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      1. 催乳素(PRL)單因子檢測試劑盒(流式熒光發光法)

        Magnetic Luminex Assay Kit for Prolactin (PRL)

        LTH; Luteotropic Hormone

        • 催乳素(PRL)單因子檢測試劑盒(流式熒光發光法) 產品包裝(模擬)
        • 催乳素(PRL)單因子檢測試劑盒(流式熒光發光法) 產品包裝(模擬)
        • Certificate 通過ISO 9001、ISO 13485質量體系認證

        特異性

        本試劑盒用于檢測催乳素(PRL),經檢測與其它相似物質無明顯交叉反應。
        由于受到技術及樣本來源的限制,不可能完成對所有相關或相似物質交叉反應檢測,因此本試劑盒有可能與未經檢測的其它物質有交叉反應。

        回收率

        分別于定值血清及血漿樣本中加入一定量的催乳素(PRL)(加標樣品),重復測定并計算其均值,回收率為測定值與理論值的比率。

        樣本 回收率范圍(%) 平均回收率(%)
        serum(n=5) 89-103 99
        EDTA plasma(n=5) 90-99 93
        heparin plasma(n=5) 78-99 89
        sodium citrate plasma(n=5) 87-101 90

        精密度

        精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
        批內差:取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續測定20 次,分別計算不同濃度樣本的平均值及SD值。
        批間差:選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定,每個樣本使用同一試劑盒重復測定8次,分別計算不同濃度樣本的平均值及SD值。
        批內差: CV<10%
        批間差: CV<12%

        線性

        在定值血清及血漿樣本內加入適量的催乳素(PRL),并倍比稀釋成1:2,1:4,1:8,1:16的待測樣本,線性范圍即為稀釋后樣本中催乳素(PRL)含量的測定值與理論值的比率。

        樣本 1:2 1:4 1:8 1:16
        serum(n=5) 88-104% 79-94% 89-103% 80-103%
        EDTA plasma(n=5) 83-91% 80-102% 82-104% 97-105%
        heparin plasma(n=5) 99-105% 93-102% 89-97% 89-99%
        sodium citrate plasma(n=5) 89-104% 83-102% 86-101% 80-95%

        穩定性

        經測定,試劑盒在有效期內按推薦溫度保存,其活性降低率小于5%。
        為減小外部因素對試劑盒破壞前后檢測值的影響,實驗室的環境條件需盡量保持一致,尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

        實驗流程

        1. 實驗前標準品、試劑及樣本準備;
        2. 加樣(標準品、樣本、磁珠)標準品或樣本100μL及磁珠10μL,
            37°C酶標板振蕩器孵育90分鐘;
        3. 磁吸甩干,加檢測溶液A100μL,37°C酶標板振蕩器孵育60分鐘;
        4. 磁吸洗板3次;
        5. 加檢測溶液B100μL,37°C振動孵育30分鐘;
        6. 磁吸洗板3次;
        7. 加鞘液100μL,旋渦震蕩2分鐘后讀數。

        實驗原理

        將催乳素(PRL)抗體包被于磁珠,制成固相載體,向微孔中分別加入標準品或標本以及磁珠,其中的催乳素(PRL)與連接于固相載體上的抗體結合,然后加入生物素化的催乳素(PRL)抗體,將未結合的生物素化抗體洗凈后,加入PE標記的親和素,再次徹底洗滌后即可上機讀數。MFI值和樣品中的催乳素(PRL)呈正相關。

        贈品

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        編號 適用物種:Sus scrofa; Porcine (Pig,豬) 應用(僅供研究使用,不用于臨床診斷!)
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        LMA846Po 催乳素(PRL)單因子檢測試劑盒(流式熒光發光法) Magnetic Luminex Assay for Antigen Detection.

        參考文獻

        雜志 參考文獻
        The Journal of Biological Chemistry In Vivo Evidence for Epidermal Growth Factor Receptor (EGFR)-mediated Release of Prolactin from the Pituitary Gland [Jcb: 39297]
        Journal of Clinical Neuroscience TLR9 expression is associated with prognosis in patients with glioblastoma multiforme [ScienceDirect: S0967586811003572]
        Sensors and Actuators B: Chemical Determination of prolactin hormone in serum and urine using an electrochemical immunosensor based on poly(pyrrolepropionic acid)/carbon nanotubes hybrid modified electrodes [Sciencedirect:S0925400514000720]
        Veterinary Word Effect of extended photoperiod during winter on growth and onset of puberty in Murrah buffalo heifers [Pubmed:27051212]
        Journal?of?Dairy?Science Effect of thermal stress on physiological, hormonal and haematological parameters in Tharparkar and Karan Fries calves [publication:306322503]
        Stem Cell Research & Therapy Differentiation of human umbilical cord Wharton’s jelly-derived mesenchymal stem cells into endometrial cells [pubmed:29096715]
        Stem?Cell?Research?&?Therapy The role of mesenchymal stem cells in chemotherapy-induced gonadotoxicity [Pubmed:30021657]
        Frontiers in Molecular Neuroscience Impact of Triclosan on Female Reproduction through Reducing Thyroid Hormones to Suppress Hypothalamic Kisspeptin Neurons in Mice [Pubmed:29403355]
        Toxicology and?Applied?Pharmacology The antipsychotics sulpiride induces fatty liver in rats via phosphorylation of insulin receptor substrate-1 at Serine 307-mediated adipose tissue insulin resistance [Pubmed:29551354]
        Journal of Cellular Biochemistry The prolactin‐release inhibitor paeoniflorin suppresses proliferation and induces apoptosis in prolactinoma cells via the mitochondria‐dependent pathway [Pubmed:29388711]
        Journal?of?Cellular?and?Molecular?Medicine Metformin inhibits growth and prolactin secretion of pituitary prolactinoma cells and xenografts [Pubmed: 30334324]
        BMC?Veterinary Research Hormonal and metabolic indicators before and after farrowing in sows affected with postpartum dysgalactia syndrome [Pubmed: 30404636]
        International Journal of Basic and Clinical Endocrinology Expression of prolactin receptors in the duodenum, kidneys and skeletal system during physiological and sulpiride-induced hyperprolactinaemia [Pubmed: 30143940]
        MORPHOLOGY AND PATHOMORPHOLOGY Morphological and Biochemical Characteristics of Prostate Hyperplasia during Sulpiride Treatment [Pubmed: 32152847]
        JOURNAL OF DAIRY SCIENCE Effect of heat stress during the early and late dry period on mammary gland development of Holstein dairy cattle [Pubmed: 32684470]
        BioMed Research International Pathophysiological Changes in Female Rats with Estrous Cycle Disorder Induced by Long-Term Heat Stress [Pubmed: 32685488]
        Vet Microbiol Prolactin affects the disappearance of ALV-J viremia in vivo and inhibits viral infection [34391195]
        Antioxidants (Basel) Effects of a Nanoencapsulated Moringa Leaf Ethanolic Extract on the Physiology, Metabolism and Reproductive Performance of Rabbit Does during Summer [34439574]
        Nutrients Yogurt Enriched with Inulin Ameliorated Reproductive Functions and Regulated Gut Microbiota in Dehydroepiandrosterone-Induced Polycystic Ovary Syndrome Mice [Pubmed:35057459]
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