm讀數。

        實驗原理

        本試劑盒應用競爭抑制酶聯免疫分析法測定標本中待測物質水平。將白蛋白(ALB)單克隆抗體包被微孔板,制成固相載體,往包被抗體的微孔中同時加入生物素標記的抗原和待測抗原(標準品或樣本),待測抗原與生物素標記抗原對特異性抗體進行競爭結合。溫育后經洗滌去掉未結合物,然后加入HRP標記的親和素,經過溫育和徹底洗滌后加入底物TMB顯色。TMB在過氧化物酶的催化下轉化成藍色,并在酸的作用下轉化成最終的黃色。待測標本濃度越高,標記抗原和抗體的結合就越受到抑制,顯色愈淺。顯色的深淺與酶量呈正相關,而與樣品中待測物質含量呈負相關。用酶標儀在450nm波長下測定吸光度(O.D.值),計算樣品濃度。

        相關產品

        編號 適用物種:Oryctolagus cuniculus (Rabbit,兔) 應用(僅供研究使用,不用于臨床診斷!)
        NPB028Rb01 白蛋白(ALB)天然蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
        RPB028Rb01 白蛋白(ALB)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
        RPB028Rb02 白蛋白(ALB)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
        PAB028Rb52 白蛋白(ALB)多克隆抗體 WB; IHC; ICC; IP.
        PAB028Rb01 白蛋白(ALB)多克隆抗體 WB; IHC; ICC; IP.
        PAB028Rb51 白蛋白(ALB)多克隆抗體 WB; IHC; ICC; IP.
        LAB028Rb81 白蛋白(ALB)多克隆抗體(異硫氰酸熒光素標記) WB; IHC; ICC; IF.
        LAB028Rb71 白蛋白(ALB)多克隆抗體(生物素標記) WB; IHC; ICC.
        CEB028Rb 白蛋白(ALB)檢測試劑盒(酶聯免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
        LMB028Rb 白蛋白(ALB)單因子檢測試劑盒(流式熒光發光法) Magnetic Luminex Assay for Antigen Detection.
        KSB028Rb11 白蛋白(ALB)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) Main materials for "Do It (ELISA Kit) Yourself".

        參考文獻

        雜志 參考文獻
        PLoS One Simvastatin Reduces Endotoxin-Induced Acute Lung Injury by Decreasing Neutrophil Recruitment and Radical Formation [PubMed: 22701728]
        Liver International Albumin administration protects against bilirubin-induced auditory brainstem dysfunction in Gunn rat pups [Onlinelibrary: liv.12219]
        Journal of Infectious Diseases Adjunctive corticosteroid therapy improves lung immunopathology and survival during severe secondary pneumococcal pneumonia in mice [Pubmed: 24273183]
        Toxins (Basel). Increased Circulating Levels of Vitamin D Binding Protein in MS Patients [Pubmed:25590278]
        Mol Cell Biochem The wnt/β-catenin signaling pathway participates in rhein ameliorating kidney injury in DN mice [PubMed: 26346164]
        PLoS?One Collecting Protein Biomarkers in Breath Using Electret Filters: A Preliminary Method on New Technical Model and Human Study [Pubmed:26934615]
        Journal of Biomaterials and Tissue Engineering 3D Printing of Differentiated Bone Marrow Mesenchymal Cells as a New Method for Liver Tissue Engineering [art00007]
        Experimental and molecular pathology Alteration of Connexin43 expression in a rat model of obesity-related glomerulopathy. [pubmed:29246788]
        Inflammation Resolvin D1 Promotes SIRT1 Expression to Counteract the Activation of STAT3 and NF-κB in Mice with Septic-Associated Lung Injury [Pubmed:30014231]
        Journal of Developmental Origins of?Health?and?Disease Maternal low-quality protein diet exerts sex-specific effects on plasma amino acid profile and alters hepatic expression of methyltransferases in adult rat offspring [Pubmed:29582727]
        Medicine Electret filter collects more exhaled albumin than glass condenser: A method comparison based on human study [Pubmed:29384875]
        Stem?Cell?Research?&?Therapy Therapeutic efficiency of human amniotic epithelial stem cell-derived functional hepatocyte-like cells in mice with acute hepatic failure [Pubmed: 30463600]
        JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A Decellularized Human Liver Scaffold‐Based Three‐dimensional Culture System Facilitate HBV Infection [Pubmed: 30963688]
        biomedicine & pharmacotherapy Treatment with 3, 4-dihydroxyphenylethyl alcohol glycoside ameliorates sepsis-induced ALI in mice by reducing inflammation and regulating M1 polarization [Pubmed: 31146107]
        biochemical and biophysical research communications Anti-proteinuria effect of antibody against ANGPTL3 coil-coiled domain on adriamycin-induced nephropathy in mice [Pubmed: 31256934]
        Diabetes, Metabolic Syndrome and Obesity A small molecule inhibitor MCC950 ameliorates kidney injury in diabetic nephropathy by inhibiting NLRP3 inflammasome activation [Pubmed: 31447572]
        Molecular and Cellular Endocrinology Diiodothyronines regulate metabolic homeostasis in primary human hepatocytes by modulating mTORC1 and mTORC2 activity [Pubmed: 31580898]
        BMC Veterinary Research Preliminary study on the effects of enrofloxacin, flunixin meglumine and pegbovigrastim on Mycoplasma bovis pneumonia [Pubmed: 31655595]
        International Immunopharmacology Ghrelin alleviates traumatic brain injury-induced acute lung injury through pyroptosis/NF-κB pathway [Pubmed: 31918060]
        HYPERTENSION RESEARCH Sodium butyrate ameliorates deoxycorticosterone acetate/salt-induced hypertension and renal damage by inhibiting the MR/SGK1 pathway [Pubmed: 32908237]
        Obesity Surgery Sleeve Gastrectomy Attenuates Diabetic Nephropathy by Upregulating Nephrin Expressions in Diabetic Obese Rats [Pubmed: 32399849]
        Protective Role of Training and Selenium Consumption Against Renal Toxicity Induced by Cadmium in Rats []
        Nutrition Gut Dysbiosis Contribute to High Fructose Induced Salt Sensitive Hypertension In Sprague-Dawley Rats []
        sensors and actuators b-chemical A light-induced self-powered competitive immunosensor for the detection of platelet derived growth factor-BB via an elaborately assembled bioconjugate []
        Biochem Biophys Res Commun cGAS promotes sepsis in radiotherapy of cancer by up-regulating caspase-11 signaling [33721834]
        Journal of Chinese Pharmaceutical Sciences A simple and sensitive electrochemical impedimetric immunosensor for the detection of apolipoprotein E4 based on CeZnO nanoparticle []
        Inflammation Syringaresinol Resisted Sepsis-Induced Acute Lung Injury by Suppressing Pyroptosis Via the Oestrogen Receptor-β Signalling Pathway [34807349]
        Nat Commun GP73 is a TBC-domain Rab GTPase-activating protein contributing to the pathogenesis of non-alcoholic fatty liver disease without obesity [34853313]
        ACS Appl Mater Interfaces Emitter–Quencher Pair of Single Atomic Co Sites and Monolayer Titanium Carbide MXenes for Luminol Chemiluminescent Reactions [34914377]
        Food & Function Moderation of gut microbiota and bile acid metabolism by chlorogenic acid improves high-fructose-induced salt-sensitive hypertension in mice [Pubmed:35708145]
        Authorea Syringaresinol attenuates kidney injury by inhibition of pyroptosis via activating the Nrf2 antioxidant pathway in diabetic nephropathy []
        留言咨詢
        亚洲精品国产情侣AV在线,日韩精品一区二区三区色欲AV,免费看高清黄A级毛片