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      1. 表皮生長因子(EGF)檢測試劑盒(酶聯免疫吸附試驗法)

        ELISA Kit for Epidermal Growth Factor (EGF)

        URG; Beta-Urogastrone; Pro-epidermal growth factor

        • 表皮生長因子(EGF)檢測試劑盒(酶聯免疫吸附試驗法) 產品包裝(模擬)
        • 表皮生長因子(EGF)檢測試劑盒(酶聯免疫吸附試驗法) 產品包裝(模擬)
        • 表皮生長因子(EGF)檢測試劑盒(酶聯免疫吸附試驗法) 實驗結果圖
        • SEA560Ra.jpg 標準曲線圖
        • Certificate 通過ISO 9001、ISO 13485質量體系認證

        特異性

        本試劑盒用于檢測表皮生長因子(EGF),經檢測與其它相似物質無明顯交叉反應。
        由于受到技術及樣本來源的限制,不可能完成對所有相關或相似物質交叉反應檢測,因此本試劑盒有可能與未經檢測的其它物質有交叉反應。

        回收率

        分別于定值血清及血漿樣本中加入一定量的表皮生長因子(EGF)(加標樣品),重復測定并計算其均值,回收率為測定值與理論值的比率。

        樣本 回收率范圍(%) 平均回收率(%)
        serum(n=5) 87-105 95
        EDTA plasma(n=5) 90-104 94
        heparin plasma(n=5) 92-101 98

        精密度

        精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
        批內差:取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續測定20 次,分別計算不同濃度樣本的平均值及SD值。
        批間差:選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定,每個樣本使用同一試劑盒重復測定8次,分別計算不同濃度樣本的平均值及SD值。
        批內差: CV<10%
        批間差: CV<12%

        線性

        在定值血清及血漿樣本內加入適量的表皮生長因子(EGF),并倍比稀釋成1:2,1:4,1:8,1:16的待測樣本,線性范圍即為稀釋后樣本中表皮生長因子(EGF)含量的測定值與理論值的比率。

        樣本 1:2 1:4 1:8 1:16
        serum(n=5) 96-104% 94-101% 89-96% 91-101%
        EDTA plasma(n=5) 78-96% 79-93% 91-98% 87-96%
        heparin plasma(n=5) 99-105% 93-101% 80-104% 78-101%

        穩定性

        經測定,試劑盒在有效期內按推薦溫度保存,其活性降低率小于5%。
        為減小外部因素對試劑盒破壞前后檢測值的影響,實驗室的環境條件需盡量保持一致,尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

        實驗流程

        1. 實驗前標準品、試劑及樣本的準備;
        2. 加樣(標準品及樣本)100µL,37°C孵育1小時;
        3. 吸棄,加檢測溶液A100µL,37°C孵育1小時;
        4. 洗板3次;
        5. 加檢測溶液B100µL,37°C孵育30分鐘;
        6. 洗板5次;
        7. 加TMB底物90µL,37°C孵育10-20分鐘;
        8. 加終止液50µL,立即450nm讀數。

        實驗原理

        將表皮生長因子(EGF)抗體包被于96孔微孔板中,制成固相載體,向微孔中分別加入標準品或標本,其中的表皮生長因子(EGF)與連接于固相載體上的抗體結合,然后加入生物素化的表皮生長因子(EGF)抗體,將未結合的生物素化抗體洗凈后,加入HRP標記的親和素,再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉化成藍色,并在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中的表皮生長因子(EGF)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值),計算樣品濃度。

        相關產品

        編號 適用物種:Rattus norvegicus (Rat,大鼠) 應用(僅供研究使用,不用于臨床診斷!)
        APA560Ra01 表皮生長因子(EGF)活性蛋白 Cell?culture;?Activity?Assays.
        RPA560Ra01 表皮生長因子(EGF)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
        PAA560Ra01 表皮生長因子(EGF)多克隆抗體 WB; IHC; ICC; IP.
        MAA560Ra22 表皮生長因子(EGF)單克隆抗體 WB; IHC; ICC; IP.
        MAA560Ra23 表皮生長因子(EGF)單克隆抗體 WB; IHC; ICC; IP.
        MAA560Ra25 表皮生長因子(EGF)單克隆抗體 WB; IHC; ICC; IP.
        MAA560Ra27 表皮生長因子(EGF)單克隆抗體 WB; IHC; ICC; IP.
        MAA560Ra24 表皮生長因子(EGF)單克隆抗體 WB; IHC; ICC; IP.
        MAA560Ra21 表皮生長因子(EGF)單克隆抗體 WB; IHC; ICC; IP.
        LAA560Ra72 表皮生長因子(EGF)單克隆抗體(生物素標記) WB; IHC; ICC.
        SEA560Ra 表皮生長因子(EGF)檢測試劑盒(酶聯免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
        SCA560Ra 表皮生長因子(EGF)檢測試劑盒(化學發光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
        LMA560Ra 表皮生長因子(EGF)單因子檢測試劑盒(流式熒光發光法) Magnetic Luminex Assay for Antigen Detection.
        PSA560Ra01 表皮生長因子(EGF)抗體對 ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
        KSA560Ra01 表皮生長因子(EGF)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) Main materials for "Do It (ELISA Kit) Yourself".

        參考文獻

        雜志 參考文獻
        Journal of Pediatric Surgery The influence of nutrients, biliary-pancreatic secretions, and systemic trophic hormones on intestinal adaptation in a Roux-en-Y bypass model [PubMed: 20438940]
        Biomark Insights Decreased Epidermal Growth Factor (EGF) Associated with HMGB1 and Increased Hyperactivity in Children with Autism [PubMed: PMC3623607]
        Plos one The Probiotic Mixture VSL#3 Accelerates Gastric Ulcer Healing by Stimulating Vascular Endothelial Growth Factor [PubMed: PMC3590171]
        Diabetes. Allogeneic transplantation of an adipose-derived stem cell (ASC) sheet combined with artificial skin accelerates wound healing in a rat wound model of type 2 diabetes and obesity [Pubmed:25795216]
        Eur Rev Med Pharmacol Sci.? Association of colon adenomas and skin tags: coincidence or coexistence? [Pubmed:24763889]
        Int J Clin Exp Med. The effects of self-assembling peptide RADA16 hydrogel on malignant phenotype of human hepatocellular carcinoma cell [PubMed: 26628972]
        ACS Applied Materials & Interfaces Polydopamine nanoparticles modulating stimuli-responsive PNIPAM hydrogels with cell/tissue adhesiveness. [pubmed:27709887]
        Canadian Journal of Physiology and Pharmacology The effect of midkine on growth factors and oxidative status in an experimental wound model in diabetic and healthy rats [pubmed:28177680]
        Journal of Controlled Release A DPP-IV-resistant glucagon-like peptide-2 dimer with enhanced activity against radiation-induced intestinal injury [pubmed:28522195]
        Scientific Reports Optimization of Storage Temperature for Retention of Undifferentiated Cell Character of Cultured Human Epidermal Cell Sheets [pubmed:28811665]
        International Journal of?Nanomedicine Micro-/nano-topography of selective laser melting titanium enhances adhesion and proliferation and regulates adhesion-related gene expressions of human?… [Pubmed: 30233172]
        Neoplasia Patient-derived xenograft (PDX) models of colorectal carcinoma (CRC) as a platform for chemosensitivity and biomarker analysis in personalized medicine [Pubmed: 33212364]
        Aesthet Surg J Adipose-Derived Stem Cells Improve the Aging Skin of Nude Mice by Promoting Angiogenesis and Reducing Local Tissue Water [33428732]
        Cell Death & Disease Sodium/glucose cotransporter 1-dependent metabolic alterations induce tamoxifen resistance in breast cancer by promoting macrophage M2 polarization [34006822]
        Stem Cells International The Paracrine Effect of Adipose-Derived Stem Cells Orchestrates Competition between Different Damaged Dermal Fibroblasts to Repair UVB-Induced Skin??- [33381190]
        Molecular Biology Reports Umbilical cord-derived mesenchymal stem cell conditioned medium reverses neuronal oxidative injury by inhibition of TRPM2 activation and the JNK signaling?… [Pubmed:35585377]
        Biomaterials Advances Human adipose-derived stem cell-loaded small intestinal submucosa as a bioactive wound dressing for the treatment of diabetic wounds in rats []
        Authorea Syringaresinol attenuates kidney injury by inhibition of pyroptosis via activating the Nrf2 antioxidant pathway in diabetic nephropathy []
        Biointerface Research in Applied Chemistry Beetroot Improves the Therapeutic Efficacy of Bone Marrow-Derived Mesenchymal Stem Cells in Preventing Cisplatin Induced Nephrotoxicity in Male Rats []
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        亚洲精品国产情侣AV在线,日韩精品一区二区三区色欲AV,免费看高清黄A级毛片